Izabel Pietczak Migacz, Mei Wang, Janaine Alberto Marangoni Faoro, Sidney Mariano dos Santos, Anelise Samara Nazari Formagio, Candida Aparecida Leite Kassuya, Junaid Ur Rehman, Wilmer Hervet Perera, Vitória Caroline Gonçalves, Douglas Siqueira de Almeida Chaves, Vijayasankar Raman, Paulo Vitor Farago, Ikhlas Ahmed Khan, Jane Manfron
{"title":"叶片生物质的生物勘探和再利用以支持可持续生物制药:六种桉树精油的季节性化学变化和生物活性评估","authors":"Izabel Pietczak Migacz, Mei Wang, Janaine Alberto Marangoni Faoro, Sidney Mariano dos Santos, Anelise Samara Nazari Formagio, Candida Aparecida Leite Kassuya, Junaid Ur Rehman, Wilmer Hervet Perera, Vitória Caroline Gonçalves, Douglas Siqueira de Almeida Chaves, Vijayasankar Raman, Paulo Vitor Farago, Ikhlas Ahmed Khan, Jane Manfron","doi":"10.1007/s41742-024-00628-z","DOIUrl":null,"url":null,"abstract":"<p>This study focuses on six <i>Eucalyptus</i> species, namely <i>E. badjensis</i>, <i>E. benthamii</i>, <i>E. dunnii</i>, <i>E. grandis</i>, <i>E. globulus</i>, and <i>E. saligna</i> aiming at a sustainable use of their green biomass. These species were grown at the same location and stage of development. Leaf essential oils were extracted by hydrodistillation using a Clevenger apparatus during the four seasons. Gas chromatography–mass spectrometry (GC–MS) and high-performance thin-layer chromatography (HPTLC) techniques were used to analyze the chemical composition. <i>E. badjensis</i> consistently exhibited a dominant composition, with 1,8-cineole being the predominant component. Notably, the proportion of 1,8-cineole in <i>E. badjensis</i> was 77.35% in spring (SP), 69.46% in summer (SU), 95.30% in autumn (AU), and 89.30% in winter (WI). <i>E. globulus</i> also exhibited 1,8-cineole as its primary constituent, with proportions fluctuating slightly across seasons at 84.87%, 79.94%, 81%, and 85.88%, respectively. The proportions and constituents of various species differed significantly. HPTLC was successfully used as a swift technique to monitor the chemical composition of essential oils (EOs) in various <i>Eucalyptus</i> species during the seasonality. GC–MS and HPTLC analysis showed that different <i>Eucalyptus</i> species displayed unique chemical compositions, while both the chemical profile and productivity of all analyzed EOs were affected by seasonality. This finding was demonstrated in the principal components cluster. The analysis of six species has revealed that the EO of <i>E. benthamii</i> is the most potent in thwarting the infestation of <i>Cimex lectularius</i>. Additionally, all six EOs demonstrated antioxidant activity in the ABTS model. EOs of <i>E. benthamii</i>, <i>E. dunnii</i>, and <i>E. grandis</i> showed significant anti-inflammatory activities in experimental models.</p>","PeriodicalId":14121,"journal":{"name":"International Journal of Environmental Research","volume":null,"pages":null},"PeriodicalIF":2.6000,"publicationDate":"2024-06-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Bioprospecting and Repurposing of Leaf Biomass to Support Sustainable Biopharmacy: Evaluation of Seasonal Chemical Variations and Biological Activities of Six Eucalyptus Essential Oils\",\"authors\":\"Izabel Pietczak Migacz, Mei Wang, Janaine Alberto Marangoni Faoro, Sidney Mariano dos Santos, Anelise Samara Nazari Formagio, Candida Aparecida Leite Kassuya, Junaid Ur Rehman, Wilmer Hervet Perera, Vitória Caroline Gonçalves, Douglas Siqueira de Almeida Chaves, Vijayasankar Raman, Paulo Vitor Farago, Ikhlas Ahmed Khan, Jane Manfron\",\"doi\":\"10.1007/s41742-024-00628-z\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p>This study focuses on six <i>Eucalyptus</i> species, namely <i>E. badjensis</i>, <i>E. benthamii</i>, <i>E. dunnii</i>, <i>E. grandis</i>, <i>E. globulus</i>, and <i>E. saligna</i> aiming at a sustainable use of their green biomass. These species were grown at the same location and stage of development. Leaf essential oils were extracted by hydrodistillation using a Clevenger apparatus during the four seasons. Gas chromatography–mass spectrometry (GC–MS) and high-performance thin-layer chromatography (HPTLC) techniques were used to analyze the chemical composition. <i>E. badjensis</i> consistently exhibited a dominant composition, with 1,8-cineole being the predominant component. Notably, the proportion of 1,8-cineole in <i>E. badjensis</i> was 77.35% in spring (SP), 69.46% in summer (SU), 95.30% in autumn (AU), and 89.30% in winter (WI). <i>E. globulus</i> also exhibited 1,8-cineole as its primary constituent, with proportions