测定肉鸡饲料中白羽扇豆口味和营养特性的新方法

Vladimir Vertiprahov, Nadezhda Sergeenkova, D.D. Bezzubenko, Svetlana Polina
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摘要

本研究的目的是开发一种新方法,用于确定肉鸡蛋白质补充剂的口味和营养特性。实验对象是 20-42 天龄、患有十二指肠瘘管的 ROSS308 杂交肉鸡。在喂食 60 分钟和 120 分钟后采集十二指肠内容物,以确定蛋白质补充剂的口味(消化调节的复杂反射阶段)和营养(神经化学阶段)特性。鸡分为 4 组(每组 5 头):第 1 组对照组--接受全价饲料;第 2 组实验组--由 25.0 % 的白羽扇豆和 74.0 % 的小麦以及 1.0 % 的预混料组成的谷物混合物;第 3 组实验组--比例分别为 50.0/49.0%;第 4 组实验组--比例分别为 75.0/24.0%。结果表明,与全价饲料相比,采食由 25.0 % 白羽扇豆和 74.0 % 小麦组成的混合饲料的鸡群在采食 60 分钟后的胰蛋白酶活性提高了 37.6 %(p < 0.05)。消化率最低的是由 75.0 % 的白羽扇豆和 24.0 % 的小麦组成的谷物混合物;与全价饲料相比,食糜中的胰蛋白酶活性降低了 26.4 %(p < 0.05)。同时,在实验组(25.0 % 白羽扇豆/74.0 % 小麦)肉鸡的血清中,胰蛋白酶活性比对照组降低了 21.5 %,这表明新陈代谢过程有所减少。因此,在消化调节的不同阶段研究十二指肠内容物中胰蛋白酶活性的方法使我们能够确定有效的蛋白质补充剂。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
A NEW METHOD FOR DETERMINING THE TASTE AND NUTRITIONAL PROPERTIES OF WHITE LUPINE IN BROILER CHICKEN FEED
The purpose of the study is to develop a new method for determining the taste and nutritional properties of protein supplements for broiler chickens. The experiments were performed on 20–42-day-old broiler chickens of the ROSS308 cross with a duodenal fistula. Duodenal contents were collected 60 and 120 minutes after feeding the birds to determine the taste (complex reflex phase of digestion regulation) and nutritional (neurochemical phase) properties of the protein supplement. 4 groups of chickens were formed (5 heads each): 1st control – received complete feed; 2nd experimental – grain mixture consisting of 25.0 % white lupine and 74.0 % wheat, 1.0 % premix; 3rd experimental, respectively, the ratio is 50.0/49.0 %; 4th experimental – 75.0/24.0 %, respectively. The results showed that trypsin activity 60 minutes after feeding increased by 37.6 % (p < 0.05) in the group of chickens receiving a feed mixture consisting of 25.0 % white lupine and 74.0 % wheat, compared to complete feed. The lowest digestibility was a grain mixture consisting of 75.0 % white lupine and 24.0 % wheat; trypsin activity in chyme decreased by 26.4 % (p < 0.05) compared to complete feed. At the same time, in the blood serum of broilers of the experimental group (25.0 % white lupine/74.0 % wheat), trypsin activity decreased compared to the control group by 21.5 %, which indicates a decrease in metabolic processes. Thus, the method of studying the activity of trypsin in duodenal contents in different phases of the regulation of digestion allows us to determine an effective protein supplement.
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