{"title":"一氧化氮参与马铃薯幼苗在冷胁迫下的生化和生理反应","authors":"Jafar Nabati, Zahra Nemati, Elaheh Boroumand Rezazadeh","doi":"10.1007/s00344-024-11401-z","DOIUrl":null,"url":null,"abstract":"<p>Cold temperatures harm tropical crops, but understanding how molecular signals help plants cope could aid in climate change adaptation. This study tested if sodium nitroprusside (SNP), a nitric oxide (NO) donor, could improve potato tolerance to cold. Potato seedlings, treated or non-treated (0.5 mM) with SNP, were exposed to cold stress (0 and ‒2 °C) for 6 h. The study was conducted in a completely randomized design, incorporating three factors in three replications. Results showed that cold stress reduced physiological and biochemical parameters in all seedlings, but less so in those treated with SNP. SNP treatment boosted physio-biochemical activity and increased levels of soluble sugars and enzymatic and non-enzymatic antioxidants. Seedlings treated with SNP and exposed to cold stress had lower levels of H<sub>2</sub>O<sub>2</sub> and malondialdehyde, suggesting that NO may alleviate the harmful effects of cold. The analysis conducted using PCA demonstrated correlations between variables and treatment groups. Notably, the first two principal components (PC1 and PC2) accounted for 77.6% and 78.1% of total variance, respectively, under both 0 and − 2 °C temperatures. Under temperatures below 0 °C, the results of the factor analysis (FA) revealed that PC1 exhibited the highest distribution of data, containing the most prominent variation in Squared cosine values (SCV) values at 0.79. Among the variables, Electrolyte leakage (EL) had the best representation in PC1, with the corresponding maximum SCV values at 0.78 under − 2 °C. This result highlights the potential use of SNP in manipulating cold tolerance in potato plants.</p>","PeriodicalId":16842,"journal":{"name":"Journal of Plant Growth Regulation","volume":null,"pages":null},"PeriodicalIF":3.9000,"publicationDate":"2024-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Involvement of Nitric Oxide in Biochemical and Physiological Response of Potato Seedling Under Cold Stress\",\"authors\":\"Jafar Nabati, Zahra Nemati, Elaheh Boroumand Rezazadeh\",\"doi\":\"10.1007/s00344-024-11401-z\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p>Cold temperatures harm tropical crops, but understanding how molecular signals help plants cope could aid in climate change adaptation. This study tested if sodium nitroprusside (SNP), a nitric oxide (NO) donor, could improve potato tolerance to cold. Potato seedlings, treated or non-treated (0.5 mM) with SNP, were exposed to cold stress (0 and ‒2 °C) for 6 h. The study was conducted in a completely randomized design, incorporating three factors in three replications. Results showed that cold stress reduced physiological and biochemical parameters in all seedlings, but less so in those treated with SNP. SNP treatment boosted physio-biochemical activity and increased levels of soluble sugars and enzymatic and non-enzymatic antioxidants. Seedlings treated with SNP and exposed to cold stress had lower levels of H<sub>2</sub>O<sub>2</sub> and malondialdehyde, suggesting that NO may alleviate the harmful effects of cold. The analysis conducted using PCA demonstrated correlations between variables and treatment groups. Notably, the first two principal components (PC1 and PC2) accounted for 77.6% and 78.1% of total variance, respectively, under both 0 and − 2 °C temperatures. Under temperatures below 0 °C, the results of the factor analysis (FA) revealed that PC1 exhibited the highest distribution of data, containing the most prominent variation in Squared cosine values (SCV) values at 0.79. Among the variables, Electrolyte leakage (EL) had the best representation in PC1, with the corresponding maximum SCV values at 0.78 under − 2 °C. 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引用次数: 0
摘要
低温会损害热带作物,但了解分子信号如何帮助植物应对低温有助于适应气候变化。本研究测试了一氧化氮(NO)供体硝普钠(SNP)能否提高马铃薯的耐寒性。马铃薯幼苗经 SNP 处理或未经 SNP 处理(0.5 mM),暴露于冷胁迫(0 和 -2 °C)6 小时。结果表明,冷胁迫降低了所有幼苗的生理和生化参数,但用 SNP 处理的幼苗降低幅度较小。SNP 处理提高了生理生化活性,增加了可溶性糖、酶和非酶抗氧化剂的水平。经 SNP 处理并暴露于冷胁迫的幼苗的 H2O2 和丙二醛水平较低,这表明 NO 可减轻冷的有害影响。利用 PCA 进行的分析表明了变量与处理组之间的相关性。值得注意的是,前两个主成分(PC1 和 PC2)在 0 和 - 2 °C 温度下分别占总方差的 77.6% 和 78.1%。在低于 0 °C 的温度条件下,因子分析(FA)结果显示,PC1 的数据分布最高,其平方余弦值(SCV)为 0.79,变化最为显著。在各变量中,电解质渗漏(EL)在 PC1 中的代表性最好,在零下 2 °C 的条件下,相应的最大 SCV 值为 0.78。这一结果凸显了 SNP 在操纵马铃薯植物耐寒性方面的潜在用途。
Involvement of Nitric Oxide in Biochemical and Physiological Response of Potato Seedling Under Cold Stress
Cold temperatures harm tropical crops, but understanding how molecular signals help plants cope could aid in climate change adaptation. This study tested if sodium nitroprusside (SNP), a nitric oxide (NO) donor, could improve potato tolerance to cold. Potato seedlings, treated or non-treated (0.5 mM) with SNP, were exposed to cold stress (0 and ‒2 °C) for 6 h. The study was conducted in a completely randomized design, incorporating three factors in three replications. Results showed that cold stress reduced physiological and biochemical parameters in all seedlings, but less so in those treated with SNP. SNP treatment boosted physio-biochemical activity and increased levels of soluble sugars and enzymatic and non-enzymatic antioxidants. Seedlings treated with SNP and exposed to cold stress had lower levels of H2O2 and malondialdehyde, suggesting that NO may alleviate the harmful effects of cold. The analysis conducted using PCA demonstrated correlations between variables and treatment groups. Notably, the first two principal components (PC1 and PC2) accounted for 77.6% and 78.1% of total variance, respectively, under both 0 and − 2 °C temperatures. Under temperatures below 0 °C, the results of the factor analysis (FA) revealed that PC1 exhibited the highest distribution of data, containing the most prominent variation in Squared cosine values (SCV) values at 0.79. Among the variables, Electrolyte leakage (EL) had the best representation in PC1, with the corresponding maximum SCV values at 0.78 under − 2 °C. This result highlights the potential use of SNP in manipulating cold tolerance in potato plants.
期刊介绍:
The Journal of Plant Growth Regulation is an international publication featuring original articles on all aspects of plant growth and development. We welcome manuscripts reporting question-based research on various aspects of plant growth and development using hormonal, physiological, environmental, genetic, biophysical, developmental and/or molecular approaches.
The journal also publishes timely reviews on highly relevant areas and/or studies in plant growth and development, including interdisciplinary work with an emphasis on plant growth, plant hormones and plant pathology or abiotic stress.
In addition, the journal features occasional thematic issues with special guest editors, as well as brief communications describing novel techniques and meeting reports.
The journal is unlikely to accept manuscripts that are purely descriptive in nature or reports work with simple tissue culture without attempting to investigate the underlying mechanisms of plant growth regulation, those that focus exclusively on microbial communities, or deal with the (elicitation by plant hormones of) synthesis of secondary metabolites.