Argonaute CSR-1 限制了全染色体蛋白 CENP-A/HCP-3 的定位。

IF 3.3 3区 生物学 Q3 CELL BIOLOGY
Journal of cell science Pub Date : 2024-09-15 Epub Date: 2024-09-18 DOI:10.1242/jcs.261895
Charmaine Yan Yu Wong, Hok Ning Tsui, Yue Wang, Karen Wing Yee Yuen
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引用次数: 0

摘要

中心粒功能失常引起的染色体分离错误会导致染色体不稳定和非整倍体。在秀丽隐杆线虫(Caenorhabditis elegans)中,Argonaute 蛋白 CSR-1 对染色体的正常分离至关重要,但其具体机制尚未完全清楚。在这里,我们研究了 CSR-1 如何调控优雅子胚胎中的中心粒和动点心轴功能。我们发现,CSR-1 的缺失会导致有丝分裂进程和染色体相对于纺锤极的定位缺陷。CSR-1的敲除不会影响中心粒组蛋白H3变体CENP-A/HCP-3的mRNA和蛋白质水平,但会增加HCP-3和一些动点核蛋白在有丝分裂染色体上的定位。染色质 HCP-3 定位的提高取决于 CSR-1 RNAi 途径上游因子 EGO-1 和 CSR-1 的 PIWI 结构域活性。我们的研究结果表明,CSR-1 限制了全中心体的 HCP-3 水平,防止了错误的动点核组装,从而促进了染色体的准确分离。我们的研究揭示了 CSR-1 在胚胎中调控 HCP-3 在染色质上的沉积和中心粒功能的作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Argonaute protein CSR-1 restricts localization of holocentromere protein HCP-3, the C. elegans CENP-A homolog.

Chromosome segregation errors caused by centromere malfunction can lead to chromosome instability and aneuploidy. In Caenorhabditis elegans, the Argonaute protein CSR-1 is essential for proper chromosome segregation, although the specific mechanisms are not fully understood. Here, we investigated how CSR-1 regulates centromere and kinetochore function in C. elegans embryos. We found that depletion of CSR-1 results in defects in mitotic progression and chromosome positioning relative to the spindle pole. Knockdown of CSR-1 does not affect mRNA and protein levels of the centromeric histone H3 variant and CENP-A homolog HCP-3 but does increase the localization of HCP-3 and some kinetochore proteins to the mitotic chromosomes. Such elevation of HCP-3 chromatin localization depends on EGO-1, which is an upstream factor in the CSR-1 RNA interference (RNAi) pathway, and PIWI domain activity of CSR-1. Our results suggest that CSR-1 restricts the level of HCP-3 at the holocentromeres, prevents erroneous kinetochore assembly and thereby promotes accurate chromosome segregation. Our work sheds light on the role of CSR-1 in regulating deposition of HCP-3 on chromatin and centromere function in embryos.

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来源期刊
Journal of cell science
Journal of cell science 生物-细胞生物学
CiteScore
7.30
自引率
2.50%
发文量
393
审稿时长
1.4 months
期刊介绍: Journal of Cell Science publishes cutting-edge science, encompassing all aspects of cell biology.
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