慢性不可预测轻度应激对雄性小鼠肠道感觉和功能的影响

IF 2.6 4区 心理学 Q2 BEHAVIORAL SCIENCES
Fangyuan Liang, Suzhen Liu, Heng Zhang, Ronglan Xiang, Mengting Xie, Xiaoru He, Sunyi Wang, Song Wu, Jia Li
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引用次数: 0

摘要

压力与肠易激综合征(IBS)的发生有关,人们探索了多种方法来模拟肠易激综合征与其他刺激的结合。然而,单独的压力是否能诱发动物肠易激综合征仍不清楚。本研究旨在调查慢性不可预测轻度应激(CUMS)对小鼠胃肠道感觉和功能的影响,并评估CUMS作为IBS模型方法的潜力。为了评估小鼠的行为,我们对小鼠进行了开阔地试验、蔗糖偏好试验和称重,结果显示CUMS确实诱发了小鼠的焦虑和抑郁,并导致体重下降。粪便分析、全胃肠道运输试验和结肠推进试验等进一步分析表明,CUMS导致小鼠排便异常和胃肠道运动紊乱。此外,腹部退缩反射试验表明,接触 CUMS 的小鼠内脏敏感性增加。使用苏木精和伊红染色法进行的组织学检查显示,暴露于 CUMS 的小鼠结肠没有明显的组织学改变,但确实出现了轻微的炎症细胞浸润。总之,研究结果表明,CUMS 可以在小鼠体内复制类似肠易激综合征的症状,为肠易激综合征的建模提供了一种新的自上而下的方法。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Effects of chronic unpredictable mild stress on gut sensation and function in male mice.

Stress has been linked to the development of irritable bowel syndrome (IBS), and various methods have been explored to model IBS in combination with other stimuli. However, it remains unclear whether stress alone can induce IBS in animals. This study aimed to investigate the impact of chronic unpredictable mild stress (CUMS) on gastrointestinal sensation and function in mice and assess the potential of CUMS as a modeling approach for IBS. To evaluate the mice's behavior, we conducted open field test, sucrose preference test and weighed the mice, revealing that CUMS indeed induced anxiety and depression in the mice and caused weight loss. Further analyses, including fecal analysis, a total gastrointestinal transport test, and a colon propulsion test, demonstrated that CUMS led to abnormal defecation and disruptions in gastrointestinal motility in the mice. Additionally, the abdominal withdrawal reflex test indicated an increase in visceral sensitivity in CUMS-exposed mice. Histological examination using hematoxylin and eosin staining revealed no significant histological alterations in the colons of CUMS-exposed mice, but it did show a minor degree of inflammatory cell infiltration. In summary, the findings suggest that CUMS can replicate IBS-like symptoms in mice, offering a novel top-down approach to modeling IBS.

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来源期刊
CiteScore
5.60
自引率
0.00%
发文量
25
审稿时长
6-12 weeks
期刊介绍: The journal Stress aims to provide scientists involved in stress research with the possibility of reading a more integrated view of the field. Peer reviewed papers, invited reviews and short communications will deal with interdisciplinary aspects of stress in terms of: the mechanisms of stressful stimulation, including within and between individuals; the physiological and behavioural responses to stress, and their regulation, in both the short and long term; adaptive mechanisms, coping strategies and the pathological consequences of stress. Stress will publish the latest developments in physiology, neurobiology, molecular biology, genetics research, immunology, and behavioural studies as they impact on the understanding of stress and its adverse consequences and their amelioration. Specific approaches may include transgenic/knockout animals, developmental/programming studies, electrophysiology, histochemistry, neurochemistry, neuropharmacology, neuroanatomy, neuroimaging, endocrinology, autonomic physiology, immunology, chronic pain, ethological and other behavioural studies and clinical measures.
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