揭示假双歧杆菌代谢某些植物源聚糖的途径

Rocio Sanchez-Gallardo, Francesca Bottacini, Lisa Friess, M. Esteban-Torres, Clarissa Somers, Rebecca L. Moore, F. McAuliffe, Paul D. Cotter, D. van Sinderen
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引用次数: 0

摘要

双歧杆菌是人类肠道微生物群中常见的成员,它们拥有代谢某些植物源复杂碳水化合物所需的酶机制。在目前的研究中,我们描述了 21 株新分离的双歧杆菌在各种植物衍生糖类上引起的不同生长特征。通过基因性状匹配和比较基因组分析,我们发现了两种不同的木聚糖酶,它们负责降解木聚糖。此外,三种不同的胞外α-淀粉酶被证明参与了假淀粉芽孢杆菌某些菌株的淀粉降解。生化鉴定表明,这三种α-淀粉酶都能分解相关的底物淀粉、直链淀粉、麦芽糊精、糖原和淀粉。编码这些酶的基因在假中华酵母菌中存在差异,因此构成了菌株对肠道环境的特异性适应,因为这些糖构成了人类饮食中常见的植物衍生碳水化合物。总之,我们的研究为了解源自人类的双歧杆菌物种对这些常见膳食碳水化合物的新陈代谢提供了见解。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Unveiling metabolic pathways of selected plant-derived glycans by Bifidobacterium pseudocatenulatum
Bifidobacteria are commonly encountered members of the human gut microbiota that possess the enzymatic machinery necessary for the metabolism of certain plant-derived, complex carbohydrates. In the current study we describe differential growth profiles elicited by a panel of 21 newly isolated Bifidobacterium pseudocatenulatum strains on various plant-derived glycans. Using a combination of gene-trait matching and comparative genome analysis, we identified two distinct xylanases responsible for the degradation of xylan. Furthermore, three distinct extracellular α-amylases were shown to be involved in starch degradation by certain strains of B. pseudocatenulatum. Biochemical characterization showed that all three α-amylases can cleave the related substrates amylose, amylopectin, maltodextrin, glycogen and starch. The genes encoding these enzymes are variably found in the species B. pseudocatenulatum, therefore constituting a strain-specific adaptation to the gut environment as these glycans constitute common plant-derived carbohydrates present in the human diet. Overall, our study provides insights into the metabolism of these common dietary carbohydrates by a human-derived bifidobacterial species.
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