评估多草药配方在控制糖尿病视网膜病变方面的作用:体内实验模型研究

IF 0.1 Q4 MEDICINE, RESEARCH & EXPERIMENTAL
Shubhangi Kale, Sachin Bhagwat, S. Tavhare, Amrut S. Salunke, S. Sathaye
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引用次数: 0

摘要

背景:长期高血糖引起的氧化应激可导致糖尿病患者出现多种并发症,如肾病、神经病变、视网膜病变、中风、缺血性心脏病等。本研究旨在发现多草药配方对糖尿病视网膜病变大鼠模型的保护作用。材料与方法将 36 只健康雄性 Wistar 大鼠随机分为四组,即正常对照组(n=6)、糖尿病组(n=10)、标准二甲双胍组(n=10)和试验药物组(n=10),试验药物组由 Triphala(Haritaki、Aamlaki、Bibhitaki)、Devdaru、用链脲佐菌素(45 毫克/千克)诱导大鼠患糖尿病后,在不进行任何治疗的情况下饲养三周以诱导糖尿病视网膜病变,然后再用试验药物(540 毫克/千克)治疗 8 周。检测 HbA1c 水平。对眼部组织匀浆进行生化分析,以评估醛糖还原酶(AR)活性、氧化应激参数(即超氧化物歧化酶(SOD)、过氧化氢酶、谷胱甘肽(GSH)、脂质过氧化物(LPO))。对视网膜组织进行了组织病理学分析。结果试验药物明显降低了 HBA1C 水平,与标准二甲双胍相当。观察到 AR 和 LPO 水平明显下降,SOD 和 GSH 水平下降不明显。组织病理学评估表明,视网膜组织细胞稳定性得到恢复和增强。结论该草药配方具有很强的降血糖和抗氧化作用。它可用作预防和治疗糖尿病视网膜病变的辅助药物。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Assessment of polyherbal formulations in managing diabetic retinopathy: An in-vivo experimental model study
Background: Chronic hyperglycemia induced oxidative stress may leads to many complications in diabetic patient i.e. nephropathy, neuropathy, retinopathy, stroke, ischemic heart diseases etc. The present study was designed to find the protective effect of polyherbal formulation in rat model of diabetic retinopathy. Material & Methods: Healthy 36 male Wistar rats were randomly divided into four groups namely normal control (n=6), diabetic (n=10), standard metformin (n=10) and test drug (n=10) consisting Triphala (Haritaki, Aamlaki, Bibhitaki), Devdaru, Daruharidra and Musta After induction of diabetes with streptozotocin (45mg/kg), rats were kept without any treatment for period of three weeks for induction of diabetic retinopathy followed by treatment with test drug (540mg/kg) for further 8weeks. HbA1c level was tested. Eye tissue homogenates were subjected to biochemical analysis to evaluate the aldose reductase (AR) activity, oxidative stress parameters namely Superoxide dismutase (SOD), catalase, glutathione (GSH), lipid peroxidation (LPO). Histopathological analysis of retinal tissue was conducted. Results: Test drug showed significant reduction in HBA1C level equivalent to standard metformin. Significant reduction was observed in AR and LPO levels and non-significant reduction in SOD, GSH were observed. Histopathological evaluation indicative of restoration and enhancement in cellular stability of retinal tissues. Conclusion: The herbal formulation showed potent hypoglycemic and antioxidant effect. It can be used as an adjuvant medication in prevention and treatment of diabetic retinopathy.
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来源期刊
International Journal of Ayurvedic Medicine
International Journal of Ayurvedic Medicine MEDICINE, RESEARCH & EXPERIMENTAL-
自引率
50.00%
发文量
87
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