Pelin Kılıç, Cansu Özdemir, Begüm Coşar, Büşra Nigar Savran, Aysun Sarıkaya, Begüm Sargon, Alım Toprakkale, İrem Songür, Özlem Kandemir Seçgin, Pınar Akpınar Oktar, Elif NazIı Çetindağ, Deniz Yurtsever Sarıca, Serpil Taşdelen, Üstün Ezer, Ahmet Emin Kürekçi, Günhan Gürman
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Upstream Process Protocol for MSCs Isolated from Different Human-Based Tissue Origins.
This chapter introduces the increasing significance of mesenchymal stromal/stem cell (MSC) production in regenerative medicine and cellular therapeutics, outlines the growing interest in MSCs for various medical applications, and highlights their potential in advanced therapy medicinal products (ATMPs) and the advancements in cell culture technologies that have facilitated large-scale MSC production under Good Manufacturing Practices (GMP), ensuring safety and efficacy. This chapter describes an optimized upstream protocol for laboratory-scale MSC production from different tissue sources. This protocol, conducted in flasks, controls critical parameters and lays the foundation for downstream processing to generate ATMPs. This comprehensive approach underscores the potential of MSCs in clinical applications and the importance of tailored production processes.
期刊介绍：
For over 20 years, biological scientists have come to rely on the research protocols and methodologies in the critically acclaimed Methods in Molecular Biology series. The series was the first to introduce the step-by-step protocols approach that has become the standard in all biomedical protocol publishing. Each protocol is provided in readily-reproducible step-by-step fashion, opening with an introductory overview, a list of the materials and reagents needed to complete the experiment, and followed by a detailed procedure that is supported with a helpful notes section offering tips and tricks of the trade as well as troubleshooting advice.
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