使用微卫星标记对曼尼普尔水稻品种进行遗传多样性和 DNA 指纹分析

Q4 Agricultural and Biological Sciences

ELECTRONIC JOURNAL OF PLANT BREEDING Pub Date : 2024-07-09 DOI:10.37992/2024.1502.063

Meghachandra Singh I., U. Ngangkham, K. Sarika, Y. S. Devi, Thounaojam Seileshkumar Singh, Basanta Singh T., Rishikanta Singh Kh., Lamalakshmi Devi E., Amit Kumar, Monalisa Nameirakpam, Umananda Arambam, Thokchom Diviya, Ramgopal Laha

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引用次数: 0

摘要

在基因组学和蛋白质组学技术出现之前，水稻品种的区分主要基于形态描述符（DUS）。由于 DNA 指纹技术在品种保护、分类和冲突解决方面的广泛应用，它正成为一种重要的分子标记方法。本研究使用 42 个 SSR 标记对曼尼普尔 11 个水稻品种在分子水平上的遗传多样性和变异性进行了研究。SSR 标记的基因型数据显示了四个亚群。聚类分析表明，RC Maniphou 12 和 RC Maniphou 15 非常相似。为了建立 11 个水稻品种的 DNA 指纹数据库，成功开发了三个独立的多重 PCR 系统，使用基于扩增子大小的 11 个多态 SSR 标记的不同组合。这些标记能够区分所有 11 个已发布水稻品种的不同之处。

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Genetic diversity and DNA fingerprinting of rice varieties of Manipur using microsatellite markers

Rice variety differentiation was based on morphological descriptors (DUS) before the advent of genomic and proteomic technology. DNA fingerprinting is becoming an important molecular marker approach because of its wide application in varietal protection, classification, and conflict settlement. In the present study, genetic diversity and variability among the 11 rice varieties of Manipur at the molecular level was studied using 42 SSR markers. The genotypic data of the SSR markers revealed four sub-populations. Cluster analysis indicated the close similarity between RC Maniphou 12 and RC Maniphou 15. To create a DNA fingerprinting database for 11 rice varieties, three separate multiplex PCR systems using different combinations of 11 polymorphic SSR markers based on amplicon size were successfully developed . These were able to distinguish the distinctness of all the eleven released rice varieties.

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来源期刊

ELECTRONIC JOURNAL OF PLANT BREEDING Agricultural and Biological Sciences-Soil Science

CiteScore

1.10

自引率

0.00%

发文量

153

审稿时长

25 weeks

期刊介绍： Electronic Journal of Plant Breeding (EJPB) is an official publication of Indian Socciety of Plant Breeders (ISPB). The main aim of this journal is to promote the general advancement of plant breeding and to create a forum to bring together and facilitate the exchange of information amongst plant breeders. It is published quarterly as ONLINE as OPEN ACCESS journal in the official web page www.ejplantbreeding.org. The publications are subjected into DOUBLE BLIND PEER REVIEWES. Original research articles and research communications on all areas of Plant Breeding viz., genetics, breeding, cytology, germplasm evaluation, marker assisted breeding, etc are accepted. At present Review articles are not accepted for publication.