运动通过抑制 RhoA/ROCK 信号传导减轻慢性阻塞性肺病小鼠的膈肌萎缩

IF 2.8 3区 医学 Q1 Medicine
Peijun Li, Yingqi Wang, Yuanyuan Cao, Jiacheng Shi, Meiling Jiang, Xiaoyu Han, Linhong Jiang, Yidie Bao, Weibing Wu, Xiaodan Liu
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引用次数: 0

摘要

背景:运动是肺康复不可或缺的组成部分,具有很强的抗炎作用。然而,运动预防 COPD(慢性阻塞性肺病)膈肌萎缩的机制仍不清楚:将 40 只雄性 C57BL/6 小鼠分为对照组(n=16)和烟雾组(n=24)。烟雾组小鼠暴露于香烟烟雾(CS)中六个月。然后将小鼠分为模型组和运动训练组,每组 2 个月。观察肺部和膈肌的组织学变化。随后,RhoA/ROCK 的激动剂 U46639 和拮抗剂 Y27632 在经 LPS 处理的 C2C12 肌细胞中进行机械拉伸。通过 Western 印迹法测定了 Atrogin-1、MuRF-1、MyoD、Myf5、IL-1β、TNF-α 和 RhoA/ROCK 的表达水平:结果:在 COPD 小鼠中观察到膈肌萎缩和 RhoA/ROCK 表达增加。运动训练减轻了 COPD 小鼠横膈膜的萎缩,降低了 MuRF-1 的表达,增加了 MyoD 的表达。运动还会影响 RhoA/ROCK 和炎症相关蛋白的上调。在 C2C12 肌母细胞的体外实验中,LPS 显著增加了炎症和蛋白质降解的水平,而 Y27632 或与机械拉伸相结合则大大阻止了这一现象的发生:结论:RhoA/ROCK在预防慢性阻塞性肺病患者膈肌萎缩方面发挥着重要作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Exercise Attenuate Diaphragm Atrophy in COPD Mice via Inhibiting the RhoA/ROCK Signaling
Background: Exercise is an indispensable component of pulmonary rehabilitation with strong anti-inflammatory effects. However, the mechanisms by which exercise prevents diaphragmatic atrophy in COPD (chronic obstructive pulmonary disease) remain unclear.
Methods: Forty male C57BL/6 mice were assigned to the control (n=16) and smoke (n=24) groups. Mice in the smoke group were exposed to the cigarette smoke (CS) for six months. They were then divided into model and exercise training groups for 2 months. Histological changes were observed in lung and diaphragms. Subsequently, agonist U46639 and antagonist Y27632 of RhoA/ROCK were subjected to mechanical stretching in LPS-treated C2C12 myoblasts. The expression levels of Atrogin-1, MuRF-1, MyoD, Myf5, IL-1β, TNF-α, and RhoA/ROCK were determined by Western blotting.
Results: Diaphragmatic atrophy and increased RhoA/ROCK expression were observed in COPD mice. Exercise training attenuated diaphragmatic atrophy, decreased the expression of MuRF-1, and increased MyoD expression in COPD diaphragms. Exercise also affects the upregulation of RhoA/ROCK and inflammation-related proteins. In in vitro experiments with C2C12 myoblasts, LPS remarkably increased the level of inflammation and protein degradation, whereas Y27632 or combined with mechanical stretching prevented this phenomenon considerably.
Conclusion: RhoA/ROCK plays an important role in the prevention of diaphragmatic atrophy in COPD.

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来源期刊
CiteScore
5.10
自引率
10.70%
发文量
372
审稿时长
16 weeks
期刊介绍: An international, peer-reviewed journal of therapeutics and pharmacology focusing on concise rapid reporting of clinical studies and reviews in COPD. Special focus will be given to the pathophysiological processes underlying the disease, intervention programs, patient focused education, and self management protocols. This journal is directed at specialists and healthcare professionals
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