从牛奶中提纯 α-乳白蛋白和 β-乳球蛋白。

IF 1.4 4区生物学 Q4 BIOCHEMICAL RESEARCH METHODS

Protein expression and purification Pub Date : 2024-07-14 DOI:10.1016/j.pep.2024.106555

Kimia Ahadi-Amandi , Seyyed Abolghasem Ghadami , Narges Sayari , Reza Khodarahmi

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引用次数: 0

摘要

乳清是乳制品加工过程中产生的宝贵副产品，含有人体必需的蛋白质，如β-乳球蛋白（βLG）和α-乳白蛋白（αLA），因其营养价值而成为研究的重点。蛋白质提取采用各种技术，包括色谱法和膜过滤法，通常需要多个纯化步骤。在水性两相体系中使用聚乙二醇（PEG）来纯化和浓缩蛋白质（包括乳清中的蛋白质）的方法日益受到重视。我们的研究通过单独使用 PEG 来纯化乳清蛋白，简化了这一过程。这种方法取得了令人瞩目的成果，βLG 的纯度达到 92%，αLA 的纯度达到 90%。这些发现强调了基于 PEG 的纯化技术在分离高纯度乳清蛋白方面的有效性。

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Purification of α-lactalbumin and β-lactoglobulin from cow milk

Whey, a valuable byproduct of dairy processing, contains essential proteins like β-lactoglobulin (βLG) and α-lactalbumin (αLA), making it a focus of research for its nutritional benefits. Various techniques, including chromatography and membrane filtration, are employed for protein extraction, often requiring multiple purification steps. One approach that has gained prominence for the purification and concentration of proteins, including those present in whey, is the use of polyethylene glycol (PEG) in aqueous two-phase systems. Our study simplifies this process by using PEG alone for whey protein purification. This approach yielded impressive results, achieving 92 % purity for βLG and 90 % for αLA. These findings underscore the effectiveness of PEG-based purification in isolating whey proteins with high purity.

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来源期刊

Protein expression and purification 生物-生化研究方法

CiteScore

3.70

自引率

6.20%

发文量

120

审稿时长

32 days

期刊介绍： Protein Expression and Purification is an international journal providing a forum for the dissemination of new information on protein expression, extraction, purification, characterization, and/or applications using conventional biochemical and/or modern molecular biological approaches and methods, which are of broad interest to the field. The journal does not typically publish repetitive examples of protein expression and purification involving standard, well-established, methods. However, exceptions might include studies on important and/or difficult to express and/or purify proteins and/or studies that include extensive protein characterization, which provide new, previously unpublished information.