关于活性污泥细菌在体外形成生物膜的能力的研究

A. A. Khasanova, A. Sirotkin, E. V. Perushkina
{"title":"关于活性污泥细菌在体外形成生物膜的能力的研究","authors":"A. A. Khasanova, A. Sirotkin, E. V. Perushkina","doi":"10.21285/achb.912","DOIUrl":null,"url":null,"abstract":"   The study aims to comparatively characterize in vitro biofilm formation in bacterial cultures isolated from activated sludge, as well as archival cultures capable of xenobiotics biodegradation: Alcaligenes faecalis 2, Acinetobacter guillouiae 11h, Rhodococcus erythropolis ILBIO, and Achromobacter pulmonis PNOS. An analysis of the 16S rRNA nucleotide sequence identified strains isolated from activated sludge: Paenibacillus odorifer, Bacillus subtilis, Micrococcus yunnanensis, and Bacillus proteolyticus.   The formation of biofilms by microorganisms was studied on LB medium and synthetic culture medium (with sodium acetate as a carbon source). With cell growth on LB medium, an increase in biofilm biomass was observed in Paenibacillus odorifer, Bacillus subtilis, Alcaligenes faecalis 2, and Achromobacter pulmonis PNOS. The cultivation stage duration (72 and 144 h), as well as the additional dosing of substrates, had an effect on the biofilm formation process: by 144 h of cultivation, the biomass values amounted to 0.6–1.3 optical units. An average 63–77% increase in biofilm biomass was noted for Bacillus subtilis and Paenibacillus odorifer cells as compared to the 72-hour process. At the final stage of cultivation (144 h), the values of exopolysaccharides in the matrix amounted to over 0.02 optical units for Bacillus subtilis and Paenibacillus odorifer. The metabolic activity of activated sludge bacteria forming the biofilm reached 628–3609 Fl./OD540. Thus, activated sludge microorganisms forming the biofilm were shown to retain viability and metabolic activity during growth under in vitro conditions.","PeriodicalId":20677,"journal":{"name":"Proceedings of Universities. Applied Chemistry and Biotechnology","volume":" 60","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2024-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Study on the ability of activated sludge bacteria to form biofilms in vitro\",\"authors\":\"A. A. Khasanova, A. Sirotkin, E. V. Perushkina\",\"doi\":\"10.21285/achb.912\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"   The study aims to comparatively characterize in vitro biofilm formation in bacterial cultures isolated from activated sludge, as well as archival cultures capable of xenobiotics biodegradation: Alcaligenes faecalis 2, Acinetobacter guillouiae 11h, Rhodococcus erythropolis ILBIO, and Achromobacter pulmonis PNOS. An analysis of the 16S rRNA nucleotide sequence identified strains isolated from activated sludge: Paenibacillus odorifer, Bacillus subtilis, Micrococcus yunnanensis, and Bacillus proteolyticus.   The formation of biofilms by microorganisms was studied on LB medium and synthetic culture medium (with sodium acetate as a carbon source). With cell growth on LB medium, an increase in biofilm biomass was observed in Paenibacillus odorifer, Bacillus subtilis, Alcaligenes faecalis 2, and Achromobacter pulmonis PNOS. The cultivation stage duration (72 and 144 h), as well as the additional dosing of substrates, had an effect on the biofilm formation process: by 144 h of cultivation, the biomass values amounted to 0.6–1.3 optical units. An average 63–77% increase in biofilm biomass was noted for Bacillus subtilis and Paenibacillus odorifer cells as compared to the 72-hour process. At the final stage of cultivation (144 h), the values of exopolysaccharides in the matrix amounted to over 0.02 optical units for Bacillus subtilis and Paenibacillus odorifer. The metabolic activity of activated sludge bacteria forming the biofilm reached 628–3609 Fl./OD540. Thus, activated sludge microorganisms forming the biofilm were shown to retain viability and metabolic activity during growth under in vitro conditions.\",\"PeriodicalId\":20677,\"journal\":{\"name\":\"Proceedings of Universities. Applied Chemistry and Biotechnology\",\"volume\":\" 60\",\"pages\":\"\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2024-07-07\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Proceedings of Universities. Applied Chemistry and Biotechnology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.21285/achb.912\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Proceedings of Universities. Applied Chemistry and Biotechnology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.21285/achb.912","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0

