压力感应:生长诱导的压迫激活铜绿假单胞菌的 cAMP 信号转导

bioRxiv Pub Date : 2024-07-16 DOI:10.1101/2024.07.08.602437
Lei Ni, Yajia Huang, Yaoxin Huang, Yue Yu, Jiarui Xiong, Hui Wen, Wenwen Xiao, Haiyi Liang, Fan Jin
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引用次数: 0

摘要

细菌采用各种策略来协调种群行为,其中法定人数感应是一种行之有效的机制。在这里,我们报告了铜绿假单胞菌的一种新型种群级调控机制,我们称之为 "压力感应"。这种机制允许细菌在密闭空间中调节其集体行为,以应对生长诱导的机械压缩。我们将高灵敏度的 cAMP 生物传感器与微流控技术相结合,证明了当压缩力达到约 30 nN 时,铜绿假单胞菌细胞会通过 Pil-Chp 化学传感系统迅速提高细胞内的 cAMP 水平。这种反应会导致 III 型分泌系统(一种关键的毒力因子)的上调。法定人数感应依赖于可扩散的化学信号,与之不同的是,压力感应利用机械线索来衡量种群密度和空间限制。在细菌菌落中,这种机制会产生引人注目的 cAMP 信号空间模式,包括与菌落形态中的阶梯状结构相吻合的行进环。我们的研究结果揭示了机械压缩与细菌毒力之间以前未知的联系,为了解铜绿微囊藻如何在封闭环境中协调种群级反应提供了新的视角。这项工作还拓展了我们对机械遗传学的认识,为合成生物学和生物工程应用开辟了新的可能性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Pressorum Sensing: Growth-induced Compression Activates cAMP Signaling in Pseudomonas aeruginosa
Bacteria employ various strategies to coordinate population-level behaviors, with quorum sensing being a well-established mechanism. Here, we report a novel population-level regulatory mechanism in Pseudomonas aeruginosa, which we term ‘pressorum sensing’. This mechanism allows bacteria to modulate their collective behavior in response to growth-induced mechanical compression in confined spaces. Using a highly sensitive cAMP biosensor in combination with microfluidics, we demonstrate that when compressive forces reach approximately 30 nN, P. aeruginosa cells rapidly increases intracellular cAMP levels via the Pil-Chp chemosensory system. This response leads to up-regulation of the Type III Secretion System, a key virulence factor. Unlike quorum sensing, which relies on diffusible chemical signals, pressorum sensing utilizes mechanical cues to gauge population density and spatial confinement. In bacterial colonies, this mechanism generates striking spatial patterns of cAMP signaling, including traveling rings that coincide with step-like structures in colony morphology. Our findings reveal a previously unknown link between mechanical compression and bacterial virulence, providing new insights into how P. aeruginosa coordinates population-level responses in confined environments. This work also expands our knowledge of mechanogenetics and opens up new possibilities in synthetic biology and bioengineering applications.
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