木贼热休克蛋白基因超家族的全基因组鉴定和表达分析

IF 4.6 Q2 MATERIALS SCIENCE, BIOMATERIALS
Xiaozhuo Zong, Yabei Xu, Jing Tao
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引用次数: 0

摘要

Hylurgus ligniperda 属于鞘翅目 Hylurgus Latreille 科。它主要危害松科植物的基部和树干根部。在 42 °C 温度条件下进行短期处理会对木质部瘤蚜造成损害;因此,温度是限制其传播的一个重要因素。热休克蛋白(HSPs)可以保护、清除和修复蛋白质,帮助木贼抵抗高温。然而,有关木贼中 HSP 基因的信息仍然有限。在这项研究中,我们将木质素猪作为研究重点,在全基因组水平上鉴定了 56 个 HligHSP 基因。这些基因被映射到细胞质或细胞核中。一个相同的亚家族表现出极为相似的保守结构域分布。结合之前研究中收集的转录组数据,我们筛选出了六个候选基因,即在不同高温处理过程中特异表达的 HligsHSP-3、HligsHSP-4、HligHSP60-16、HligHSP70-3、HligHSP70-4 和 HligHSP90-1。通过定量聚合酶链反应测定了这六种 HligHSP 在七种温度处理条件下的表达情况。这些基因可能参与了成人的耐热机制。我们的研究结果为进一步研究木犀草履虫的耐热机制奠定了基础。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Genome-wide identification and expression analysis of the heat shock protein gene superfamily in Hylurgus ligniperda

Genome-wide identification and expression analysis of the heat shock protein gene superfamily in Hylurgus ligniperda

Hylurgus ligniperda belongs to Hylurgus Latreille, Curculionidae, Coleoptera. It primarily damages the base and roots of the trunk of pine plants. Short-term treatment at 42 °C can damage Hylurgus ligniperda; therefore, temperature is a vital factor limiting its spread. Heat shock proteins (HSPs) can protect, remove, and repair proteins to help H. ligniperda withstand high temperatures. However, information on HSP genes in H. ligniperda remains limited. In the study, we considered H. ligniperda as the focus of research and identified 56 HligHSP genes at the genome-wide level. These genes were mapped to the cytoplasm or nucleus. An identical subfamily exhibited a closely similar distribution of conserved domains. Combined with the transcriptome data collected in previous studies, we screened six candidate genes, namely HligsHSP-3, HligsHSP-4, HligHSP60-16, HligHSP70-3, HligHSP70-4, and HligHSP90-1, which are specifically expressed during different high-temperature treatments. A quantitative polymerase chain reaction was performed to measure the expression of these six HligHSPs in seven temperature treatment conditions. These genes may be involved in the heat resistance mechanism in adults. Our findings provided a foundation for further studying the heat resistance mechanism in H. ligniperda.

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来源期刊
ACS Applied Bio Materials
ACS Applied Bio Materials Chemistry-Chemistry (all)
CiteScore
9.40
自引率
2.10%
发文量
464
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