摘要

本研究旨在比较从活性污泥中分离出来的细菌培养物以及具有异种生物降解能力的档案培养物的体外生物膜形成特征:这些细菌包括:粪阿卡列氏菌 2(Alcaligenes faecalis 2)、吉鲁阿氏不动杆菌 11h(Acinetobacter guillouiae 11h)、红球菌 ILBIO(Rhodococcus erythropolis ILBIO)和肺牛杆菌 PNOS(Achromobacter pulmonis PNOS)。通过分析 16S rRNA 核苷酸序列,确定了从活性污泥中分离出来的菌株:通过分析 16S rRNA 核苷酸序列,确定了从活性污泥中分离出的菌株:Paenibacillus odorifer、枯草芽孢杆菌、云南微球菌和蛋白溶解芽孢杆菌。 研究了微生物在 LB 培养基和合成培养基(以醋酸钠为碳源)上形成生物膜的情况。随着细胞在 LB 培养基上的生长,观察到恶臭芽孢杆菌、枯草芽孢杆菌、粪钙杆菌 2 和肺牛杆菌 PNOS 的生物膜生物量有所增加。培养阶段的持续时间(72 和 144 小时)以及额外添加的基质对生物膜的形成过程有影响:培养 144 小时后,生物量值达到 0.6-1.3 光学单位。与 72 小时的过程相比,枯草芽孢杆菌和臭味杆菌细胞的生物膜生物量平均增加了 63-77%。在培养的最后阶段(144 小时),枯草芽孢杆菌和臭味杆菌基质中的外多糖含量超过了 0.02 光学单位。形成生物膜的活性污泥细菌的代谢活性达到 628-3609 Fl./OD540。因此,在体外条件下,形成生物膜的活性污泥微生物在生长过程中保持了活力和代谢活性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Study on the ability of activated sludge bacteria to form biofilms in vitro
   The study aims to comparatively characterize in vitro biofilm formation in bacterial cultures isolated from activated sludge, as well as archival cultures capable of xenobiotics biodegradation: Alcaligenes faecalis 2, Acinetobacter guillouiae 11h, Rhodococcus erythropolis ILBIO, and Achromobacter pulmonis PNOS. An analysis of the 16S rRNA nucleotide sequence identified strains isolated from activated sludge: Paenibacillus odorifer, Bacillus subtilis, Micrococcus yunnanensis, and Bacillus proteolyticus.   The formation of biofilms by microorganisms was studied on LB medium and synthetic culture medium (with sodium acetate as a carbon source). With cell growth on LB medium, an increase in biofilm biomass was observed in Paenibacillus odorifer, Bacillus subtilis, Alcaligenes faecalis 2, and Achromobacter pulmonis PNOS. The cultivation stage duration (72 and 144 h), as well as the additional dosing of substrates, had an effect on the biofilm formation process: by 144 h of cultivation, the biomass values amounted to 0.6–1.3 optical units. An average 63–77% increase in biofilm biomass was noted for Bacillus subtilis and Paenibacillus odorifer cells as compared to the 72-hour process. At the final stage of cultivation (144 h), the values of exopolysaccharides in the matrix amounted to over 0.02 optical units for Bacillus subtilis and Paenibacillus odorifer. The metabolic activity of activated sludge bacteria forming the biofilm reached 628–3609 Fl./OD540. Thus, activated sludge microorganisms forming the biofilm were shown to retain viability and metabolic activity during growth under in vitro conditions.
求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
自引率
0.00%
发文量
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信