转录组分析揭示了耐多药鲍曼不动杆菌中锌介导的毒力和致病性。

3区 生物学 Q1 Biochemistry, Genetics and Molecular Biology
Santhosh M E, Prasanna Kumar Selvam, Mohanraj Gopikrishnan, Karthick Vasudevan, Hatem Zayed, Magesh Ramasamy, Charles Emmanuel Jebaraj Walter, George Priya Doss C
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引用次数: 0

摘要

鲍曼不动杆菌(Acinetobacter baumannii)是一种革兰氏阴性细菌,因其多重耐药性和与 ESKAPE 病原体下的院内感染有关而闻名。这种机会性病原体普遍与医院内感染有关,在医疗保健环境中构成重大威胁。其重要的临床症状,即脑膜炎、尿路感染、血流感染、呼吸机相关肺炎和肺炎,催生了对创新治疗干预措施的迫切需求。锌是一种重要的金属结合蛋白,也是细菌代谢和毒力不可或缺的微量营养素,拟议研究的重点是阐明锌的作用,以加深对鲍曼不动杆菌致病性的了解。研究人员利用 RNA 测序和随后的 DESeq2 分析方法来确定受锌暴露影响的不同基因表达。利用 STRING 数据库进行的功能富集分析表明了锌提高致病性的复杂分子机制。此外,利用 Cytoscape 中的 cytohubba 工具预测了 gltB、ribD、AIL77834.1、sdhB、nuoI、acsA_1、acoC、accA、accD 等枢纽基因。这项研究强调了锌在鲍曼不动杆菌毒力中的关键作用,阐明了导致其致病性的潜在分子途径。这项研究进一步强调了采用创新治疗策略防治鲍曼不动杆菌感染的必要性,尤其是耐多药菌株诱发的感染。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Transcriptomic analysis reveals zinc-mediated virulence and pathogenicity in multidrug-resistant Acinetobacter baumannii.

Acinetobacter baumannii is a gram-negative bacterium well known for its multidrug resistance and connection to nosocomial infections under ESKAPE pathogens. This opportunistic pathogen is ubiquitously associated with nosocomial infections, posing significant threats within healthcare environments. Its critical clinical symptoms, namely, meningitis, urinary tract infections, bloodstream infections, ventilator-associated pneumonia, and pneumonia, catalyze the imperative demand for innovative therapeutic interventions. The proposed research focuses on delineating the role of Zinc, a crucial metallo-binding protein and micronutrient integral to bacterial metabolism and virulence, to enhance understanding of the pathogenicity of A. baumannii. RNA sequencing and subsequent DESeq2 analytical methods were used to identify differential gene expressions influenced by zinc exposure. Exploiting the STRING database for functional enrichment analysis has demonstrated the complex molecular mechanisms underlying the enhancement of pathogenicity prompted by Zinc. Moreover, hub genes like gltB, ribD, AIL77834.1, sdhB, nuoI, acsA_1, acoC, accA, accD were predicted using the cytohubba tool in Cytoscape. This investigation underscores the pivotal role of Zinc in the virulence of A. baumannii elucidates the underlying molecular pathways responsible for its pathogenicity. The research further accentuates the need for innovative therapeutic strategies to combat A. baumannii infections, particularly those induced by multidrug-resistant strains.

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来源期刊
Advances in protein chemistry and structural biology
Advances in protein chemistry and structural biology BIOCHEMISTRY & MOLECULAR BIOLOGY-
CiteScore
7.40
自引率
0.00%
发文量
66
审稿时长
>12 weeks
期刊介绍: Published continuously since 1944, The Advances in Protein Chemistry and Structural Biology series has been the essential resource for protein chemists. Each volume brings forth new information about protocols and analysis of proteins. Each thematically organized volume is guest edited by leading experts in a broad range of protein-related topics.
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