枳壳果皮精油的代谢谱分析和酶抑制活性

2区 医学 Q1 Medicine
Naglaa S. Ashmawy, Nilofar Nilofar, Gokhan Zengin, Omayma A. Eldahshan
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引用次数: 0

摘要

苦橙(Citrus aurantium)是一种果实灌木,原产于世界各地的热带和亚热带国家,因其营养保健价值而在许多地区种植。本研究通过三种不同的提取方法,研究了从埃及栽培的苦橙果皮中提取的挥发性成分的代谢谱分析和酶抑制活性。本研究采用蒸汽蒸馏(SD)、水蒸馏(HD)和微波辅助水蒸馏(MAHD)三种方法分离了枳壳果皮中的挥发性化学成分,然后用气相色谱-质谱(GC-MS)对其进行了研究。通过 DPPH、ABTS、FRAP、CUPRAC、磷钼和金属螯合潜力等不同检测方法对其抗氧化潜力进行了评估。此外,还使用 BChE、AChE、酪氨酸酶、葡萄糖苷酶和淀粉酶检测了三种精油的酶抑制效果。通过气相色谱/质谱分析,共检测到六种化合物。三种提取方法得到的主要成分都是柠檬烯(SD 提取率为 98.86%,HD 提取率为 98.68%,MAHD 提取率为 99.23%)。三种油的化合物组成存在差异。水蒸馏技术产生的化合物数量最多,尤其是两种含氧单萜烯:芳樟醇(0.12%)和α-松油醇乙酸酯(0.1%)。在我们的研究中,枳壳皮精油提取方法的不同导致了精油化学成分的不同。柑橘精油及其成分具有潜在的抗氧化、抗胆碱酯酶、抗黑色素生成和抗糖尿病活性。芳樟醇和α-松油醇乙酸酯的存在可能是通过 HD 分离出的精油在自由基清除和 AChE 抑制试验以及酶抑制试验中具有更高活性的原因。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Metabolic profiling and enzyme inhibitory activity of the essential oil of citrus aurantium fruit peel
Bitter orange (Citrus aurantium) is a fruiting shrub native to tropical and subtropical countries around the world and cultivated in many regions due to its nutraceutical value. The current study investigated the metabolic profiling and enzyme inhibitory activities of volatile constituents derived from the C. aurantium peel cultivated in Egypt by three different extraction methods. The volatile chemical constituents of the peel of C. aurantium were isolated using three methods; steam distillation (SD), hydrodistillation (HD), and microwave-assisted hydrodistillation (MAHD), and then were investigated by GC-MS. The antioxidant potential was evaluated by different assays such as DPPH, ABTS, FRAP, CUPRAC, and phosphomolybdenum and metal chelating potential. Moreover, the effect of enzyme inhibition of the three essential oils was tested using BChE, AChE, tyrosinase, glucosidase, as well as amylase assays. A total of six compounds were detected by GC/MS analysis. The major constituent obtained by all three extraction methods was limonene (98.86% by SD, 98.68% by HD, and 99.23% by MAHD). Differences in the composition of the compounds of the three oils were observed. The hydrodistillation technique has yielded the highest number of compounds, notably two oxygenated monoterpenes: linalool (0.12%) and α-terpineol acetate (0.1%). In our study differences in the extraction methods of C. aurantium peel oils resulted in differences in the oils’ chemical composition. Citrus essential oils and their components showed potential antioxidant, anticholinesterase, antimelanogenesis, and antidiabetic activities. The presence of linalool and α-terpineol acetate may explain the superior activity observed for the oil isolated by HD in both radical scavenging and AChE inhibition assays, as well as in the enzyme inhibition assays.
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来源期刊
BMC Complementary and Alternative Medicine
BMC Complementary and Alternative Medicine INTEGRATIVE & COMPLEMENTARY MEDICINE-
CiteScore
7.00
自引率
0.00%
发文量
0
审稿时长
3 months
期刊介绍: BMC Complementary Medicine and Therapies is an open access journal publishing original peer-reviewed research articles on interventions and resources that complement or replace conventional therapies, with a specific emphasis on research that explores the biological mechanisms of action, as well as their efficacy, safety, costs, patterns of use and/or implementation.
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