现代埃及商用硬粒小麦(Triticum durum Desf.)和面包小麦(Triticum aestivum L.)的未成熟胚和成熟胚对体外培养的反应

Gehad Shaheen, Kasem Zaki Ahmed, Sayed Abdel-Maksoud Osman, Abdel-Raheem Tawfeek Abdel-Raheem
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引用次数: 0

摘要

小麦是世界上种植最广泛、营养价值极高的谷类作物。提高小麦的产量对于缩小埃及的粮食缺口至关重要。应用现代生物技术植物育种技术可以培育出新的高产栽培品种。组织培养是一种强大的工具,可用于促进遗传转化、诱导植物遗传变异以及培育新的改良作物品种。本研究考察了小麦栽培品种在受控实验室条件下的体外胼胝发生表达和再生能力。体外研究收集了 10 个埃及现代商业栽培品种(5 个硬粒小麦栽培品种和 5 个面包小麦栽培品种)的种子。使用两种不同的外植体(未成熟胚和成熟胚)和添加了不同植物生长调节剂的不同培养基来测试小麦胼胝体形成的最佳方案。在添加了 2 毫克/升 2,4 二氯苯氧乙酸的 MS 培养基上,未成熟胚的胼胝体形成值最高;而在添加了 3 毫克/升 2.4-D 的 MS 培养基上,成熟胚的胼胝体形成值最高。所有添加了 2,4-D 的培养基都能提高胼胝体的诱导率,这表明 2,4-D 是一种有效的生长调节剂。这项针对埃及现代栽培品种的研究结果表明,组织培养的反应受基因型、营养培养基类型以及它们之间相互作用的影响很大。胼胝体指示和植物再生最有效的外植体来源是未成熟胚。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Response of immature and mature embryos of modern Egyptian commercial durum (Triticum durum Desf.) and bread wheat (Triticum aestivum L.) for in Vitro culture
Wheat is the most extensively cultivated and extremely nutritious cereal crop in the world. Increasing wheat productivity is essential to reduce the food gap in Egypt. Applying modern biotechnological plant breeding techniques could create new, highly productive cultivars. Tissue culture is a powerful tool that can be used to facilitate genetic transformation, induce genetic variation in plants, and create new and improved crop cultivars. The present study examines in vitro callogenesis expression and regeneration capacity of wheat cultivars in controlled laboratory conditions. Seeds from ten modern Egyptian commercial cultivars (Five durum and Five bread wheat cultivars) were collected for in vitro studies. Two different explants (immature embryos and mature embryos) and different media supplemented with different plant growth regulators were used to test the best wheat callus formation protocol. Immature embryos showed the highest callus formation value on MS medium supplemented with 2 mg/l 2,4-Dichlorophenoxy acetic acid, while mature embryos showed the highest callus formation value on MS medium supplemented with 3 mg/l 2.4-D. All 2,4-D supplemented media exhibited increased callus induction, suggesting that the 2,4-D as an effective growth regulator. The results of this study with modern Egyptian cultivars demonstrated that the response to tissue culture is greatly influenced by the genotype, the type of nutrient medium, and the interaction between them. The most effective explant source for callus indication and plant regeneration is immature embryos.
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