小鼠培养的星形胶质细胞线粒体中脂肪酸的β-氧化偏好。

IF 16.4 1区 化学 Q1 CHEMISTRY, MULTIDISCIPLINARY
Laarni Grace Corales, Hitoshi Inada, Yuji Owada, Noriko Osumi
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引用次数: 0

摘要

大脑利用葡萄糖作为主要的能量底物,但也利用脂肪酸在线粒体中进行β-氧化。据报道,β-氧化主要发生在星形胶质细胞中,但其对不同脂肪酸的能力和功效仍然未知。在这里,我们展示了小鼠培养的星形胶质细胞线粒体中脂肪酸对β-氧化的偏好。使用细胞外通量分析仪进行的脂肪酸氧化分析表明,饱和或单不饱和脂肪酸(棕榈酸和油酸)是比多不饱和脂肪酸(如花生四烯酸和二十二碳六烯酸)更优先的底物。我们还报告说,星形胶质细胞中表达的脂肪酸结合蛋白对脂肪酸运输到线粒体进行β氧化的贡献较小。我们的研究结果有助于了解大脑中通过脂肪酸消耗进行的能量代谢。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Fatty acid preference for beta-oxidation in mitochondria of murine cultured astrocytes

Fatty acid preference for beta-oxidation in mitochondria of murine cultured astrocytes

The brain utilizes glucose as a primary energy substrate but also fatty acids for the β-oxidation in mitochondria. The β-oxidation is reported to occur mainly in astrocytes, but its capacity and efficacy against different fatty acids remain unknown. Here, we show the fatty acid preference for the β-oxidation in mitochondria of murine cultured astrocytes. Fatty acid oxidation assay using an extracellular flux analyzer showed that saturated or monosaturated fatty acids, palmitic acid and oleic acid, are preferred substrates over polyunsaturated fatty acids like arachidonic acid and docosahexaenoic acid. We also report that fatty acid binding proteins expressed in the astrocytes contribute less to fatty acid transport to mitochondria for β-oxidation. Our results could give insight into understanding energy metabolism through fatty acid consumption in the brain.

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来源期刊
Accounts of Chemical Research
Accounts of Chemical Research 化学-化学综合
CiteScore
31.40
自引率
1.10%
发文量
312
审稿时长
2 months
期刊介绍: Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.
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