郭敏煎剂在斑马鱼模型中的促血管生成作用

Ping-Hsun Lu, S. Tung, Chi-Chung Wen, Ching-Yuan Huang, Jhih-Wei Huang, Chih-Hsin Chen, Yau-Hung Chen
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引用次数: 0

摘要

摘要 郭敏煎(GMD)是一种能调节过敏相关症状的中药。虽然有报道称郭敏汤可通过调节免疫反应来治疗过敏相关症状,但郭敏汤治疗与血管生成之间的关系尚未见报道。我们的目的是研究 GMD 的血管生成调节活性。 在本研究中,我们利用荧光记录、碱性磷酸酶(AP)活性染色和实时聚合酶链反应(PCR)实验,在斑马鱼模型中研究了GMD对血管生成的影响。 经 GMD 处理的斑马鱼胚胎在尾静脉丛中表现出更多的毛细血管间隙(模拟:11.1 ± 1.8 [n = 20;n:胚胎数];经 GMD 处理的斑马鱼胚胎在尾静脉丛中表现出更多的毛细血管间隙(模拟:16.2 ± 1.9 [n = 20;n:胚胎数]):16.2 ± 1.9 [n = 20]).AP 活性染色显示,GMD 和甘草(GMD 的成分之一)可诱导肠下静脉生长。然而,甘草(甘草的一种成分)对斑马鱼没有明显的促血管生成作用。此外,实时 PCR 实验表明,暴露于 GMD 可能会通过调节血管生成相关基因(cdh5、nrp1a 和 flt1)的表达。 基于这些观察结果,我们提出 GMD 在斑马鱼模型中具有促进血管生成的活性,而这可能部分是由其中一种成分甘草促成的。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Pro-angiogenic effects of Guo Min decoction in a zebrafish model
ABSTRACT Guo Min decoction (GMD) is a Chinese traditional medicine that can regulate allergy-related symptoms. Although GMD treatment was reported to treat allergy-associated symptoms by regulating the immune response, the rationale between GMD treatment and angiogenesis has not been reported yet. Our objective is to investigate the angiogenesis-modulating activity of GMD. In this study, we used fluorescence recording, alkaline phosphatase (AP) activity staining, and real-time polymerase chain reaction (PCR) experiments to examine the effects of GMD on angiogenesis in a zebrafish model. GMD-treated zebrafish embryos exhibited more intercapillary spaces in the caudal vein plexus (Mock: 11.1 ± 1.8 [n = 20; n: numbers of embryos]; GMD-treated: 16.2 ± 1.9 [n = 20]). AP activity staining showed that treatment with GMD and liquorice (Gan Cao, a component of GMD) induced subintestinal vein outgrowth. However, glycyrrhizin (a component of Gan Cao) had no obvious pro-angiogenic effects on zebrafish. Furthermore, real-time PCR experiments indicated that GMD exposure might be through regulating angiogenesis-related genes (cdh5, nrp1a, and flt1) expressions. Based on these observations, we proposed that GMD had pro-angiogenic activity in a zebrafish model, and it might partially be contributed by one of the components, liquorice.
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