肉鸡体内益生菌和预防性药物对免疫相关器官基因表达的调节作用

IF 2 3区 生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Journal of Applied Genetics Pub Date : 2025-02-01 Epub Date: 2024-07-11 DOI:10.1007/s13353-024-00891-y
Ramesha N Wishna-Kadawarage, Katarzyna Połtowicz, Rita M Hickey, Maria Siwek
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引用次数: 0

摘要

作为家禽生产中使用抗生素的一种替代方法,人们一直在对卵内刺激进行深入研究。我们研究了益生菌与植物生物制剂结合使用作为体内刺激的预防性生物制剂的可能性,并报告了其对肉鸡肠道微生物群的有益影响。目前的研究进一步调查了这些鸡免疫相关器官的基因表达,以了解治疗对特定组织的免疫调节作用。在 ROSS308 鸡孵化的第 12 天,将所选的预防性益生菌(大蒜水提取物的中肠白色念珠菌)和单独的益生菌成分注射到 ROSS308 鸡蛋中,并使用 qPCR 方法测定 35 日龄时盲肠扁桃体、脾脏和肝脏中的基因表达。将各处理的相对表达量与阳性对照(注射生理盐水)进行比较。结果显示,益生菌组的盲肠扁桃体和预防用药组的肝脏中的促炎和抗炎细胞因子下调。两组的脾脏均显示 AVBD1 上调,益生菌组显示 IL1-β 上调。益生菌组肝脏中与能量代谢(COX16)、蛋白质代谢(mTOR)和脂类代谢(CYP46A1)相关的基因表达增加,而预防菌组肝脏中与胆固醇合成(SREBP1)和葡萄糖运输(SLC2A2)相关的基因表达减少。总之,在卵内给药大蒜水提取物的情况下,白色念珠菌会对鸡体内的基因表达产生不同程度的调节作用。为了优化肉鸡的益处,还需要对不同的预防性药物组合进行进一步的体内研究。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Modulation of gene expression in immune-related organs by in ovo stimulation with probiotics and prophybiotics in broiler chickens.

Modulation of gene expression in immune-related organs by in ovo stimulation with probiotics and prophybiotics in broiler chickens.

In ovo stimulation has been studied intensively as an alternative to antibiotic use in poultry production. We investigated the potential use of a probiotic in combination with a phytobiotic as a prophybiotic for in ovo stimulation and reported its beneficial effects on the gut microbiome of broiler chickens. The current study further investigates the gene expression in the immune-related organs of these chickens to understand the tissue-specific immunomodulatory effects of the treatments. The selected prophybiotic (Leuconostoc mesenteroides with garlic aqueous extract) and its probiotic component alone were injected into ROSS308 chicken eggs on the 12th day of incubation, and gene expression in cecal tonsils, spleen, and liver at 35 days of age was determined using qPCR method. The relative expression of each treatment was compared to the positive control, chickens injected with physiological saline in ovo. The results displayed a downregulation of pro- and anti-inflammatory cytokines in the cecal tonsils of the probiotic group and the liver of the prophybiotic group. The spleen displayed upregulated AVBD1 in both groups and upregulated IL1-β in the probiotic group. The probiotic group displayed increased expression of genes related to metabolism of energy (COX16), protein (mTOR), and lipids (CYP46A1) whereas the prophybiotic group displayed reduced expression of genes related to cholesterol synthesis (SREBP1) and glucose transportation (SLC2A2) in the liver. In conclusion, Leuconostoc mesenteroides differentially modulated gene expression in chickens when administered in ovo in combination with garlic aqueous extract. Further in ovo studies with different prophybiotic combinations are required to optimize the benefits in broiler chickens.

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来源期刊
Journal of Applied Genetics
Journal of Applied Genetics 生物-生物工程与应用微生物
CiteScore
4.30
自引率
4.20%
发文量
62
审稿时长
6-12 weeks
期刊介绍: The Journal of Applied Genetics is an international journal on genetics and genomics. It publishes peer-reviewed original papers, short communications (including case reports) and review articles focused on the research of applicative aspects of plant, human, animal and microbial genetics and genomics.
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