摩洛哥两个地区出产的月桂精油（Laurus nobilis L. Essential Oils from Two Regions of Morocco）对大茧蜂（Callosobruchus maculatus）（鞘翅目：布鲁沁科）的杀虫特性和化学特性分析

Q2 Agricultural and Biological Sciences

Agriculture Pub Date : 2024-07-15 DOI:10.3390/agriculture14071150

Rachid El Baghazaoui, Saadia Belmalha, Abdellatif Boutagayout, Laila Nassiri, Salma El Alami, J. Savoie, E. Bouiamrine

{"title":"摩洛哥两个地区出产的月桂精油（Laurus nobilis L. Essential Oils from Two Regions of Morocco）对大茧蜂（Callosobruchus maculatus）（鞘翅目：布鲁沁科）的杀虫特性和化学特性分析","authors":"Rachid El Baghazaoui, Saadia Belmalha, Abdellatif Boutagayout, Laila Nassiri, Salma El Alami, J. Savoie, E. Bouiamrine","doi":"10.3390/agriculture14071150","DOIUrl":null,"url":null,"abstract":"Morocco is a significant botanical reservoir that boasts a wealth of raw materials with promising applications across various industrial sectors, notably in pharmaceuticals and food. The objective of this study was to assess the effectiveness of essential oils (EOs) derived from Laurus nobilis L. leaves originating from the Tanger (EOT) and Meknes (EOM) regions in combating Callosobruchus maculatus infection. The chemical compositions of these oils were examined using Fourier transform infrared (FTIR) spectroscopy and gas chromatography–mass spectrometry (GC-MS). The biological activity of the EOs was evaluated via repulsion and fumigation tests against C. maculatus at varying concentrations. FTIR analysis revealed distinct vibrational bands indicative of various chemical compounds. GC-MS analysis was used to delineate the major chemical constituents of the EOs. The three predominant compounds in the EOT were 1,8-cineole (37.64%), linalool (16.40%), and adamantane (12.00%), whereas 1,8-cineole (47.84%), toluene (17.60%), and α-phellandrene (8.44%) were the most abundant in the EOM. Notably, the EOs exhibited significant repellent activity against C. maculatus, with repulsion percentages ranging from 51.11 to 90.00% in Tanger and 67.78 to 93.33% in Meknes. Mortality rates varied from 0 to 100% depending on the treatment. However, the mean concentrations showed mortality rates ranging from 29.44 to 65.56% for the EOT and from 21.11 to 67.78% for the EOM, with LD50 values of 11.96 μL/L and 5.22 μL/L. Docking studies revealed that 1,8-cineole had the highest binding affinity for the active site of acetylcholinesterase, thus confirming its toxic activity against C. maculatus. The findings of this study highlight the ability of EOs extracted from L. nobilis in the Moroccan regions of Tanger and Meknes to act as effective insecticides and repellents against C. maculatus, thereby highlighting avenues for further exploration of pest management and agricultural practices.","PeriodicalId":7447,"journal":{"name":"Agriculture","volume":"57 16","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2024-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Insecticidal Properties and Chemical Characterization of Laurus nobilis L. Essential Oils from Two Regions of Morocco against Callosobruchus maculatus (Coleoptera: Bruchinae)\",\"authors\":\"Rachid El Baghazaoui, Saadia Belmalha, Abdellatif Boutagayout, Laila Nassiri, Salma El Alami, J. Savoie, E. Bouiamrine\",\"doi\":\"10.3390/agriculture14071150\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Morocco is a significant botanical reservoir that boasts a wealth of raw materials with promising applications across various industrial sectors, notably in pharmaceuticals and food. The objective of this study was to assess the effectiveness of essential oils (EOs) derived from Laurus nobilis L. leaves originating from the Tanger (EOT) and Meknes (EOM) regions in combating Callosobruchus maculatus infection. The chemical compositions of these oils were examined using Fourier transform infrared (FTIR) spectroscopy and gas chromatography–mass spectrometry (GC-MS). The biological activity of the EOs was evaluated via repulsion and fumigation tests against C. maculatus at varying concentrations. FTIR analysis revealed distinct vibrational bands indicative of various chemical compounds. GC-MS analysis was used to delineate the major chemical constituents of the EOs. The three predominant compounds in the EOT were 1,8-cineole (37.64%), linalool (16.40%), and adamantane (12.00%), whereas 1,8-cineole (47.84%), toluene (17.60%), and α-phellandrene (8.44%) were the most abundant in the EOM. Notably, the EOs exhibited significant repellent activity against C. maculatus, with repulsion percentages ranging from 51.11 to 90.00% in Tanger and 67.78 to 93.33% in Meknes. Mortality rates varied from 0 to 100% depending on the treatment. However, the mean concentrations showed mortality rates ranging from 29.44 to 65.56% for the EOT and from 21.11 to 67.78% for the EOM, with LD50 values of 11.96 μL/L and 5.22 μL/L. Docking studies revealed that 1,8-cineole had the highest binding affinity for the active site of acetylcholinesterase, thus confirming its toxic activity against C. maculatus. The findings of this study highlight the ability of EOs extracted from L. nobilis in the Moroccan regions of Tanger and Meknes to act as effective insecticides and repellents against C. maculatus, thereby highlighting avenues for further exploration of pest management and agricultural practices.\",\"PeriodicalId\":7447,\"journal\":{\"name\":\"Agriculture\",\"volume\":\"57 16\",\"pages\":\"\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2024-07-15\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Agriculture\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.3390/agriculture14071150\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"Agricultural and Biological Sciences\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Agriculture","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.3390/agriculture14071150","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"Agricultural and Biological Sciences","Score":null,"Total":0}

