利用稳定同位素探针鉴定地下水中好氧降解 ETBE 的微生物

IF 1.8 4区 环境科学与生态学 Q3 WATER RESOURCES
Henry C.G. Nicholls, H. Emma Mallinson, Steven F. Thornton, Markus Hjort, Stephen A. Rolfe
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引用次数: 0

摘要

目前已发现的具有降解环境中的乙基叔丁基醚 (ETBE)能力的微生物数量有限。了解降解 ETBE 的微生物的特征和分布情况,对于在 ETBE 释放地实施自然衰减和生物修复等管理措施非常重要。本研究利用 DNA 稳定同位素探针(SIP)技术,在使用 ETBE 释放点的地下水和含水层材料构建的实验室微生态系统中,鉴定了能够有氧降解 13C 标记 ETBE 的微生物。经鉴定,γ-proteobacteria 类、β-proteobacteriales 目、Burkholderiaceae 科、Methylibium 和 Leptothrix 两种微生物是 ETBE 的主要降解者。通过对同一地点的微生 物群进行高通量测序,确定了 ETBE 响应微生物(添加 ETBE 后丰度增加的微生物),将其与 ETBE 响应微生物(添加 ETBE 后丰度增加的微生物)进行比较,发现只有一小部分 ETBE 响应微生物是 SIP 法确定的一级降解生物。从分类学角度看,ETBE 降解菌与能够降解其他汽油成分(但不能降解 ETBE)的微生物有亲缘关系,这意味着这些微生物获得了 eth 基因簇,从而具备了降解 ETBE 的功能。在 ETBE 释放地点也能发现这些 ETBE 降解菌,但相对数量较少,而且一般只在建立了微生态系统的地点发现。因此,我们建议对 ETBE 污染地进行分子研究时,应将重点放在功能基因(即 ETBE 基因簇)上,而不是特定的类群上。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Identification of Aerobic ETBE-Degrading Microorganisms in Groundwater Using Stable Isotope Probing

Identification of Aerobic ETBE-Degrading Microorganisms in Groundwater Using Stable Isotope Probing

A limited number of microorganisms have been identified with the capability to degrade ethyl tert-butyl ether (ETBE) in the environment. Knowledge of the identity and distribution of ETBE-degrading microorganisms is important for the implementation of management measures such as natural attenuation and bioremediation at ETBE-release sites. In this study, DNA-stable isotope probing (SIP) was used to identify microorganisms able to aerobically degrade 13C-labeled ETBE in laboratory microcosms constructed with groundwater and aquifer material from an ETBE-release site. Microorganisms in the Class γ-proteobacteria, Order β-proteobacteriales, Family Burkholderiaceae, and classified as Methylibium and Leptothrix, respectively, were identified as primary ETBE degraders. Comparisons with ETBE-responsive microorganisms (those which increased in abundance after the addition of ETBE), identified by high-throughput sequencing of microcosms established from the same site, showed that only a small proportion of the ETBE-responsive organisms were primary degraders as determined by SIP. ETBE degraders were taxonomically related to microorganisms able to degrade other gasoline components, but not ETBE, implying that this functionality results from acquisition of the eth gene cluster by these organisms. These ETBE degraders could also be identified at ETBE-release sites, but at low relative abundance and generally only in those locations from which the microcosms had been established. Therefore, we recommend that molecular investigations of ETBE-contaminated sites focus on functional genes (i.e., the eth gene cluster) rather than specific taxa.

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来源期刊
CiteScore
3.30
自引率
10.50%
发文量
60
审稿时长
>36 weeks
期刊介绍: Since its inception in 1981, Groundwater Monitoring & Remediation® has been a resource for researchers and practitioners in the field. It is a quarterly journal that offers the best in application oriented, peer-reviewed papers together with insightful articles from the practitioner''s perspective. Each issue features papers containing cutting-edge information on treatment technology, columns by industry experts, news briefs, and equipment news. GWMR plays a unique role in advancing the practice of the groundwater monitoring and remediation field by providing forward-thinking research with practical solutions.
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