Nikhil Raj, Anshuman Pandey, Raja Roy, Manodeep Sen, Jyotsna Agarwal
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In patients complying with case and control definition, blood, urine, and cystic fluid were collected for complete blood count, urine culture, Echinococcus IgG enzyme-linked immunosorbent assay (ELISA), and metabolomic analysis. The 17, 15, and 11 metabolites in serum, urine, and cystic fluid samples were quantified, respectively, to differentiate between case and control group.</p><p><strong>Results: </strong>In this study, we observed that there was a significant downregulation of succinate metabolite in urine samples of cases, down-regulation of five metabolites (isoleucine, valine, histidine, tyrosine and formate) and upregulation of alanine in cystic fluid of cases.</p><p><strong>Conclusion: </strong>Current study demonstrates that metabolomics can be used non-invasively for rapid diagnosis of CE. This is one of the very few studies, which used <sup>1</sup>H NMR spectroscopy, to analyze the profile of metabolites in serum, urine, and cystic fluid in cases of CE and controls.</p><p><strong>How to cite this article: </strong>Raj N, Pandey A, Roy R, <i>et al</i>. Proton Nuclear Magnetic Resonance (1H NMR) Metabolomics Study in Serum, Urine, and Cystic Fluid for Differentiating Fertility and Staging of Intra-abdominal Hydatid Cyst in Adults. Euroasian J Hepato-Gastroenterol 2024;14(1):30-34.</p>","PeriodicalId":516317,"journal":{"name":"Euroasian journal of hepato-gastroenterology","volume":"14 1","pages":"30-34"},"PeriodicalIF":0.0000,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11249894/pdf/","citationCount":"0","resultStr":"{\"title\":\"Proton Nuclear Magnetic Resonance (<sup>1</sup>H NMR) Metabolomics Study in Serum, Urine, and Cystic Fluid for Differentiating Fertility and Staging of Intra-abdominal Hydatid Cyst in Adults.\",\"authors\":\"Nikhil Raj, Anshuman Pandey, Raja Roy, Manodeep Sen, Jyotsna Agarwal\",\"doi\":\"10.5005/jp-journals-10018-1416\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Background: </strong>Cystic echinococcosis (CE) is a parasitic zoonosis caused by the tapeworm <i>Echinococcus granulosus.</i> Over the past few years, a lot of research has been done on liver illnesses using metabolomics techniques to identify biomarkers which could identify the diseases in its early stages. The present study was done to explore biomarkers in serum, urine, and cystic fluid which would help in differentiating, staging, and assessing fertility of intra-abdominal hydatid cyst by using proton nuclear magnetic resonance (<sup>1</sup>H NMR) metabolomics.</p><p><strong>Materials and methods: </strong>In the study, 28 subjects (16 cases and 12 controls) were enrolled. Staging of hydatid cysts was performed using ultrasonography. In patients complying with case and control definition, blood, urine, and cystic fluid were collected for complete blood count, urine culture, Echinococcus IgG enzyme-linked immunosorbent assay (ELISA), and metabolomic analysis. The 17, 15, and 11 metabolites in serum, urine, and cystic fluid samples were quantified, respectively, to differentiate between case and control group.</p><p><strong>Results: </strong>In this study, we observed that there was a significant downregulation of succinate metabolite in urine samples of cases, down-regulation of five metabolites (isoleucine, valine, histidine, tyrosine and formate) and upregulation of alanine in cystic fluid of cases.