对造成加勒比地区大规模搁浅的全深海马尾藻物种进行形态和化学分型

Phycology Pub Date : 2024-07-02 DOI:10.3390/phycology4030018
Nolwenn Kergosien, Mathieu Helias, F. Le Grand, Stéphane Cérantola, Gaëlle Simon, Charlotte Nirma, T. Thibaut, Léo Berline, T. Changeux, A. Blanfuné, S. Connan, Valérie Stiger-Pouvreau
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引用次数: 0

摘要

通过形态特征描述以及对几种代谢物的定量和定性分析,尝试具体识别在非洲和加勒比海海岸线造成大规模搁浅的热带全深海马尾藻物种的三种主要形态。在对来自北大西洋的 208 个样本进行的 25 个形态变量研究中,有 22 个变量被用于建立二分法识别钥匙,仅根据形态标准就能毫无疑问地识别出每种形态。我们还尝试使用化学指纹图谱(HR-MAS NMR)和标记来区分形态类型,具体方法包括使用高压液相色谱法分析色素含量和组成,使用薄层色谱法分析萜烯概况,使用 Folin-Ciocalteu 分析法分析酚类化合物含量,使用二维核磁共振光谱法分析结构,以及使用气相色谱法分析脂肪酸组成。虽然色素含量和组成、萜类化合物含量和酚类化合物含量无法区分,但八种脂肪酸的定量分析却能区分三种形态。此外,绿丹宁的纯化允许对其结构进行表征,从而区分三种形态。我们的研究强调了游离脂肪酸谱和叶绿单宁结构作为良好化学标记的潜力,有助于区分全深海马尾藻的三种形态。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Morpho- and Chemotyping of Holopelagic Sargassum Species Causing Massive Strandings in the Caribbean Region
The specific identification of three major morphotypes of the tropical holopelagic Sargassum species causing massive strandings on the African and Caribbean coastlines was attempted by morphological characterisation as well as quantitative and qualitative analyses of several metabolites. Of the 25 morphological variables studied on 208 samples from the North Atlantic Ocean, 22 were used to establish a dichotomous identification key, allowing without any doubt the identification of each morphotype based on their morphological criteria alone. We also attempted to differentiate morphotypes using chemical fingerprintings (HR-MAS NMR) and markers by analysing pigment level and composition using High Pressure Liquid Chromatography, terpene profiles by Thin Layer Chromatography, phenolic compound levels by the Folin-Ciocalteu assay and structures by 2D Nuclear Magnetic Resonance spectroscopy, and fatty acid composition by Gas Chromatography. While pigment level and composition, terpene profiles, and phenolic contents were not discriminating, quantification of eight fatty acids enabled the differentiation of the three morphotypes. Furthermore, phlorotannin purification permitted their structural characterisation allowing discrimination between the three morphotypes. Our study highlights the potential of the free fatty acid profile and phlorotannin structure as good chemomarkers in order to discriminate between the three morphotypes of holopelagic Sargassum.
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