脐带血 CD34+ MPs 对造血细胞氧化作用的分子和生化评估

IF 2.1 4区 医学 Q3 HEMATOLOGY
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引用次数: 0

摘要

脐带血是异基因造血干细胞移植的移植物来源,其中含有脐带血单核细胞(MNCs)和间充质干细胞,两者都是细胞外微颗粒(MPs)的极佳来源。细胞外微颗粒是一种细胞通讯介质,根据其来源不同,可参与活性氧的形成或解毒。氧化应激通过触发细胞凋亡机制,在癌症的发展和治疗过程中发挥着至关重要的作用,而 CD34+ 细胞与此有牵连。这项工作的目的是研究 HL-60 细胞和从脐带血(UCB)中分离出来的单核细胞在与 CD34 + 微颗粒(CD34 + MPs)接触 24 小时和 48 小时后的氧化应激状态和细胞凋亡情况。超氧化物歧化酶、谷胱甘肽还原酶和谷胱甘肽 S 转移酶的活性以及细胞中的脂质过氧化反应被用作氧化应激标记。将白血病细胞和单核细胞暴露于 CD34 + MPs 24 小时和 48 小时后,两种细胞的抗氧化活性和脂质过氧化作用均有统计学意义的显著增加。此外,CD34 + MPs 会影响 BCL2 和 FAS 及相关蛋白的表达,并下调 HL-60 和单核细胞的造血分化程序。我们的研究结果表明,MPs 通过激活同种和非同种细胞中的抗氧化酶,可作为优化和增强移植的一种手段。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Molecular and biochemical evaluation of oxidative effects of cord blood CD34+ MPs on hematopoietic cells

A graft source for allogeneic hematopoietic stem cell transplantation is umbilical cord blood, which contains umbilical cord blood mononuclear cells (MNCs and mesenchymal stem cells, both an excellent source of extracellular microparticles (MPs). MPs act as cell communication mediators, which are implicated in reactive oxygen species formation or detoxification depending on their origin. Oxidative stress plays a crucial role in both the development of cancer and its treatment by triggering apoptotic mechanisms, in which CD34+ cells are implicated. The aim of this work is to investigate the oxidative stress status and the apoptosis of HL-60 and mononuclear cells isolated from umbilical cord blood (UCB) following a 24- and 48-hour exposure to CD34 + microparticles (CD34 + MPs). The activity of superoxide dismutase, glutathione reductase, and glutathione S-transferase, as well as lipid peroxidation in the cells, were employed as oxidative stress markers. A 24- and 48-hour exposure of leukemic and mononuclear cells to CD34 + -MPs resulted in a statistically significant increase in the antioxidant activity and lipid peroxidation in both cells types. Moreover, CD34 + MPs affect the expression of BCL2 and FAS and related proteins and downregulate the hematopoietic differentiation program in both HL-60 and mononuclear cells. Our results indicate that MPs through activation of antioxidant enzymes in both homozygous and nonhomozygous cells might serve as a means for graft optimization and enhancement.

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来源期刊
CiteScore
4.90
自引率
0.00%
发文量
42
审稿时长
14 days
期刊介绍: Blood Cells, Molecules & Diseases emphasizes not only blood cells, but also covers the molecular basis of hematologic disease and studies of the diseases themselves. This is an invaluable resource to all those interested in the study of hematology, cell biology, immunology, and human genetics.
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