Patcharaporn Kaewmong, K. Kittiwattanawong, K. Nganvongpanit, Promporn Piboon
{"title":"两只橄榄利龟侧鳞的异常模式及其与泰国安达曼海橄榄利龟(Lepidochelys olivacea Eschscholtz, 1829)种群的遗传归属关系","authors":"Patcharaporn Kaewmong, K. Kittiwattanawong, K. Nganvongpanit, Promporn Piboon","doi":"10.3390/biology13070500","DOIUrl":null,"url":null,"abstract":"Two stranded Lepidochelys-like sea turtles were rescued from the Thai Andaman Sea coastline by veterinarians of the Phuket Marine Biological Center (PMBC), one in May of 2019 and another in July of 2021. They were first identified as olive ridley turtles (Lepidochelys olivacea), as the external appearance of both turtles was closer to that species than the other four species found in the Thai Andaman Sea. In fact, when carefully examined, an unusual pattern of the lateral scutes on each turtle was observed, specifically symmetric 5/5 and asymmetric 5/6, both of which are considered rare for L. olivacea and had never been reported in the Thai Andaman Sea. In contrast, this characteristic was more common for the closely related species, Kemp’s ridley (L. kempii), although this species is not distributed in the Indo-Pacific Ocean. Thus, we further investigated their genetic information to confirm species identification using two molecular markers, namely the mtDNA control region and nDNA RAG2. The results from the mtDNA control region sequences using the Basic Local Alignment Search Tool (BLAST) indicated that both individuals exhibited a higher percent identity with L. olivacea (99.81–100.00%) rather than L. kempii (94.29–95.41%) or any other species. A phylogenetic tree confirmed that these two turtles belonged to the L. olivacea clade. Moreover, the results of RAG2 also supported the mtDNA result, as both individuals shared the same RAG2 haplotype with L. olivacea. Thus, we have concluded that the two turtles with unusual lateral scute patterns exhibited genetic consistency with their original species, L. olivacea, which has brought attention to the importance of exploring rare phenotypes in sea turtle populations residing in Thai Seas.","PeriodicalId":504576,"journal":{"name":"Biology","volume":" 28","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2024-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Unusual Patterns of Lateral Scutes in Two Olive Ridley Turtles and Their Genetic Assignment to the Thai Andaman Sea Populations of Lepidochelys olivacea Eschscholtz, 1829\",\"authors\":\"Patcharaporn Kaewmong, K. Kittiwattanawong, K. Nganvongpanit, Promporn Piboon\",\"doi\":\"10.3390/biology13070500\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Two stranded Lepidochelys-like sea turtles were rescued from the Thai Andaman Sea coastline by veterinarians of the Phuket Marine Biological Center (PMBC), one in May of 2019 and another in July of 2021. They were first identified as olive ridley turtles (Lepidochelys olivacea), as the external appearance of both turtles was closer to that species than the other four species found in the Thai Andaman Sea. In fact, when carefully examined, an unusual pattern of the lateral scutes on each turtle was observed, specifically symmetric 5/5 and asymmetric 5/6, both of which are considered rare for L. olivacea and had never been reported in the Thai Andaman Sea. In contrast, this characteristic was more common for the closely related species, Kemp’s ridley (L. kempii), although this species is not distributed in the Indo-Pacific Ocean. 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引用次数: 0
摘要
普吉海洋生物中心(PMBC)的兽医于 2019 年 5 月和 2021 年 7 月分别从泰国安达曼海海岸线救起了两只搁浅的类海龟。这两只海龟首先被确认为橄榄脊龟(Lepidochelys olivacea),因为与在泰国安达曼海发现的其他四种海龟相比,这两只海龟的外部特征更接近橄榄脊龟。事实上,经过仔细观察,我们发现每只海龟的侧鳞都有一种不寻常的形态,特别是对称的 5/5 和不对称的 5/6,这两种形态在 L. olivacea 中都被认为是罕见的,在泰国安达曼海也从未有过报道。相比之下,这一特征在近缘物种肯普氏脊龟(L. kempii)中更为常见,尽管该物种并不分布于印度洋-太平洋。因此,我们进一步研究了它们的遗传信息,利用两个分子标记(即 mtDNA 控制区和 nDNA RAG2)来确认物种鉴定。使用基本局部比对搜索工具(BLAST)对mtDNA控制区序列进行比对的结果表明,这两个个体与L. olivacea(99.81%-100.00%)而不是L. kempii(94.29%-95.41%)或其他物种具有较高的一致性。系统发生树证实这两种龟属于 L. olivacea 支系。此外,RAG2 的结果也支持 mtDNA 的结果,因为这两个个体与 L. olivacea 具有相同的 RAG2 单倍型。因此,我们得出结论,这两只具有不寻常侧鳞图案的海龟与它们的原种 L. olivacea 具有遗传一致性,这引起了人们对泰国海域海龟种群中罕见表型探索的重要性的关注。
Unusual Patterns of Lateral Scutes in Two Olive Ridley Turtles and Their Genetic Assignment to the Thai Andaman Sea Populations of Lepidochelys olivacea Eschscholtz, 1829
Two stranded Lepidochelys-like sea turtles were rescued from the Thai Andaman Sea coastline by veterinarians of the Phuket Marine Biological Center (PMBC), one in May of 2019 and another in July of 2021. They were first identified as olive ridley turtles (Lepidochelys olivacea), as the external appearance of both turtles was closer to that species than the other four species found in the Thai Andaman Sea. In fact, when carefully examined, an unusual pattern of the lateral scutes on each turtle was observed, specifically symmetric 5/5 and asymmetric 5/6, both of which are considered rare for L. olivacea and had never been reported in the Thai Andaman Sea. In contrast, this characteristic was more common for the closely related species, Kemp’s ridley (L. kempii), although this species is not distributed in the Indo-Pacific Ocean. Thus, we further investigated their genetic information to confirm species identification using two molecular markers, namely the mtDNA control region and nDNA RAG2. The results from the mtDNA control region sequences using the Basic Local Alignment Search Tool (BLAST) indicated that both individuals exhibited a higher percent identity with L. olivacea (99.81–100.00%) rather than L. kempii (94.29–95.41%) or any other species. A phylogenetic tree confirmed that these two turtles belonged to the L. olivacea clade. Moreover, the results of RAG2 also supported the mtDNA result, as both individuals shared the same RAG2 haplotype with L. olivacea. Thus, we have concluded that the two turtles with unusual lateral scute patterns exhibited genetic consistency with their original species, L. olivacea, which has brought attention to the importance of exploring rare phenotypes in sea turtle populations residing in Thai Seas.