大鼠导管细胞分化成 Islet-Like 细胞

Q4 Biochemistry, Genetics and Molecular Biology

Methods in molecular biology Pub Date : 2024-07-13 DOI:10.1007/7651_2024_558

Nazli Karimi, Gülbahar Boyuk Ozcan

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引用次数: 0

摘要

再生医学研究如何将胰腺导管细胞转化为功能性胰岛细胞，为糖尿病等疾病提供创新疗法。胰腺导管细胞主要支持胰腺的外分泌功能，在特定条件下可分化成包括胰岛细胞在内的各种细胞类型，为研究和治疗开辟了新途径。概述的方案详细介绍了转化过程，包括导管细胞分化诱导和胰岛素分泌能力评估。其主要目的是解决用于移植的胰岛素分泌细胞短缺的问题，从而推动糖尿病治疗方法的发展。

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Rat Ductal Cell-Derived Differentiation into Islet-Like Cells.

Regenerative medicine investigates the conversion of pancreatic ductal cells into functional islet cells, offering innovative treatments for conditions such as diabetes. Ductal cells, primarily supporting the pancreas' exocrine functions, can differentiate into various cell types, including islet cells, under specific conditions, opening new avenues in research and therapy. The outlined protocol elaborates on the conversion process, covering ductal cell differentiation induction, and insulin-producing capacity assessment. The primary objective is to address the shortage of insulin-secreting cells for transplantation, thereby advancing diabetes treatment methodologies.

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来源期刊

Methods in molecular biology Biochemistry, Genetics and Molecular Biology-Genetics

CiteScore

2.00

自引率

0.00%

发文量

3536

期刊介绍： For over 20 years, biological scientists have come to rely on the research protocols and methodologies in the critically acclaimed Methods in Molecular Biology series. The series was the first to introduce the step-by-step protocols approach that has become the standard in all biomedical protocol publishing. Each protocol is provided in readily-reproducible step-by-step fashion, opening with an introductory overview, a list of the materials and reagents needed to complete the experiment, and followed by a detailed procedure that is supported with a helpful notes section offering tips and tricks of the trade as well as troubleshooting advice.