{"title":"泰国草药提取物 Kerra™ 的抗人类免疫缺陷病毒-1 特性","authors":"Siriwan Saehlee, Supaphorn Seetaha, Wiwat Klankaew, Pussadee Srathong, K. Choowongkomon, Khuanjarat Choengpanya","doi":"10.3390/ph17070917","DOIUrl":null,"url":null,"abstract":"Kerra™, a Thai traditional herbal medicine derived from the “Tak-Ka-Si-La Scripture” and composed of nine medicinal plants, has demonstrated potential antiviral properties against HIV. This study investigated the inhibitory effects of Kerra™ on HIV-1 reverse transcriptase (RT) and its ability to prevent pseudo-HIV viral infection in HEK293 cells. The results showed that Kerra™ extract achieved a 95.73 ± 4.24% relative inhibition of HIV-1 RT, with an IC50 value of 42.66 ± 8.74 µg/mL. Docking studies revealed that key phytochemicals in Kerra™, such as oleamide, formononetin, and biochanin A, interact with several residues in the RT non-nucleoside binding pocket, contributing to their inhibitory effects. Furthermore, Kerra™ was able to reduce pseudo-HIV infection in HEK293 cells at a concentration of 10 µg/mL, suggesting its potential as a supplementary treatment for HIV.","PeriodicalId":509865,"journal":{"name":"Pharmaceuticals","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2024-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Anti-Human Immunodeficiency Virus-1 Property of Thai Herbal Extract Kerra™\",\"authors\":\"Siriwan Saehlee, Supaphorn Seetaha, Wiwat Klankaew, Pussadee Srathong, K. Choowongkomon, Khuanjarat Choengpanya\",\"doi\":\"10.3390/ph17070917\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Kerra™, a Thai traditional herbal medicine derived from the “Tak-Ka-Si-La Scripture” and composed of nine medicinal plants, has demonstrated potential antiviral properties against HIV. This study investigated the inhibitory effects of Kerra™ on HIV-1 reverse transcriptase (RT) and its ability to prevent pseudo-HIV viral infection in HEK293 cells. The results showed that Kerra™ extract achieved a 95.73 ± 4.24% relative inhibition of HIV-1 RT, with an IC50 value of 42.66 ± 8.74 µg/mL. Docking studies revealed that key phytochemicals in Kerra™, such as oleamide, formononetin, and biochanin A, interact with several residues in the RT non-nucleoside binding pocket, contributing to their inhibitory effects. Furthermore, Kerra™ was able to reduce pseudo-HIV infection in HEK293 cells at a concentration of 10 µg/mL, suggesting its potential as a supplementary treatment for HIV.\",\"PeriodicalId\":509865,\"journal\":{\"name\":\"Pharmaceuticals\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2024-07-09\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Pharmaceuticals\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.3390/ph17070917\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Pharmaceuticals","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.3390/ph17070917","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
摘要
Kerra™ 是一种泰国传统草药，源自《Tak-Ka-Si-La 经》，由九种药用植物组成，具有潜在的抗 HIV 病毒特性。本研究调查了 Kerra™ 对 HIV-1 逆转录酶（RT）的抑制作用及其在 HEK293 细胞中预防假性 HIV 病毒感染的能力。结果表明，Kerra™ 提取物对 HIV-1 RT 的相对抑制率为 95.73 ± 4.24%，IC50 值为 42.66 ± 8.74 µg/mL。对接研究显示，Kerra™中的主要植物化学物质，如油酰胺、甲萘素和生物香豆素A，与RT非核苷结合袋中的几个残基相互作用，从而产生抑制作用。此外，当 Kerra™ 的浓度为 10 µg/mL 时，它还能减少 HEK293 细胞中的假性 HIV 感染，这表明它具有作为 HIV 辅助疗法的潜力。
Anti-Human Immunodeficiency Virus-1 Property of Thai Herbal Extract Kerra™
Kerra™, a Thai traditional herbal medicine derived from the “Tak-Ka-Si-La Scripture” and composed of nine medicinal plants, has demonstrated potential antiviral properties against HIV. This study investigated the inhibitory effects of Kerra™ on HIV-1 reverse transcriptase (RT) and its ability to prevent pseudo-HIV viral infection in HEK293 cells. The results showed that Kerra™ extract achieved a 95.73 ± 4.24% relative inhibition of HIV-1 RT, with an IC50 value of 42.66 ± 8.74 µg/mL. Docking studies revealed that key phytochemicals in Kerra™, such as oleamide, formononetin, and biochanin A, interact with several residues in the RT non-nucleoside binding pocket, contributing to their inhibitory effects. Furthermore, Kerra™ was able to reduce pseudo-HIV infection in HEK293 cells at a concentration of 10 µg/mL, suggesting its potential as a supplementary treatment for HIV.
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