fluctuating slightly across seasons at 84.87%, 79.94%, 81%, and 85.88%, respectively. The proportions and constituents of various species differed significantly. HPTLC was successfully used as a swift technique to monitor the chemical composition of essential oils (EOs) in various <i>Eucalyptus</i> species during the seasonality. GC–MS and HPTLC analysis showed that different <i>Eucalyptus</i> species displayed unique chemical compositions, while both the chemical profile and productivity of all analyzed EOs were affected by seasonality. This finding was demonstrated in the principal components cluster. The analysis of six species has revealed that the EO of <i>E. benthamii</i> is the most potent in thwarting the infestation of <i>Cimex lectularius</i>. Additionally, all six EOs demonstrated antioxidant activity in the ABTS model. EOs of <i>E. benthamii</i>, <i>E. dunnii</i>, and <i>E. grandis</i> showed significant anti-inflammatory activities in experimental models.</p>\",\"PeriodicalId\":14121,\"journal\":{\"name\":\"International Journal of Environmental Research\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":2.6000,\"publicationDate\":\"2024-06-22\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"International Journal of Environmental Research\",\"FirstCategoryId\":\"93\",\"ListUrlMain\":\"https://doi.org/10.1007/s41742-024-00628-z\",\"RegionNum\":4,\"RegionCategory\":\"环境科学与生态学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"ENVIRONMENTAL SCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"International Journal of Environmental Research","FirstCategoryId":"93","ListUrlMain":"https://doi.org/10.1007/s41742-024-00628-z","RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"ENVIRONMENTAL SCIENCES","Score":null,"Total":0}
Bioprospecting and Repurposing of Leaf Biomass to Support Sustainable Biopharmacy: Evaluation of Seasonal Chemical Variations and Biological Activities of Six Eucalyptus Essential Oils
This study focuses on six Eucalyptus species, namely E. badjensis, E. benthamii, E. dunnii, E. grandis, E. globulus, and E. saligna aiming at a sustainable use of their green biomass. These species were grown at the same location and stage of development. Leaf essential oils were extracted by hydrodistillation using a Clevenger apparatus during the four seasons. Gas chromatography–mass spectrometry (GC–MS) and high-performance thin-layer chromatography (HPTLC) techniques were used to analyze the chemical composition. E. badjensis consistently exhibited a dominant composition, with 1,8-cineole being the predominant component. Notably, the proportion of 1,8-cineole in E. badjensis was 77.35% in spring (SP), 69.46% in summer (SU), 95.30% in autumn (AU), and 89.30% in winter (WI). E. globulus also exhibited 1,8-cineole as its primary constituent, with proportions fluctuating slightly across seasons at 84.87%, 79.94%, 81%, and 85.88%, respectively. The proportions and constituents of various species differed significantly. HPTLC was successfully used as a swift technique to monitor the chemical composition of essential oils (EOs) in various Eucalyptus species during the seasonality. GC–MS and HPTLC analysis showed that different Eucalyptus species displayed unique chemical compositions, while both the chemical profile and productivity of all analyzed EOs were affected by seasonality. This finding was demonstrated in the principal components cluster. The analysis of six species has revealed that the EO of E. benthamii is the most potent in thwarting the infestation of Cimex lectularius. Additionally, all six EOs demonstrated antioxidant activity in the ABTS model. EOs of E. benthamii, E. dunnii, and E. grandis showed significant anti-inflammatory activities in experimental models.
期刊介绍:
International Journal of Environmental Research is a multidisciplinary journal concerned with all aspects of environment. In pursuit of these, environmentalist disciplines are invited to contribute their knowledge and experience. International Journal of Environmental Research publishes original research papers, research notes and reviews across the broad field of environment. These include but are not limited to environmental science, environmental engineering, environmental management and planning and environmental design, urban and regional landscape design and natural disaster management. Thus high quality research papers or reviews dealing with any aspect of environment are welcomed. Papers may be theoretical, interpretative or experimental.