证明氧−是鲁米诺高量子产率发光的关键中间体

E.Ka. Miller, I. Fridovich
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引用次数: 21

摘要

鲁米诺的化学发光,由于它与碱性H2O2反应,被超氧化物歧化酶或羟基自由基清除剂抑制。血红蛋白显著增强h2o2诱导的鲁米诺发光,降低但不消除对这些抑制剂的敏感性。提出了反应机制来解释这些结果。由于鲁米诺发光依赖于鲁米诺自由基和O2−之间的反应,并且由于鲁米诺自由基可以将二氧还原为O2−,因此超氧化物歧化酶抑制的鲁米诺发光不能可靠地用作O2−产生的检测器。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
A demonstration that o2− is a crucial intermediate in the high quantum yield luminescence of luminol

The chemiluminescence of luminol, due to its reaction with alkaline H2O2, is inhibited by Superoxide dismutase or by hydroxyl radical scavengers. Hematin markedly enhances this H2O2-induced luminescence of luminol and lessens, but does not eliminate, the sensitivity towards these inhibitors. Reaction mechanisms are proposed to account for these results. Since luminol luminescence depends upon a reaction between the luminol radical and O2, and since the luminol radical can reduce dioxygen to O2, Superoxide dismutase-inhibitable luminol luminescence cannot be reliably used as a detector of O2 production.

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