利用β-淀粉样蛋白诱导的大鼠神经毒性评估黑木耳和木耳的体内抗老年痴呆活性

Q3 Pharmacology, Toxicology and Pharmaceutics
Prasanth Nv, P. Pandian, T. Balasubramanian
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引用次数: 0

摘要

老年痴呆症是最常见的痴呆症。细胞外的老年(淀粉样)斑块和细胞内的神经纤维缠结(NFTs)是这种疾病的特征。木犀草(Vigna radiata)和木犀草(Vigna pilosa)是许多阿育吠陀配方中用于治疗痴呆症和相关疾病的植物。本研究旨在评估这些植物对淀粉样蛋白-β(A β)1-42 模型大鼠阿尔茨海默病的神经保护作用。研究时间为 21 天。在 A β 1-42 ICV 给药后的恢复期后,从第 8 天开始,每天一次口服放射紫杉乙酸乙酯提取物和柔毛紫杉乙醇提取物(200 毫克/千克、400 毫克/千克)、多奈哌齐(3 毫克/千克),直至第 21 天。认知行为研究采用径向迷宫试验和阶梯式被动回避试验。然后对动物实施安乐死,收集脑组织并评估其抗氧化参数和脑细胞因子水平。对脑组织进行组织病理学检查。使用这些提取物治疗后,大鼠在径向臂迷宫任务和步进被动回避测试中的认知能力明显提高。过氧化脂质和一氧化氮水平的降低,以及过氧化氢酶、超氧化物歧化酶和还原型谷胱甘肽等抗氧化酶水平的升高,都表明它还能减少氧化应激。通过降低神经炎症标志物的浓度,该疗法以剂量依赖的方式缓解了大鼠的神经炎症。从研究结果中可以得出结论,通过减少氧化应激和神经炎症,木犀草和木犀属植物对改善 AD 认知障碍有益处。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Evaluation of In Vivo Anti Alzheimer's Activity of Vigna radiata and Vigna pilosa using Beta Amyloid Induced Neurotoxicity in Rats
AD is the most common form of dementia. Extracellular senile (amyloid) plaques and intracellular neurofibrillary tangles (NFTs) are hallmarks of the disease. Vigna radiata and Vigna pilosa are plants used in many Ayurvedic formulations used in the management of dementia and related conditions. The present study was aimed to evaluate the neuroprotective effect of these plants on an amyloid-β (A β ) 1-42 model of Alzheimer’s disease in rats. Duration of the study was 21 days. After the recovery period post A β 1-42 ICV administration, from the 8 th day, the ethyl acetate extract of Vigna radiata and ethanolic extract of Vigna pilosa (200mg/kg, 400mg/kg), Donepezil (3mg/kg) treatments were made once daily p.o till the 21 st day. Cognitive behavioural studies were conducted using radial maze test, Step-through Passive Avoidance Test. The animals were further subjected to euthanasia and the brain were collected and evaluated for antioxidant parameters and brain cytokine levels. The brain tissues were subjected to histopathological examination. The treatment with the extracts significantly improved the cognitive capability of the rats in the Radial arm maze task and step through passive avoidance test. It also reduced oxidative stress, which was evident by the lower levels of lipid peroxide and nitric oxide as well as elevated levels of antioxidant enzymes like catalase, superoxide dismutase and reduced glutathione. The treatment alleviated neuroinflammation in rats by decreasing the concentration of neuroinflammatory markers in a dose-dependent manner. From the results it can be concluded that the plants Vigna radiata and Vigna pilosa has beneficial effects in the improvement of cognitive impairment AD, by reducing oxidative stress and neuroinflammation.
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来源期刊
Pharmacognosy Journal
Pharmacognosy Journal Pharmacology, Toxicology and Pharmaceutics-Pharmacology
CiteScore
2.20
自引率
0.00%
发文量
151
期刊介绍: In 2004, as the PHCOG.NET – a non-profit private organization dedicated to Natural Products Research leading to develop promising drugs. Our main mission is to make information on herbal drug research readily available in different formats to suit the individual needs. Pharmacognosy Journal (Phcog J.) is one of the six journals published by Phcog.Net, Each issue covers different topics in natural product drug discovery, and also publishes manuscripts that describe pharmacognostic investigations, evaluation reports, methods, techniques and applications of all forms of medicinal plant research
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