Elizaveta Podshivalova, O. Sverdlova, Natalya Sharova, Daria Belova
{"title":"葡萄干饼--一种很有前景的脂肪分解酶来源","authors":"Elizaveta Podshivalova, O. Sverdlova, Natalya Sharova, Daria Belova","doi":"10.36718/1819-4036-2024-4-247-253","DOIUrl":null,"url":null,"abstract":"The purpose of the study is to evaluate the possibility of using the native microbiota of rapeseed cake as a source of new producers of lipolytic enzymes. Objectives: fermentation of rapeseed cake in the pre-sence of native microbiota; isolation of microorganism isolates; determination of their lipolytic activity. Fermentation of rapeseed cake was carried out at a hydromodulus of 1:9. The resulting suspension was kept in a shaker-incubator, then diluted 100, 1000 and 10000 times in sterile distilled water. From the resulting suspensions, consortia of microorganisms of the native microbiota of rapeseed cake were isolated; 16 isolates of microorganisms were obtained based on the morphology of the colonies. Lipolytic (twinase) activity was determined by the rapid indication method on media of various compositions. During the experiment, a solid nutrient medium was prepared with the following composition, g/l: bacterial peptone – 10; NaCl – 5; CaCl2 – 0.1; agar – 20. The medium was sterilized in an autoclave. Separately, 20 % solutions of Tween-20, -40, -60 and -80 were prepared and sterilized, after which they were mixed with an agar nutr¬ient medium under aseptic conditions and poured into sterile Petri dishes. Crops were sown on media with Tween by injection. Petri dishes with medium without inoculum were taken as a control. It was found that 10 out of 16 isolates exhibit lipolytic activity. The largest diameter of the precipitation zone was observed for group 8 and amounted to (23.2 ± 0.6) mm when incubated in a medium with TW40. The smallest dia¬meter was obtained for group 5 on a medium with TW20 (3.8 ± 0.5) mm. Further work is aimed at identi¬fying microorganisms in order to optimize the process of their cultivation and expand the range of industrial applications.","PeriodicalId":283993,"journal":{"name":"Bulletin of KSAU","volume":" 9","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2024-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"RAPESES CAKE – A PROMISING SOURCE OF LIPOLYTIC ENZYMES\",\"authors\":\"Elizaveta Podshivalova, O. Sverdlova, Natalya Sharova, Daria Belova\",\"doi\":\"10.36718/1819-4036-2024-4-247-253\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"The purpose of the study is to evaluate the possibility of using the native microbiota of rapeseed cake as a source of new producers of lipolytic enzymes. Objectives: fermentation of rapeseed cake in the pre-sence of native microbiota; isolation of microorganism isolates; determination of their lipolytic activity. Fermentation of rapeseed cake was carried out at a hydromodulus of 1:9. The resulting suspension was kept in a shaker-incubator, then diluted 100, 1000 and 10000 times in sterile distilled water. From the resulting suspensions, consortia of microorganisms of the native microbiota of rapeseed cake were isolated; 16 isolates of microorganisms were obtained based on the morphology of the colonies. Lipolytic (twinase) activity was determined by the rapid indication method on media of various compositions. During the experiment, a solid nutrient medium was prepared with the following composition, g/l: bacterial peptone – 10; NaCl – 5; CaCl2 – 0.1; agar – 20. The medium was sterilized in an autoclave. Separately, 20 % solutions of Tween-20, -40, -60 and -80 were prepared and sterilized, after which they were mixed with an agar nutr¬ient medium under aseptic conditions and poured into sterile Petri dishes. Crops were sown on media with Tween by injection. Petri dishes with medium without inoculum were taken as a control. It was found that 10 out of 16 isolates exhibit lipolytic activity. The largest diameter of the precipitation zone was observed for group 8 and amounted to (23.2 ± 0.6) mm when incubated in a medium with TW40. The smallest dia¬meter was obtained for group 5 on a medium with TW20 (3.8 ± 0.5) mm. Further work is aimed at identi¬fying microorganisms in order to optimize the process of their cultivation and expand the range of industrial applications.\",\"PeriodicalId\":283993,\"journal\":{\"name\":\"Bulletin of KSAU\",\"volume\":\" 9\",\"pages\":\"\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2024-07-04\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Bulletin of KSAU\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.36718/1819-4036-2024-4-247-253\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Bulletin of KSAU","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.36718/1819-4036-2024-4-247-253","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
RAPESES CAKE – A PROMISING SOURCE OF LIPOLYTIC ENZYMES
The purpose of the study is to evaluate the possibility of using the native microbiota of rapeseed cake as a source of new producers of lipolytic enzymes. Objectives: fermentation of rapeseed cake in the pre-sence of native microbiota; isolation of microorganism isolates; determination of their lipolytic activity. Fermentation of rapeseed cake was carried out at a hydromodulus of 1:9. The resulting suspension was kept in a shaker-incubator, then diluted 100, 1000 and 10000 times in sterile distilled water. From the resulting suspensions, consortia of microorganisms of the native microbiota of rapeseed cake were isolated; 16 isolates of microorganisms were obtained based on the morphology of the colonies. Lipolytic (twinase) activity was determined by the rapid indication method on media of various compositions. During the experiment, a solid nutrient medium was prepared with the following composition, g/l: bacterial peptone – 10; NaCl – 5; CaCl2 – 0.1; agar – 20. The medium was sterilized in an autoclave. Separately, 20 % solutions of Tween-20, -40, -60 and -80 were prepared and sterilized, after which they were mixed with an agar nutr¬ient medium under aseptic conditions and poured into sterile Petri dishes. Crops were sown on media with Tween by injection. Petri dishes with medium without inoculum were taken as a control. It was found that 10 out of 16 isolates exhibit lipolytic activity. The largest diameter of the precipitation zone was observed for group 8 and amounted to (23.2 ± 0.6) mm when incubated in a medium with TW40. The smallest dia¬meter was obtained for group 5 on a medium with TW20 (3.8 ± 0.5) mm. Further work is aimed at identi¬fying microorganisms in order to optimize the process of their cultivation and expand the range of industrial applications.