咬合功能障碍中的磨蚀和牙髓形态变化。

IF 1.2 4区 医学 Q4 DEVELOPMENTAL BIOLOGY
Adi Rusu Olaru, Mihai Raul Popescu, Iancu Emil Pleşea, Mircea Sebastian Şerbănescu, Răzvan Mihail Pleşea, Melania Olimpia Cojocaru, Elena Claudia Coculescu
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引用次数: 0

摘要

目的:作者旨在评估咬合功能障碍导致的磨耗的存在和严重程度是否决定了牙髓的进一步形态变化:研究组包括45个咬合功能障碍病例的牙髓,随后分为两个亚组:24个有磨耗(AB)和21个无磨耗(NONAB)病例。牙髓形态学参数包括外层、内层和整个周边牙髓区的厚度,牙髓内是否存在钙化及其范围,牙髓内是否存在间质纤维化及其范围,以及牙髓毛细血管网的血管密度(VD)。组织样本用10%缓冲福尔马林固定,石蜡包埋,4微米切片。用3,3'-二氨基联苯胺(DAB)标记的血色素-伊红(HE)、马森三色素(MT)和抗分化簇34(CD34)抗体对连续切片进行染色,并将其转化为虚拟切片,在MATLAB(MathWorks,美国)内部专用软件的帮助下对上述参数进行比较研究。对评估参数的数值也进行了分层分类,从而得出每个参数的评分标准。所使用的统计工具有:Lilliefors 检验、t 检验(假设方差相等的双样本检验)、Mann-Whitney 检验、Pearson 相关性检验、单向方差分析(ANOVA)检验和χ²(卡方)检验:与无磨损的牙齿相比,有磨损的牙齿的外周区(PZ)及其各层都有变厚的趋势。此外,与无磨损的牙齿相比,有磨损的牙齿牙髓中有更多的钙沉积。另一方面,牙髓中的纤维组织与有无磨损没有关系。PZ 整体及其各层的演变方式相同,但无磨损牙齿组的相关性更强。钙沉积与牙髓的纤维组织数量和毛细血管密度的演变方向相反。因此,纤维组织的数量和牙髓毛细血管密度以相同的方式共同演变,在无磨损的牙齿中更为明显:我们的初步研究表明,根据咬合功能障碍牙齿的磨损程度,牙髓的不同成分会出现轻微到中等程度的变化。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Abrasion and dental pulp morphological changes in occlusal dysfunction.

Aim: The authors set out to assess if the presence and the degree of severity of the abrasion, as a consequence of the occlusal dysfunction, determine further morphological changes in the dental pulp.

Materials and methods: Study group included teeth dental pulp from 45 cases with occlusal dysfunction, subsequently divided into two subgroups: 24 cases with abrasion (AB) and 21 cases without abrasion (NONAB). The set of morphological parameters of dental pulp were thicknesses of the outer layer, inner layer and entire peripheral pulp zone, the presence of pulpal calcifications and their extent within the dental pulp, the presence of interstitial fibrosis and its extent within the dental pulp and the vascular density (VD) of pulpal capillary network. Tissue samples were fixed in 10% buffered formalin, embedded in paraffin, and sectioned off at 4 μm. Serial slides were stained with Hematoxylin-Eosin (HE), Masson's trichrome (MT) and anti-cluster of differentiation 34 (CD34) antibodies labeled with 3,3'-Diaminobenzidine (DAB) and transformed into virtual slides on which the above-mentioned parameters were studied comparatively with the help of a dedicated in-house software, realized in MATLAB (MathWorks, USA). The numerical values of the assessed parameters were also stratified in classes, thus obtaining score scales for each parameter. Statistical tools used were Lilliefors test, t-test (two-sample assuming equal variances), Mann-Whitney test, Pearson's correlation test, one-way analysis of variance (ANOVA) test and χ² (chi-squared) test.

Results and discussions: Both peripheral zone (PZ) and its layers individually tended to be thicker in the teeth with abrasion than in those without abrasion. Also, teeth with abrasion tended to develop larger amounts of calcium deposits in their dental pulp than teeth without abrasion. On the other hand, fibrotic tissue in dental pulp had no relationship with the presence or absence of abrasion. PZ as a whole and its layers evolved together in the same way, with a stronger correlation in the group of teeth without abrasion. Deposits of calcium evolved in the opposite direction to both the amount of fibrous tissue and the capillaries density of the dental pulp. Consequently, the amount of fibrous tissue and VD evolved together in the same way, more pronounced in the teeth without abrasion.

Conclusions: Our preliminary study pointed out that different components of the dental pulp showed slight to moderate changes depending on the degree of abrasion in teeth with occlusal dysfunction.

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来源期刊
CiteScore
1.70
自引率
20.00%
发文量
221
审稿时长
3-8 weeks
期刊介绍: Romanian Journal of Morphology and Embryology (Rom J Morphol Embryol) publishes studies on all aspects of normal morphology and human comparative and experimental pathology. The Journal accepts only researches that utilize modern investigation methods (studies of anatomy, pathology, cytopathology, immunohistochemistry, histochemistry, immunology, morphometry, molecular and cellular biology, electronic microscopy, etc.).
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