人类和小鼠肠道神经系统神经元周围网的描述性研究。

IF 4.2 3区 医学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY
Matheus Deroco Veloso da Silva, Cristiano Correia Bacarin, Camila Cristina Alves Machado, Anelise Franciosi, Joana Darc de Lima Mendes, Paulo da Silva Watanabe, Carlos Alberto Miqueloto, Victor Fattori, Orlando Yesid Esparza Albarracin, Waldiceu A. Verri Jr, Rubina Aktar, Madusha Peiris, Qasim Aziz, L. Ashley Blackshaw, Eduardo José de Almeida Araújo
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引用次数: 0

摘要

神经元周围网(PNN)是中枢神经系统(CNS)中特定神经元群周围细胞外基质的高度特化结构。它们具有优化生理过程和保护神经元免受有害刺激的功能。传统上,人们只描述了它们在中枢神经系统中的存在。然而,到目前为止,还没有关于肠神经系统(ENS)中 PNN 的存在和组成的描述。因此,我们的目标是证明肠道神经系统中存在 PNN 并确定其成分的特征。我们通过 RT-PCR 和免疫荧光试验分析了小鼠和人类的肠道组织样本。我们使用一种标记物(紫藤凝集素)作为检测中枢神经系统中是否存在 PNN 的标准,并使用抗体标记中枢神经系统中与 PNN 相关的四个主要蛋白家族的成员。我们的研究结果表明,这两个物种的中枢神经系统中都存在 PNN 的成分;但其分子组成具有物种特异性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Descriptive study of perineuronal net in enteric nervous system of humans and mice

Descriptive study of perineuronal net in enteric nervous system of humans and mice

Perineuronal nets (PNN) are highly specialized structures of the extracellular matrix around specific groups of neurons in the central nervous system (CNS). They play functions related to optimizing physiological processes and protection neurons against harmful stimuli. Traditionally, their existence was only described in the CNS. However, there was no description of the presence and composition of PNN in the enteric nervous system (ENS) until now. Thus, our aim was to demonstrate the presence and characterize the components of the PNN in the enteric nervous system. Samples of intestinal tissue from mice and humans were analyzed by RT-PCR and immunofluorescence assays. We used a marker (Wisteria floribunda agglutinin) considered as standard for detecting the presence of PNN in the CNS and antibodies for labeling members of the four main PNN-related protein families in the CNS. Our results demonstrated the presence of components of PNN in the ENS of both species; however its molecular composition is species-specific.

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来源期刊
Journal of Neurochemistry
Journal of Neurochemistry 医学-神经科学
CiteScore
9.30
自引率
2.10%
发文量
181
审稿时长
2.2 months
期刊介绍: Journal of Neurochemistry focuses on molecular, cellular and biochemical aspects of the nervous system, the pathogenesis of neurological disorders and the development of disease specific biomarkers. It is devoted to the prompt publication of original findings of the highest scientific priority and value that provide novel mechanistic insights, represent a clear advance over previous studies and have the potential to generate exciting future research.
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