埃及患者 IL-13 rs20541、FOXP3 rs3761548 基因多态性及血清 IL-13 水平与过敏性哮喘的关系。

The Egyptian journal of immunology Pub Date : 2024-07-01 DOI:10.55133/eji.310302

Ahmed R M Hassan, A. G. M. Abdallah, N. A. Ismail, Yasmin A Fahmy

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引用次数: 0

摘要

白细胞介素 13（IL-13）基因的单核苷酸多态性（SNPs）经常与过敏性哮喘的易感性增加有关。叉头盒蛋白 P3（FOXP3）是形成调节性 T 细胞（Treg）的重要分子。改变 FOXP3 功能的基因变异可能在哮喘和其他过敏性疾病的发病中发挥作用。我们旨在确定 IL-13 rs20541、FOXP3 rs3761548 基因 SNPs 和 IL-13 血清水平与过敏性哮喘患者的关联。在这项病例对照研究中，纳入了 41 名埃及过敏性哮喘患者。年龄和性别匹配。41 名正常志愿者被视为对照组。通过聚合酶链式反应/限制性片段长度多态性技术检测了所有受试者的 IL-13 rs20541 和 FOXP3 rs3761548 SNPs。血清中 IL-13 的水平通过酶联免疫吸附试验（ELISA）进行评估。研究组间 IL-13 rs20541 SNP 的 AA 基因型有显著统计学差异（p= 0.042）。此外，AA 基因型与 GG 基因型相比也有明显的统计学差异，AA 基因型患哮喘的风险是 GG 基因型的三倍（p1=0.031）（OR=3.95），A 等位基因患哮喘的风险增加了约三倍（OR=3.2）。FOXP3 rs3761548 SNP 的 AA 基因型在研究组之间存在显著统计学差异（p=0.013）。此外，AA基因型与CC基因型相比也有明显的统计学差异，AA基因型患哮喘的风险是CC基因型的7倍（p1=0.003）（OR=7.04），而A等位基因患哮喘的风险增加了约3倍（p<0.001）（OR=3.07）。IL-13的血清水平在两组之间有显著的统计学差异（P<0.001）。我们可以得出结论，IL-13 是预测过敏性哮喘的有效工具。IL-13 rs20541 基因型为 AA 和 FOXP3 rs3761548 基因型为 AA 的患者患过敏性哮喘的风险较高。

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Association of IL-13 rs20541, FOXP3 rs3761548 genes polymorphisms and serum level of IL-13 with allergic asthma in Egyptian patients.

The interleukin 13 (IL-13) gene single nucleotide polymorphisms (SNPs) are frequently linked to increased vulnerability to allergic asthma. Forkhead box protein P3 (FOXP3) is an important molecule in the formation of regulatory T cells (Treg). The genetic variants that alter FOXP3 function may have a role in the development of asthma and other allergic disorders. We aimed to determine the association of IL-13 rs20541, FOXP3 rs3761548 genes SNPs and serum levels of IL-13 with allergic asthma patients. In this case-control study, 41 Egyptian patients with allergic asthma were included. Age and gender matched. 41 normal volunteers were considered the controls. All subjects were examined for IL-13 rs20541 and FOXP3 rs3761548 SNPs by the polymerase chain reaction /restriction fragment length polymorphism technique. The serum level of IL-13 was assessed by the enzyme linked immunosorbent assay (ELISA). AA genotype at IL-13 rs20541 SNP was statistically significantly different between the studied groups (p= 0.042). Also, a statistically significant difference was detected when compared AA genotype to GG genotype as AA genotype was three times at risk for asthma (p1=0.031) (OR=3.95) and A allele increased the risk of asthma by about 3 times (OR=3.2). AA genotype at FOXP3 rs3761548 SNP was statistically significantly different between the studied groups (p=0.013). Also, a statistically significant difference was detected when compared AA genotype to CC genotype as AA genotype was 7 times at risk for asthma (p1=0.003) (OR=7.04) and A allele increased the risk of asthma by about 3 times (p<0.001) (OR=3.07). The serum level of IL-13 was statistically significant different between both groups (p<0.001). We can conclude that IL-13 could be a useful tool for predicting allergic asthma. Patients with AA genotype of IL-13 rs20541 and AA genotype of FOXP3 rs3761548 have a higher risk for developing allergic asthma.

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The Egyptian journal of immunology

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