慢性肾脏病尿液生物标志物的质谱鉴定：蛋白质组学相关初步报告

Pub Date : 2024-07-08 DOI:10.25259/ijn_255_23

S. Kademani, Prabhudas Nelaturi, Sathya Sagar Kalidas, V. B. Ballambattu, Ravikumar Sambandam

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引用次数: 0

摘要

慢性肾脏病（CKD）是一种肾功能逐渐丧失的疾病，在全球的发病率越来越高。我们的研究旨在利用尿液蛋白质组学确定潜在的进展生物标志物。尿液样本经液相色谱-质谱联用仪（LCMS）检测，并使用 STRING 分析蛋白质相互作用网络。共鉴定出 135 种蛋白质，其中 35 种根据其临床意义被列为候选蛋白质。本研究发现，EndoG、HPX、APN、AnxA1、Mic60、LONP1 和 HYOU1 等潜在标记物与肾损伤及其进展至 CKD V 期相关。

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Mass Spectrometric Identification of Urinary Biomarkers of Chronic Kidney Disease: A Proteomic-related Preliminary Report

Chronic kidney disease (CKD) is a gradual loss of kidney function and has an increased prevalence rate worldwide. Our study was intended to identify potential biomarkers of progression using urine proteomics. This preliminary study consisted of 32 patients with stage V CKD. Urine samples were subjected to liquid chromatography–mass spectrometry (LCMS), and the network of protein interaction was analyzed using STRING. A total of 135 proteins were identified, of which 35 were listed as candidates based on their clinical significance. Protein– protein interaction study provides novel insights into the functional constitution of the proteome, selecting urine as a source of biomarkers. The present study observed that the potential markers such as EndoG, HPX, APN, AnxA1, Mic60, LONP1, and HYOU1 correlate with renal damage and its progression to CKD stage V.

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