F.J. Zijlstra , A.M.W.C. van den Broek , J.E. Vincent , P.P.H.M. Diderich , A.M.C. op de Hoek-Fez , M. Claeys
{"title":"持续动态腹膜透析患者腹膜炎期间巨噬细胞形成白三烯B4、20-羟基白三烯B4及其他花生四烯酸代谢物的研究","authors":"F.J. Zijlstra , A.M.W.C. van den Broek , J.E. Vincent , P.P.H.M. Diderich , A.M.C. op de Hoek-Fez , M. Claeys","doi":"10.1016/0262-1746(87)90067-9","DOIUrl":null,"url":null,"abstract":"<div><p>Macrophages, isolated from dialysis fluid of three patients with continuous ambulatory peritoneal dialysis (CAPD) at different times during peritonitis were labelled with <sup>14</sup>C-arachidonic acid and stimulated with the calcium ionophore A23187. The main metabolites formed by 5-lipoxygenase activity were leukotriene B<sub>4</sub> (LTB<sub>4</sub>) and 5-hydroxy-6,9,11,14-eicosatetraenoic acid (5-HETE). Smaller amounts of cyclooxygenase metabolites were present and also a major compound with an elution time between 6-keto-prostaglandin F<sub>1α</sub> (6-keto-PGF<sub>1α</sub> and thromboxane B<sub>2</sub> (TxB<sub>2</sub>). This substance was isolated, analysed by GC-MS and identified as 20-hydroxy-leukotriene B<sub>4</sub> (20-OH-LTB<sub>4</sub>). This indicates that human peritoneal macrophages obtained from CAPD not only produce leukotrienes and prostaglandins, but also the ω-hydroxylase product of LTB<sub>4</sub>, which has been demonstrated to be present in polymorphonuclear leucocytes. The activity of this enzyme was not correlated with the severity of the peritonitis.</p></div>","PeriodicalId":20720,"journal":{"name":"Prostaglandins, leukotrienes, and medicine","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"1987-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0262-1746(87)90067-9","citationCount":"6","resultStr":"{\"title\":\"Formation of leukotriene B4, 20-hydroxy leukotriene B4 and other arachidonic acid metabolites by macrophages during peritonitis in patients with continuous ambulatory peritoneal dialysis\",\"authors\":\"F.J. Zijlstra , A.M.W.C. van den Broek , J.E. Vincent , P.P.H.M. Diderich , A.M.C. op de Hoek-Fez , M. Claeys\",\"doi\":\"10.1016/0262-1746(87)90067-9\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Macrophages, isolated from dialysis fluid of three patients with continuous ambulatory peritoneal dialysis (CAPD) at different times during peritonitis were labelled with <sup>14</sup>C-arachidonic acid and stimulated with the calcium ionophore A23187. The main metabolites formed by 5-lipoxygenase activity were leukotriene B<sub>4</sub> (LTB<sub>4</sub>) and 5-hydroxy-6,9,11,14-eicosatetraenoic acid (5-HETE). Smaller amounts of cyclooxygenase metabolites were present and also a major compound with an elution time between 6-keto-prostaglandin F<sub>1α</sub> (6-keto-PGF<sub>1α</sub> and thromboxane B<sub>2</sub> (TxB<sub>2</sub>). This substance was isolated, analysed by GC-MS and identified as 20-hydroxy-leukotriene B<sub>4</sub> (20-OH-LTB<sub>4</sub>). This indicates that human peritoneal macrophages obtained from CAPD not only produce leukotrienes and prostaglandins, but also the ω-hydroxylase product of LTB<sub>4</sub>, which has been demonstrated to be present in polymorphonuclear leucocytes. The activity of this enzyme was not correlated with the severity of the peritonitis.</p></div>\",\"PeriodicalId\":20720,\"journal\":{\"name\":\"Prostaglandins, leukotrienes, and medicine\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1987-05-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/0262-1746(87)90067-9\",\"citationCount\":\"6\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Prostaglandins, leukotrienes, and medicine\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/0262174687900679\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Prostaglandins, leukotrienes, and medicine","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/0262174687900679","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Formation of leukotriene B4, 20-hydroxy leukotriene B4 and other arachidonic acid metabolites by macrophages during peritonitis in patients with continuous ambulatory peritoneal dialysis
Macrophages, isolated from dialysis fluid of three patients with continuous ambulatory peritoneal dialysis (CAPD) at different times during peritonitis were labelled with 14C-arachidonic acid and stimulated with the calcium ionophore A23187. The main metabolites formed by 5-lipoxygenase activity were leukotriene B4 (LTB4) and 5-hydroxy-6,9,11,14-eicosatetraenoic acid (5-HETE). Smaller amounts of cyclooxygenase metabolites were present and also a major compound with an elution time between 6-keto-prostaglandin F1α (6-keto-PGF1α and thromboxane B2 (TxB2). This substance was isolated, analysed by GC-MS and identified as 20-hydroxy-leukotriene B4 (20-OH-LTB4). This indicates that human peritoneal macrophages obtained from CAPD not only produce leukotrienes and prostaglandins, but also the ω-hydroxylase product of LTB4, which has been demonstrated to be present in polymorphonuclear leucocytes. The activity of this enzyme was not correlated with the severity of the peritonitis.
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