分子方法证实了巴西南部首次报告的非土著 Perna viridis Linnaeus, 1758 (Mytilida, Mytilidae)

Check List Pub Date : 2024-07-16 DOI:10.15560/20.4.859
Mayara Carneiro Beltrão, Natalia Joana Rebello da Cunha, Yan de Oliveira Laaf, Fernando Luiz Diehl, Tayse Demetro dos Santos
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引用次数: 0

摘要

贻贝 Perna viridis,俗称绿贻贝,原产于印度-太平洋地区,后被引入全球多个国家。在巴西,里约热内卢州和塞阿拉州已有该物种的记录。为了评估巴西南部地区贻贝的存在情况,我们在巴拉那州的巴拉那瓜河口综合区采集了 14 只贻贝。这些贻贝被发现附着在 Ponta do Poço 码头的人工建筑上,深度为 2 米。使用商业试剂盒提取 DNA,使用引物 dgL-CO-1490 和 dgHCO-2198 通过 PCR 扩增 COI 基因,使用 Sanger 方法测序,并通过 BOLD 系统鉴定物种。使用 MEGA 11 软件,利用来自 3 个 Perna 物种的 28 条序列构建了系统发生树。因此,本研究通过分子鉴定首次证实了巴西鲈鱼(P. viridis)的存在,并增加了巴西第二个有记录的州。巴西海岸线为 P. viridis 的建立和发展提供了最佳环境条件。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Molecular methods confirm the first report of the non-indigenous Perna viridis Linnaeus, 1758 (Mytilida, Mytilidae) in southern Brazil
The mussel Perna viridis, commonly known as Green Mussel, is native from the Indo-Pacific region and has been introduced in various countries around the globe. In Brazil, the species has already been recorded in Rio de Janeiro and Ceará states. With the aim of assessing the presence of mussels in the southern region of the country, 14 individuals were collected in the Paranaguá Estuarine Complex, Paraná. The mussels were found attached at a depth of 2 m on the artificial structure of Ponta do Poço Marina. The DNA was extracted using a commercial kit, the COI gene was amplified through PCR using the primers dgL-CO-1490 and dgHCO-2198, sequenced by the Sanger method, and the species was identified through the BOLD Systems. The phylogenetic tree was built on the software MEGA 11 using 28 sequences from three Perna species. Therefore, the present study confirms the occurrence of P. viridis in Brazil through molecular identification for the first time and adds a second Brazilian state where it has been recorded. The Brazilian coastline provides optimal environmental conditions for the establishment and development of P. viridis. 
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