胰岛素对修复糖尿病肾病大鼠肾损伤的影响

IF 3.7 2区 医学 Q2 ENDOCRINOLOGY & METABOLISM
Xinfang Xiao, Liu Wu, Juan Deng, Junfen Li, Yiqing Zhou, Sicheng He, Faqi Li, Yan Wang
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引用次数: 0

摘要

导言:糖尿病患者长期高血糖会导致糖尿病肾病(DN)的发生,并增加肾衰竭的易感性。低强度脉冲超声(LIPUS)是一种无创方式,已被证明具有有效的组织修复能力。本研究的目的是展示 LIPUS 在动物和细胞水平上对肾损伤的修复潜力,同时确定最佳脉冲长度 (PL):我们建立了 DN 大鼠模型,随后对大鼠肾脏进行超声波照射(PL=0.2 毫秒、10 毫秒、20 毫秒)。随后,我们评估了肾脏的结构和功能变化。此外,我们还诱导了荚膜细胞凋亡,并评估了超声照射后荚膜细胞凋亡的发生情况:结果:照射后,DN 大鼠的肾间质扩张和基底膜增厚得到改善。尿酸表达增加,而尿微量白蛋白、尿液中的荚膜萼萼酶、血尿素氮和血清肌酐水平下降(p结论:实验证明,LIPUS 能有效恢复 DN 大鼠的肾脏结构和功能,最佳 PL 为 0.2 毫秒。LIPUS缓解DN的机制在于其改善脂质代谢紊乱的能力。这些研究结果表明,LIPUS 可为这一领域的未来研究提供新的视角。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Effects of insonification on repairing the renal injury of diabetic nephropathy rats.

Introduction: Prolonged hyperglycemia in diabetes mellitus can result in the development of diabetic nephropathy (DN) and increase the susceptibility to kidney failure. Low-intensity pulsed ultrasound (LIPUS) is a non-invasive modality that has demonstrated effective tissue repair capabilities. The objective of this study was to showcase the reparative potential of LIPUS on renal injury at both animal and cellular levels, while also determining the optimal pulse length (PL).

Research design and methods: We established a rat model of DN, and subsequently subjected the rats' kidneys to ultrasound irradiation (PL=0.2 ms, 10 ms, 20 ms). Subsequently, we assessed the structural and functional changes in the kidneys. Additionally, we induced podocyte apoptosis and evaluated its occurrence following ultrasound irradiation.

Results: Following irradiation, DN rats exhibited improved mesangial expansion and basement membrane thickening. Uric acid expression increased while urinary microalbumin, podocalyxin in urine, blood urea nitrogen, and serum creatinine levels decreased (p<0.05). These results suggest that the optimal PL was 0.2 ms. Using the optimal PL further demonstrated the reparative effect of LIPUS on DN, it was found that LIPUS could reduce podococyte apoptosis and alleviate kidney injury. Metabolomics revealed differences in metabolites including octanoic acid and seven others and western blot results showed a significant decrease in key enzymes related to lipolysis (p<0.05). Additionally, after irradiating podocytes with different PLs, we observed suppressed apoptosis (p<0.05), confirming the optimal PL as 0.2 ms.

Conclusions: LIPUS has been demonstrated to effectively restore renal structure and function in DN rats, with an optimal PL of 0.2 ms. The mechanism underlying the alleviation of DN by LIPUS is attributed to its ability to improve lipid metabolism disorder. These findings suggest that LIPUS may provide a novel perspective for future research in this field.

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来源期刊
BMJ Open Diabetes Research & Care
BMJ Open Diabetes Research & Care Medicine-Endocrinology, Diabetes and Metabolism
CiteScore
9.30
自引率
2.40%
发文量
123
审稿时长
18 weeks
期刊介绍: BMJ Open Diabetes Research & Care is an open access journal committed to publishing high-quality, basic and clinical research articles regarding type 1 and type 2 diabetes, and associated complications. Only original content will be accepted, and submissions are subject to rigorous peer review to ensure the publication of high-quality — and evidence-based — original research articles.
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