Hooman Shirvani, Ali Ashraf Mehrabi, Mohsen Farshadfar, Hooshmand Safari, Ali Arminian, Foad Fatehi
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Additionally, the nutritional characteristics of the straw produced by each genotype of this germplasm were evaluated under rainfed and irrigated conditions, including crude protein (%CP), crude fiber (%CF), acid detergent fiber (%ADF), dry matter digestibility (%DMD), metabolism energy (ME), digestible energy (DE), and total digestible nutrient (TNT) characteristics. The genetic structure of the population was assessed using 97 different alleles amplified for 35 simple sequence repeat (SSR) and expressed sequence tag (EST)-derived SSR markers. The analysis revealed the presence of seven distinct sub-populations within this diverse panel of wild barley. Marker-trait association analysis identified the markers BeMAG0323 and GBM1461 as highly informative loci for the seed quality traits under both conditions. The markers SCSSR15864 and BMAC0154 were found to have the most significant association with the quality characteristics of the straw. This study is the first to establish a connection between straw traits and seed quality in barley by utilizing SSR and EST-SSR markers. By introducing new plant material that has been thoroughly studied for its economic qualitative characteristics in terms of seed and straw of barley, this research serves as a pre-breeding effort to enhance the quality of barley cultivars and to utilize the informative markers introduced here for future genomic selection programs.</p>","PeriodicalId":12467,"journal":{"name":"Genetic Resources and Crop Evolution","volume":null,"pages":null},"PeriodicalIF":1.6000,"publicationDate":"2024-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Phenotyping variability and detecting informative SSR and EST-SSR markers for malt and straw quality characteristics in a diverse panel of wild barley (H. spontaneum) under rainfed and irrigated conditions\",\"authors\":\"Hooman Shirvani, Ali Ashraf Mehrabi, Mohsen Farshadfar, Hooshmand Safari, Ali Arminian, Foad Fatehi\",\"doi\":\"10.1007/s10722-024-02062-8\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><i>Hordeum spontaneum</i> Koch, the progenitor of cultivated barley, shares the same number of chromosomes as cultivated barley and can be easily crossed without any biological barriers. 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引用次数: 0
摘要
自生大麦(Hordeum spontaneum Koch)是栽培大麦的祖先,与栽培大麦具有相同数量的染色体,可以很容易地进行杂交,没有任何生物障碍。本研究侧重于从伊朗扎格罗斯盆地的自然栖息地采集的 H. spontaneum。根据各种参数,如发芽率、冷水和热水提取物、糖度、还原糖和颜色强度等,对种子质量进行了分析。此外,还评估了该种质的每个基因型在雨养和灌溉条件下生产的秸秆的营养特性,包括粗蛋白(%CP)、粗纤维(%CF)、酸性洗涤纤维(%ADF)、干物质消化率(%DMD)、代谢能(ME)、可消化能(DE)和可消化总养分(TNT)特性。通过对 35 个简单序列重复(SSR)和表达序列标记(EST)衍生的 SSR 标记扩增出的 97 个不同等位基因,对种群的遗传结构进行了评估。分析表明,在这个多样化的野生大麦群体中存在七个不同的亚群。标记-性状关联分析发现,标记 BeMAG0323 和 GBM1461 是两种条件下种子质量性状的高信息位点。研究发现,标记物 SCSSR15864 和 BMAC0154 与稻草质量特征的关联最为显著。本研究首次利用 SSR 和 EST-SSR 标记建立了大麦秸秆性状与种子质量之间的联系。通过引入新的植物材料,并对其在大麦种子和秸秆方面的经济质量特性进行深入研究,该研究可作为育种前的一项工作,以提高大麦栽培品种的质量,并将此处引入的信息标记用于未来的基因组选育计划。
Phenotyping variability and detecting informative SSR and EST-SSR markers for malt and straw quality characteristics in a diverse panel of wild barley (H. spontaneum) under rainfed and irrigated conditions
Hordeum spontaneum Koch, the progenitor of cultivated barley, shares the same number of chromosomes as cultivated barley and can be easily crossed without any biological barriers. This study focuses on a collection of H. spontaneum obtained from natural habitats in the Zagros basin, Iran. This collection demonstrates a wide range of variations in seed quality, which were analyzed based on various parameters such as malting efficiency, cold and hot water extract, brix, reducing sugar, and color intensity. Additionally, the nutritional characteristics of the straw produced by each genotype of this germplasm were evaluated under rainfed and irrigated conditions, including crude protein (%CP), crude fiber (%CF), acid detergent fiber (%ADF), dry matter digestibility (%DMD), metabolism energy (ME), digestible energy (DE), and total digestible nutrient (TNT) characteristics. The genetic structure of the population was assessed using 97 different alleles amplified for 35 simple sequence repeat (SSR) and expressed sequence tag (EST)-derived SSR markers. The analysis revealed the presence of seven distinct sub-populations within this diverse panel of wild barley. Marker-trait association analysis identified the markers BeMAG0323 and GBM1461 as highly informative loci for the seed quality traits under both conditions. The markers SCSSR15864 and BMAC0154 were found to have the most significant association with the quality characteristics of the straw. This study is the first to establish a connection between straw traits and seed quality in barley by utilizing SSR and EST-SSR markers. By introducing new plant material that has been thoroughly studied for its economic qualitative characteristics in terms of seed and straw of barley, this research serves as a pre-breeding effort to enhance the quality of barley cultivars and to utilize the informative markers introduced here for future genomic selection programs.
期刊介绍:
Genetic Resources and Crop Evolution is devoted to all aspects of plant genetic resources research. It publishes original articles in the fields of taxonomical, morphological, physiological, biochemical, genetical, cytological or ethnobotanical research of genetic resources and includes contributions to gene-bank management in a broad sense, that means to collecting, maintenance, evaluation, storage and documentation.
Areas of particular interest include:
-crop evolution
-domestication
-crop-weed relationships
-related wild species
-history of cultivated plants including palaeoethnobotany.
Genetic Resources and Crop Evolution also publishes short communications, e.g. newly described crop taxa, nomenclatural notes, reports of collecting missions, evaluation results of gene-bank material etc. as well as book reviews of important publications in the field of genetic resources.
Every volume will contain some review articles on actual problems. The journal is the internationalized continuation of the German periodical Die Kulturpflanze, published formerly by the Institute of Plant Genetics and Crop Plant Research at Gatersleben, Germany.
All contributions are in the English language and are subject to peer reviewing.