引用次数: 0

摘要

摩洛哥是一个重要的植物宝库，拥有丰富的原材料，有望应用于各个工业领域，特别是制药和食品领域。本研究的目的是评估从丹吉尔（EOT）和梅克内斯（EOM）地区的月桂叶中提取的精油（EOs）在抗Callosobruchus maculatus感染方面的功效。使用傅立叶变换红外光谱法（FTIR）和气相色谱-质谱法（GC-MS）对这些精油的化学成分进行了研究。通过斥力和熏蒸试验，评估了不同浓度的桉叶油对斑马鱼的生物活性。傅立叶变换红外光谱分析显示了各种化合物的明显振动带。气相色谱-质谱分析用于确定环氧乙烷的主要化学成分。EOT 中最主要的三种化合物是 1,8-松油醇（37.64%）、芳樟醇（16.40%）和金刚烷（12.00%），而 EOM 中含量最高的是 1,8-松油醇（47.84%）、甲苯（17.60%）和α-黄柏烯（8.44%）。值得注意的是，环氧乙烷对斑马鱼具有显著的驱避活性，在坦吉尔的驱避率为 51.11% 至 90.00%，在梅克内斯的驱避率为 67.78% 至 93.33%。不同处理的死亡率从 0 到 100%不等。然而，平均浓度显示，EOT 的死亡率为 29.44% 至 65.56%，EOM 的死亡率为 21.11% 至 67.78%，半数致死剂量分别为 11.96 μL/L 和 5.22 μL/L。对接研究显示，1,8-蒎烯与乙酰胆碱酯酶活性位点的结合亲和力最高，从而证实了其对斑马鱼的毒性活性。这项研究的结果突出表明，从摩洛哥丹吉尔和梅克内斯地区的 L. nobilis 中提取的环氧乙烷能够作为有效的杀虫剂和驱虫剂来对付斑潜蝇，从而为进一步探索害虫管理和农业实践指明了道路。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

查看原文本刊更多论文

Insecticidal Properties and Chemical Characterization of Laurus nobilis L. Essential Oils from Two Regions of Morocco against Callosobruchus maculatus (Coleoptera: Bruchinae)

Morocco is a significant botanical reservoir that boasts a wealth of raw materials with promising applications across various industrial sectors, notably in pharmaceuticals and food. The objective of this study was to assess the effectiveness of essential oils (EOs) derived from Laurus nobilis L. leaves originating from the Tanger (EOT) and Meknes (EOM) regions in combating Callosobruchus maculatus infection. The chemical compositions of these oils were examined using Fourier transform infrared (FTIR) spectroscopy and gas chromatography–mass spectrometry (GC-MS). The biological activity of the EOs was evaluated via repulsion and fumigation tests against C. maculatus at varying concentrations. FTIR analysis revealed distinct vibrational bands indicative of various chemical compounds. GC-MS analysis was used to delineate the major chemical constituents of the EOs. The three predominant compounds in the EOT were 1,8-cineole (37.64%), linalool (16.40%), and adamantane (12.00%), whereas 1,8-cineole (47.84%), toluene (17.60%), and α-phellandrene (8.44%) were the most abundant in the EOM. Notably, the EOs exhibited significant repellent activity against C. maculatus, with repulsion percentages ranging from 51.11 to 90.00% in Tanger and 67.78 to 93.33% in Meknes. Mortality rates varied from 0 to 100% depending on the treatment. However, the mean concentrations showed mortality rates ranging from 29.44 to 65.56% for the EOT and from 21.11 to 67.78% for the EOM, with LD50 values of 11.96 μL/L and 5.22 μL/L. Docking studies revealed that 1,8-cineole had the highest binding affinity for the active site of acetylcholinesterase, thus confirming its toxic activity against C. maculatus. The findings of this study highlight the ability of EOs extracted from L. nobilis in the Moroccan regions of Tanger and Meknes to act as effective insecticides and repellents against C. maculatus, thereby highlighting avenues for further exploration of pest management and agricultural practices.

求助全文

通过发布文献求助，成功后即可免费获取论文全文。去求助

来源期刊

Agriculture Agricultural and Biological Sciences-Horticulture

CiteScore

1.90

自引率

0.00%

发文量

审稿时长

11 weeks

期刊介绍： The Agriculture (Poľnohospodárstvo) is a peer-reviewed international journal that publishes mainly original research papers. The journal examines various aspects of research and is devoted to the publication of papers dealing with the following subjects: plant nutrition, protection, breeding, genetics and biotechnology, quality of plant products, grassland, mountain agriculture and environment, soil science and conservation, mechanization and economics of plant production and other spheres of plant science. Journal is published 4 times per year.