Jéssica R. da Silva, Kelly C. N. R. Pedro, Gabrielle A. R. da Silva, Aline M. Castro, Maria Alice Z. Coelho, Bernardo D. Ribeiro, Cristiane A. Henriques, Marta A. P. Langone
{"title":"探索从脂肪分解亚罗菌中提取的基于脂肪酶的脂肪酸乙酯合成生物催化剂","authors":"Jéssica R. da Silva, Kelly C. N. R. Pedro, Gabrielle A. R. da Silva, Aline M. Castro, Maria Alice Z. Coelho, Bernardo D. Ribeiro, Cristiane A. Henriques, Marta A. P. Langone","doi":"10.1007/s43153-024-00492-6","DOIUrl":null,"url":null,"abstract":"<p>This work aimed to evaluate the lipases from <i>Yarrowia lipolytica</i> as a biocatalyst for synthesizing fatty acid ethyl esters (FAEE). The <i>Yarrowia lipolytica</i> lipases were evaluated in three distinct forms: firstly, as the crude enzymatic liquid extract derived from the submerged fermentation process of <i>Y. lipolytica</i> (YLL); secondly, as YLL immobilized on silica-alumina (YLL-S40); and thirdly, as the fermented solid material obtained from solid-state fermentation of <i>Y. lipolytica</i> (SEP-YLL). The highest FAEE content (54 wt.%) was obtained after 240 h in transesterification reactions using SEP-YLL. The influence of temperature, biocatalyst concentration, and reaction time was studied in the ethyl oleate synthesis. The SEP-YLL also showed better results compared to YLL and YLL-S40. The reaction catalyzed by SEP-YLL achieved 81% conversion at 30 °C. The SEP-YLL was evaluated in a batch-stirred reactor (BSTR) and a continuous packed-bed reactor (PBR), obtaining an oleic acid conversion of 80% and 70%, respectively. The low-cost biocatalyst (SEP-YLL) demonstrated potential for application in biodiesel production by reducing free fatty acids from an acid oil source through PBR and BSTR.</p>","PeriodicalId":1,"journal":{"name":"Accounts of Chemical Research","volume":null,"pages":null},"PeriodicalIF":16.4000,"publicationDate":"2024-07-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Exploring lipase-based biocatalysts derived from Yarrowia lipolytica for fatty acid ethyl esters synthesis\",\"authors\":\"Jéssica R. da Silva, Kelly C. N. R. Pedro, Gabrielle A. R. da Silva, Aline M. Castro, Maria Alice Z. Coelho, Bernardo D. Ribeiro, Cristiane A. Henriques, Marta A. P. Langone\",\"doi\":\"10.1007/s43153-024-00492-6\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p>This work aimed to evaluate the lipases from <i>Yarrowia lipolytica</i> as a biocatalyst for synthesizing fatty acid ethyl esters (FAEE). The <i>Yarrowia lipolytica</i> lipases were evaluated in three distinct forms: firstly, as the crude enzymatic liquid extract derived from the submerged fermentation process of <i>Y. lipolytica</i> (YLL); secondly, as YLL immobilized on silica-alumina (YLL-S40); and thirdly, as the fermented solid material obtained from solid-state fermentation of <i>Y. lipolytica</i> (SEP-YLL). The highest FAEE content (54 wt.%) was obtained after 240 h in transesterification reactions using SEP-YLL. The influence of temperature, biocatalyst concentration, and reaction time was studied in the ethyl oleate synthesis. The SEP-YLL also showed better results compared to YLL and YLL-S40. The reaction catalyzed by SEP-YLL achieved 81% conversion at 30 °C. The SEP-YLL was evaluated in a batch-stirred reactor (BSTR) and a continuous packed-bed reactor (PBR), obtaining an oleic acid conversion of 80% and 70%, respectively. The low-cost biocatalyst (SEP-YLL) demonstrated potential for application in biodiesel production by reducing free fatty acids from an acid oil source through PBR and BSTR.</p>\",\"PeriodicalId\":1,\"journal\":{\"name\":\"Accounts of Chemical Research\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":16.4000,\"publicationDate\":\"2024-07-19\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Accounts of Chemical Research\",\"FirstCategoryId\":\"5\",\"ListUrlMain\":\"https://doi.org/10.1007/s43153-024-00492-6\",\"RegionNum\":1,\"RegionCategory\":\"化学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"CHEMISTRY, MULTIDISCIPLINARY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Accounts of Chemical Research","FirstCategoryId":"5","ListUrlMain":"https://doi.org/10.1007/s43153-024-00492-6","RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"CHEMISTRY, MULTIDISCIPLINARY","Score":null,"Total":0}
Exploring lipase-based biocatalysts derived from Yarrowia lipolytica for fatty acid ethyl esters synthesis
This work aimed to evaluate the lipases from Yarrowia lipolytica as a biocatalyst for synthesizing fatty acid ethyl esters (FAEE). The Yarrowia lipolytica lipases were evaluated in three distinct forms: firstly, as the crude enzymatic liquid extract derived from the submerged fermentation process of Y. lipolytica (YLL); secondly, as YLL immobilized on silica-alumina (YLL-S40); and thirdly, as the fermented solid material obtained from solid-state fermentation of Y. lipolytica (SEP-YLL). The highest FAEE content (54 wt.%) was obtained after 240 h in transesterification reactions using SEP-YLL. The influence of temperature, biocatalyst concentration, and reaction time was studied in the ethyl oleate synthesis. The SEP-YLL also showed better results compared to YLL and YLL-S40. The reaction catalyzed by SEP-YLL achieved 81% conversion at 30 °C. The SEP-YLL was evaluated in a batch-stirred reactor (BSTR) and a continuous packed-bed reactor (PBR), obtaining an oleic acid conversion of 80% and 70%, respectively. The low-cost biocatalyst (SEP-YLL) demonstrated potential for application in biodiesel production by reducing free fatty acids from an acid oil source through PBR and BSTR.
期刊介绍：
Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance.
Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.
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