</p><p><strong>Conclusion: </strong>Current study demonstrates that metabolomics can be used non-invasively for rapid diagnosis of CE. This is one of the very few studies, which used <sup>1</sup>H NMR spectroscopy, to analyze the profile of metabolites in serum, urine, and cystic fluid in cases of CE and controls.</p><p><strong>How to cite this article: </strong>Raj N, Pandey A, Roy R, <i>et al</i>. Proton Nuclear Magnetic Resonance (1H NMR) Metabolomics Study in Serum, Urine, and Cystic Fluid for Differentiating Fertility and Staging of Intra-abdominal Hydatid Cyst in Adults. 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引用次数: 0
摘要
背景：囊性棘球蚴病（CE）是一种由颗粒棘球蚴绦虫引起的寄生性人畜共患病。在过去几年中，人们利用代谢组学技术对肝脏疾病进行了大量研究，以确定可在疾病早期阶段识别疾病的生物标志物。本研究旨在利用质子核磁共振（1H NMR）代谢组学探索血清、尿液和囊液中的生物标记物，这些标记物有助于腹腔内水包虫囊肿的鉴别、分期和生育能力评估：研究共纳入 28 名受试者（16 例病例和 12 例对照）。采用超声波检查对包虫囊肿进行分期。对符合病例和对照定义的患者采集血液、尿液和囊液，进行全血细胞计数、尿液培养、棘球蚴 IgG 酶联免疫吸附试验（ELISA）和代谢组学分析。对血清、尿液和囊液样本中的 17 种、15 种和 11 种代谢物分别进行了定量分析，以区分病例组和对照组：结果：在这项研究中，我们观察到病例尿液样本中的琥珀酸代谢物明显下调，病例囊液样本中的5种代谢物（异亮氨酸、缬氨酸、组氨酸、酪氨酸和甲酸盐）下调，丙氨酸上调：本研究表明，代谢组学可用于 CE 的无创快速诊断。这是极少数使用 1H NMR 光谱分析 CE 病例和对照组血清、尿液和囊液中代谢物概况的研究之一：Raj N, Pandey A, Roy R, et al. Proton Nuclear Magnetic Resonance (1H NMR) Metabolomics Study in Serum, Urine, and Cystic Fluid for Differentiating Fertility and Staging of Intra-abdominal Hydatid Cyst in Adults.Euroasian J Hepato-Gastroenterol 2024;14(1):30-34.
Proton Nuclear Magnetic Resonance (1H NMR) Metabolomics Study in Serum, Urine, and Cystic Fluid for Differentiating Fertility and Staging of Intra-abdominal Hydatid Cyst in Adults.
Background: Cystic echinococcosis (CE) is a parasitic zoonosis caused by the tapeworm Echinococcus granulosus. Over the past few years, a lot of research has been done on liver illnesses using metabolomics techniques to identify biomarkers which could identify the diseases in its early stages. The present study was done to explore biomarkers in serum, urine, and cystic fluid which would help in differentiating, staging, and assessing fertility of intra-abdominal hydatid cyst by using proton nuclear magnetic resonance (1H NMR) metabolomics.
Materials and methods: In the study, 28 subjects (16 cases and 12 controls) were enrolled. Staging of hydatid cysts was performed using ultrasonography. In patients complying with case and control definition, blood, urine, and cystic fluid were collected for complete blood count, urine culture, Echinococcus IgG enzyme-linked immunosorbent assay (ELISA), and metabolomic analysis. The 17, 15, and 11 metabolites in serum, urine, and cystic fluid samples were quantified, respectively, to differentiate between case and control group.
Results: In this study, we observed that there was a significant downregulation of succinate metabolite in urine samples of cases, down-regulation of five metabolites (isoleucine, valine, histidine, tyrosine and formate) and upregulation of alanine in cystic fluid of cases.
Conclusion: Current study demonstrates that metabolomics can be used non-invasively for rapid diagnosis of CE. This is one of the very few studies, which used 1H NMR spectroscopy, to analyze the profile of metabolites in serum, urine, and cystic fluid in cases of CE and controls.
How to cite this article: Raj N, Pandey A, Roy R, et al. Proton Nuclear Magnetic Resonance (1H NMR) Metabolomics Study in Serum, Urine, and Cystic Fluid for Differentiating Fertility and Staging of Intra-abdominal Hydatid Cyst in Adults. Euroasian J Hepato-Gastroenterol 2024;14(1):30-34.
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