{"title":"内部和外部光谱光转换放大杜纳藻的生长/生物产品的形成","authors":"Mojgan Mohebi Najafabadi, Fereshteh Naeimpoor","doi":"10.1007/s10811-024-03304-z","DOIUrl":null,"url":null,"abstract":"<p>Rhodamine-6-G (R6G) was used to investigate the effect of internal/external light conversion on <i>Dunaliella salina</i> in a 1000 mL double-layer flat plate photobioreactor (DLF-PBR) and a 900 mL cylindrical photobioreactor (C-PBR). External spectrum conversion was examined in the DLF-PBR where the outer layer was filled with four different dye solutions (water/ethanol as solvents, dye at 25/50 ppm) to select the appropriate dye level and solvent type. The highest biomass (2.1 g L<sup>-1</sup>) and productivity (116 mg L<sup>-1</sup>day<sup>-1</sup>) were obtained at low dye level in ethanol, while the highest productivities of storage materials (lipid:7.5, starch:18.73 mg L<sup>-1</sup>day<sup>-1</sup>) belonged to high level of dye in water. To benefit from conventional bioreactors with lower cost, internal conversion was carried out in C-PBR by addition of dye into medium aiming to compare growth and product formation with/without spectrum conversion. The highest level of biomass (3.49 g L<sup>-1</sup>) was achieved by light conversion in C-PBR. Additionally, the effect of adaption of cells to dye was investigated in the C-PBR. A long lag-phase was observed in the case of non-adapted cells while this considerably reduced in the case of adapted cells. Moreover, a two-stage cultivation was studied in the C-PBR in which mixed red-blue LEDs exposure allowing enhanced growth without dye was used in the 1<sup>st</sup>-stage and this was followed by addition of R6G and shifting to fluorescent exposure in the 2<sup>nd</sup>-stage, which resulted in 146 and 293% higher productivities of lipid and starch, respectively, compared to control. 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引用次数: 0
摘要
在 1000 mL 双层平板光生物反应器(DLF-PBR)和 900 mL 圆柱形光生物反应器(C-PBR)中,使用罗丹明-6-G(R6G)研究了内部/外部光转换对盐生杜纳藻的影响。在 DLF-PBR 中,外层填充了四种不同的染料溶液(以水/乙醇为溶剂,染料浓度为 25/50 ppm),以选择合适的染料浓度和溶剂类型。在乙醇中使用低浓度染料时,生物量(2.1 克/升-1)和生产率(116 毫克/升-1 天-1)最高;而在水中使用高浓度染料时,贮存物质的生产率(脂质:7.5,淀粉:18.73 毫克/升-1 天-1)最高。为了从成本较低的传统生物反应器中获益,通过在培养基中添加染料,在 C-PBR 中进行了内部转化,目的是比较有/无光谱转化时的生长和产品形成情况。通过在 C-PBR 中进行光转换,生物量达到了最高水平(3.49 g L-1)。此外,还研究了 C-PBR 中细胞对染料适应性的影响。在未适应的细胞中观察到较长的滞后期,而在适应的细胞中滞后期大大缩短。此外,还对 C-PBR 的两阶段培养进行了研究,其中第一阶段使用红蓝 LED 混合照射,在不使用染料的情况下促进生长,随后在第二阶段添加 R6G 并转为荧光照射,与对照组相比,脂质和淀粉的生产率分别提高了 146% 和 293%。细胞中最高的脂质和淀粉含量(9.91、22.15% g-1DCW)也与这一策略相对应。
Internal and external spectral light conversion amplifying growth/bio-products formation of Dunaliella salina
Rhodamine-6-G (R6G) was used to investigate the effect of internal/external light conversion on Dunaliella salina in a 1000 mL double-layer flat plate photobioreactor (DLF-PBR) and a 900 mL cylindrical photobioreactor (C-PBR). External spectrum conversion was examined in the DLF-PBR where the outer layer was filled with four different dye solutions (water/ethanol as solvents, dye at 25/50 ppm) to select the appropriate dye level and solvent type. The highest biomass (2.1 g L-1) and productivity (116 mg L-1day-1) were obtained at low dye level in ethanol, while the highest productivities of storage materials (lipid:7.5, starch:18.73 mg L-1day-1) belonged to high level of dye in water. To benefit from conventional bioreactors with lower cost, internal conversion was carried out in C-PBR by addition of dye into medium aiming to compare growth and product formation with/without spectrum conversion. The highest level of biomass (3.49 g L-1) was achieved by light conversion in C-PBR. Additionally, the effect of adaption of cells to dye was investigated in the C-PBR. A long lag-phase was observed in the case of non-adapted cells while this considerably reduced in the case of adapted cells. Moreover, a two-stage cultivation was studied in the C-PBR in which mixed red-blue LEDs exposure allowing enhanced growth without dye was used in the 1st-stage and this was followed by addition of R6G and shifting to fluorescent exposure in the 2nd-stage, which resulted in 146 and 293% higher productivities of lipid and starch, respectively, compared to control. The highest cell contents of lipid and starch (9.91, 22.15% g g-1DCW) also corresponded to this strategy.
期刊介绍:
The Journal of Applied Phycology publishes work on the rapidly expanding subject of the commercial use of algae.
The journal accepts submissions on fundamental research, development of techniques and practical applications in such areas as algal and cyanobacterial biotechnology and genetic engineering, tissues culture, culture collections, commercially useful micro-algae and their products, mariculture, algalization and soil fertility, pollution and fouling, monitoring, toxicity tests, toxic compounds, antibiotics and other biologically active compounds.
Each issue of the Journal of Applied Phycology also includes a short section for brief notes and general information on new products, patents and company news.