利用脂肪干细胞构建三维组织,用于骨缺损愈合:股骨头体外研究。

IF 3.2 3区 生物学 Q2 BIOCHEMICAL RESEARCH METHODS
Aditi Mahajan, Siddhartha Sharma, Sanjay Kumar Bhadada, Aditya Aggarwal, Shalmoli Bhattacharyya
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引用次数: 0

摘要

骨移植替代物(BGS)与间充质干细胞(MSCs)的相容性是用于修复骨缺损时需要考虑的一个重要参数,因为它最终会影响临床效果。本研究筛选了几种市售 BGS - β-磷酸三钙(β-TCP)、硫酸钙、海绵明胶和不同形式的羟基磷灰石(HAP),以了解它们与来自脂肪组织的间充质干细胞(ADSCs)的相互作用。结果表明,与其他支架相比,羟基磷灰石块能更好地支持 ADSC 的活力、形态、迁移和分化。这些结果有力地说明了对骨支架的细胞兼容性进行临床前评估的重要性。此外,在使用患者骨小梁外植体制作的体外骨缺损模型中,对含有 ADSCs 的 HAP 块的骨再生潜力进行了评估。这些外植体在体外培养了 45 天,并通过成骨基因的表达、ALP 分泌和高分辨率计算机断层扫描评估了骨形成情况。我们的研究结果证实,在体外环境中,骨修复过程非常活跃。添加 ADSCs 能明显加速修复过程并改善骨的微结构。这种体外骨缺损模型可作为动物实验的一种可行替代方法,也是在受控条件下评估患者特定骨疗法的一种有效工具。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Engineering a 3-dimensional tissue construct with adipose-derived stem cells for healing bone defect: An ex vivo study with femur head

Engineering a 3-dimensional tissue construct with adipose-derived stem cells for healing bone defect: An ex vivo study with femur head

The compatibility of bone graft substitutes (BGS) with mesenchymal stem cells (MSCs) is an important parameter to consider for their use in repairing bone defects as it eventually affects the clinical outcome. In the present study, a few commercially available BGS – β-tricalcium phosphate (β-TCP), calcium sulfate, gelatin sponge, and different forms of hydroxyapatite (HAP) were screened for their interactions with MSCs from adipose tissue (ADSCs). It was demonstrated that HAP block favorably supported ADSC viability, morphology, migration, and differentiation compared to other scaffolds. The results strongly suggest the importance of preclinical evaluation of bone scaffolds for their cellular compatibility. Furthermore, the bone regenerative potential of HAP block with ADSCs was evaluated in an ex vivo bone defect model developed using patient derived trabecular bone explants. The explants were cultured for 45 days in vitro and bone formation was assessed by expression of osteogenic genes, ALP secretion, and high resolution computed tomography. Our findings confirmed active bone repair process in ex vivo settings. Addition of ADSCs significantly accelerated the repair process and improved bone microarchitecture. This ex vivo bone defect model can emerge as a viable alternative to animal experimentation and also as a potent tool to evaluate patient specific bone therapeutics under controlled conditions.

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来源期刊
Biotechnology Journal
Biotechnology Journal Biochemistry, Genetics and Molecular Biology-Molecular Medicine
CiteScore
8.90
自引率
2.10%
发文量
123
审稿时长
1.5 months
期刊介绍: Biotechnology Journal (2019 Journal Citation Reports: 3.543) is fully comprehensive in its scope and publishes strictly peer-reviewed papers covering novel aspects and methods in all areas of biotechnology. Some issues are devoted to a special topic, providing the latest information on the most crucial areas of research and technological advances. In addition to these special issues, the journal welcomes unsolicited submissions for primary research articles, such as Research Articles, Rapid Communications and Biotech Methods. BTJ also welcomes proposals of Review Articles - please send in a brief outline of the article and the senior author''s CV to the editorial office. BTJ promotes a special emphasis on: Systems Biotechnology Synthetic Biology and Metabolic Engineering Nanobiotechnology and Biomaterials Tissue engineering, Regenerative Medicine and Stem cells Gene Editing, Gene therapy and Immunotherapy Omics technologies Industrial Biotechnology, Biopharmaceuticals and Biocatalysis Bioprocess engineering and Downstream processing Plant Biotechnology Biosafety, Biotech Ethics, Science Communication Methods and Advances.
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