Shh 基因调控真皮乳头细胞的增殖和凋亡,影响其在羊绒山羊继发性毛囊生长周期中的差异表达

Animals Pub Date : 2024-07-12 DOI:10.3390/ani14142049
Junjie Zhang, Yujing Liu, Jiale Chang, Ru Zhang, Zhaomin Liu, Jiayue Liang, Dong Wang, Juan Feng, Wei Zhao, Hongmei Xiao
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引用次数: 0

摘要

音速刺猬(Shh)是刺猬信号通路的一个组成部分,在调节细胞增殖、分化、凋亡和受损生物体的修复方面发挥着重要作用。为进一步阐明Shh基因在羊绒山羊次生毛囊生长周期中的表达模式及其对次生毛囊乳头细胞的作用机制,提高羊绒品质,本研究以内蒙古阿尔巴斯白绒山羊为研究对象,采集不同生长阶段的皮肤样本,获得次生毛囊,通过RT-qPCR、Western印迹、免疫组化等技术检测Shh及其基因的表达,并对DPCs进行体外培养。构建了Shh基因过表达和干扰载体,并通过细胞转染技术研究了Shh基因对DPCs增殖和凋亡的影响。结果表明,Shh及其基因在羊绒山羊次级毛囊生长周期皮肤中的表达存在显著差异,在生长期表达水平最高,其次是衰老期,而在休止期表达水平最低。Shh 主要在内侧根鞘、外侧根鞘和次生毛囊乳头中表达。与干扰组相比,过表达 Shh 基因后,毛乳头细胞的增殖和活力增强。Shh基因干扰后,细胞的凋亡率增加,说明Shh基因能调控下游Ptch、Smo和Gli2基因的表达,促进DPCs的增殖,从而形成其在羊绒山羊次级毛囊生长周期中的表达模式。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Shh Gene Regulates the Proliferation and Apoptosis of Dermal Papilla Cells to Affect Its Differential Expression in Secondary Hair Follicle Growth Cycle of Cashmere Goats
Sonic hedgehog (Shh) is a component of the Hedgehog signaling pathway, playing an important role in regulating cell proliferation, differentiation, apoptosis, and the repair of damaged organisms. To further clarify the expression pattern of Shh gene in the secondary hair follicle growth cycle of cashmere goats and its mechanism of action on secondary hair follicle papilla cells, and improve cashmere quality, in this study, we took Inner Mongolia Albas white cashmere goats as the research objects and collected skin samples at different growth stages to obtain secondary hair follicles, detected Shh and its gene expression by RT-qPCR, Western blot, immunohistochemistry, and other techniques, while we also cultured DPCs in vitro. Shh gene overexpression and interference vectors were constructed, and the effects of Shh gene on the proliferation and apoptosis of DPCs were studied through cell transfection technology. The results showed that there are significant differences in Shh and its gene expression in the secondary hair follicle growth cycle skins of cashmere goats, with the highest expression level in anagen, followed by catagen, and the lowest expression level in telogen. Shh was mainly expressed in the inner root sheath, outer root sheath, and secondary hair follicle papilla. After the overexpression of Shh gene, the proliferation and vitality of the hair papilla cells were enhanced compared to the interference group. After Shh gene interference, the apoptosis rate of the cells increased, indicating that Shh gene can regulate downstream Ptch, Smo, and Gli2 gene expression to promote the proliferation of DPCs, and thus form its expression pattern in the secondary hair follicle growth cycle of cashmere goats.
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