Tat'yana Ebel', Svetlana Mihaylova, Aleksandr Ebel'
{"title":"无香假马来草(Tripleurospermum inodorum (L.) sch.BIP.)","authors":"Tat'yana Ebel', Svetlana Mihaylova, Aleksandr Ebel'","doi":"10.36718/1819-4036-2024-5-70-76","DOIUrl":null,"url":null,"abstract":"The purpose of reseach was to study the allelopathic activity of T. inodorum in laboratory conditions against seeds and seedlings of watercress (Lepidium sativum L.). Objectives: to identify the effect of aqueous extracts of different concentrations of the dry aerial part of T. inodorum on the dynamics of germination and the initial growth of test plant seedlings; to compare the allelopathic effects of T. inodorum identified by two biotesting methods. We used classical methods for determining the allelopathic activity of plants by biotesting on seeds and seedlings of higher plants. The preparation of a concentrated (1 : 25) aqueous extract was carried out using the hot extraction method (4 g of the above-ground part of the three-rib was poured into 100 ml of boiling water and infused for 1 hour). By successively diluting the initial extract, solutions of lower concentrations were obtained (1: 50, 1: 100 and 1: 200). The results obtained indicate a high allelopathic activity of aqueous extracts of the dry aerial part of T. inodorum, which is expressed in a decrease in seed germination and inhibition of seedling growth. With an increase in the concentration of aqueous extracts of three-ribs, the sizes of the root and hypocotyl naturally decrease. Moreover, the most sensitive organ of the test plant is the root, the size of which at the weakest concentration (1: 200) decreases by almost 2 times, while the size of the hypocotyl decreases slightly. A more clear concentration dose-effect relationship was shown by the results of biotesting for root elongation. As the concentration of extracts increases, the size of watercress roots gradually decreases, and the toxicity index of aqueous extracts increases from 21.4 to 86.6 %.","PeriodicalId":283993,"journal":{"name":"Bulletin of KSAU","volume":"77 16","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2024-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"ALLELOPATHIC ACTIVITY OF THE SCENTLESS FALSE MAYWEED (TRIPLEUROSPERMUM INODORUM (L.) SCH. BIP.)\",\"authors\":\"Tat'yana Ebel', Svetlana Mihaylova, Aleksandr Ebel'\",\"doi\":\"10.36718/1819-4036-2024-5-70-76\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"The purpose of reseach was to study the allelopathic activity of T. inodorum in laboratory conditions against seeds and seedlings of watercress (Lepidium sativum L.). Objectives: to identify the effect of aqueous extracts of different concentrations of the dry aerial part of T. inodorum on the dynamics of germination and the initial growth of test plant seedlings; to compare the allelopathic effects of T. inodorum identified by two biotesting methods. We used classical methods for determining the allelopathic activity of plants by biotesting on seeds and seedlings of higher plants. The preparation of a concentrated (1 : 25) aqueous extract was carried out using the hot extraction method (4 g of the above-ground part of the three-rib was poured into 100 ml of boiling water and infused for 1 hour). By successively diluting the initial extract, solutions of lower concentrations were obtained (1: 50, 1: 100 and 1: 200). The results obtained indicate a high allelopathic activity of aqueous extracts of the dry aerial part of T. inodorum, which is expressed in a decrease in seed germination and inhibition of seedling growth. With an increase in the concentration of aqueous extracts of three-ribs, the sizes of the root and hypocotyl naturally decrease. Moreover, the most sensitive organ of the test plant is the root, the size of which at the weakest concentration (1: 200) decreases by almost 2 times, while the size of the hypocotyl decreases slightly. A more clear concentration dose-effect relationship was shown by the results of biotesting for root elongation. As the concentration of extracts increases, the size of watercress roots gradually decreases, and the toxicity index of aqueous extracts increases from 21.4 to 86.6 %.\",\"PeriodicalId\":283993,\"journal\":{\"name\":\"Bulletin of KSAU\",\"volume\":\"77 16\",\"pages\":\"\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2024-07-10\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Bulletin of KSAU\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.36718/1819-4036-2024-5-70-76\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Bulletin of KSAU","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.36718/1819-4036-2024-5-70-76","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
摘要
研究的目的是在实验室条件下研究 T. inodorum 对水芹(Lepidium sativum L.)种子和幼苗的等位疗法活性。目标:确定不同浓度的 T. inodorum 干气生植物部分水提取物对试验植物幼苗的萌芽动态和初始生长的影响;比较两种生物测试方法确定的 T. inodorum 的等位效应。我们采用传统方法,通过对高等植物的种子和幼苗进行生物测试来确定植物的等位病理活性。采用热提取法制备浓缩(1:25)水提取物(将 4 克三棱草地上部分倒入 100 毫升沸水中浸泡 1 小时)。通过连续稀释初始提取物,得到浓度较低的溶液(1:50、1:100 和 1:200)。所得结果表明,T. inodorum 干燥气生部分的水提取物具有很高的等位病理学活性,表现为降低种子发芽率和抑制幼苗生长。随着三棱水提取物浓度的增加,根部和下胚轴的尺寸自然会减小。此外,试验植物最敏感的器官是根,在最低浓度(1:200)下,根的大小几乎减小了 2 倍,而下胚轴的大小则略有减小。根系伸长的生物测试结果表明,浓度剂量效应关系更为明显。随着提取物浓度的增加,水芹根的大小逐渐减小,水提取物的毒性指数从 21.4% 增加到 86.6%。
ALLELOPATHIC ACTIVITY OF THE SCENTLESS FALSE MAYWEED (TRIPLEUROSPERMUM INODORUM (L.) SCH. BIP.)
The purpose of reseach was to study the allelopathic activity of T. inodorum in laboratory conditions against seeds and seedlings of watercress (Lepidium sativum L.). Objectives: to identify the effect of aqueous extracts of different concentrations of the dry aerial part of T. inodorum on the dynamics of germination and the initial growth of test plant seedlings; to compare the allelopathic effects of T. inodorum identified by two biotesting methods. We used classical methods for determining the allelopathic activity of plants by biotesting on seeds and seedlings of higher plants. The preparation of a concentrated (1 : 25) aqueous extract was carried out using the hot extraction method (4 g of the above-ground part of the three-rib was poured into 100 ml of boiling water and infused for 1 hour). By successively diluting the initial extract, solutions of lower concentrations were obtained (1: 50, 1: 100 and 1: 200). The results obtained indicate a high allelopathic activity of aqueous extracts of the dry aerial part of T. inodorum, which is expressed in a decrease in seed germination and inhibition of seedling growth. With an increase in the concentration of aqueous extracts of three-ribs, the sizes of the root and hypocotyl naturally decrease. Moreover, the most sensitive organ of the test plant is the root, the size of which at the weakest concentration (1: 200) decreases by almost 2 times, while the size of the hypocotyl decreases slightly. A more clear concentration dose-effect relationship was shown by the results of biotesting for root elongation. As the concentration of extracts increases, the size of watercress roots gradually decreases, and the toxicity index of aqueous extracts increases from 21.4 to 86.6 %.