从耐受性牛膝根中提取内生细菌的功能鉴定和评估,以克服地黄的单一栽培问题

Chunli Zeng, Yazhou Liu, Bianhong Zhang, Chenjing Zhang, Niu Li, Leshan Ji, Chaojie Lan, Bin Qin, Yuncheng Yang, Juanying Wang, Ting Chen, Changxun Fang, Wenxiong Lin
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摘要

从牛膝(Achyranthes bidentata)根部分离和鉴定植物生长促进内生细菌(PGPEB)对生态农业具有深远的理论和实践意义,尤其是作为生物接种剂来应对连续单一栽培带来的挑战。通过生物信息学分析,我们的研究发现,在长期单一栽培条件下,这些有益细菌在牛膝根圈土壤中的丰度明显增加。随后,我们从双戟根中分离出了 563 株内生细菌。功能表征显示,这些细菌具有多种促进植物生长的特性,包括分泌 68.01 至 73.25 mg/L 的吲哚-3-乙酸(IAA)、溶解磷和钾的能力以及对病原真菌的拮抗活性(21.54%-50.81%)。通过 16S rDNA 测序,我们确定了 9 株具有生物防治和生长促进潜力的菌株。在盆栽实验中引入合成微生物菌群(SMC),可显著增加双叶地黄的根部生物量 48.19%,以及移栽地黄的根部生物量 27.01%。这些发现为应对连作挑战提供了创新见解和策略,凸显了二叉地黄丸的 PGPEB 在生态农业中克服谷氨酰胺等非寄主植物移栽障碍的实用前景,从而促进药用植物的茁壮成长。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
The functional identification and evaluation of endophytic bacteria sourced from the roots of tolerant Achyranthes bidentata to overcome monoculture problems of Rehmannia glutinosa
The isolation and identification of plant growth-promoting endophytic bacteria (PGPEB) from Achyranthes bidentata roots have profound theoretical and practical implications in ecological agriculture, particularly as bio-inoculants to address challenges associated with continuous monoculture. Our research revealed a significant increase in the abundance of these beneficial bacteria in A. bidentata rhizosphere soil under prolonged monoculture conditions, as shown by bioinformatics analysis. Subsequently, we isolated 563 strains of endophytic bacteria from A. bidentata roots. Functional characterization highlighted diverse plant growth-promoting traits among these bacteria, including the secretion of indole-3-acetic acid (IAA) ranging from 68.01 to 73.25 mg/L, phosphorus and potassium solubilization capacities, and antagonistic activity against pathogenic fungi (21.54%−50.81%). Through 16S rDNA sequencing, we identified nine strains exhibiting biocontrol and growth-promoting potential. Introduction of a synthetic microbial consortium (SMC) in pot experiments significantly increased root biomass by 48.19% in A. bidentata and 27.01% in replanted Rehmannia glutinosa. These findings provide innovative insights and strategies for addressing continuous cropping challenges, highlighting the practical promise of PGPEB from A. bidentata in ecological agriculture to overcome replanting obstacles for non-host plants like R. glutinosa, thereby promoting robust growth in medicinal plants.
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