欧米茄-3 可减轻肥胖对 Wistar 大鼠神经传递的干扰,并部分保护肥胖引起的代谢功能障碍。

IF 3.7 3区 医学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY
Gabriel de Farias Fraga, Fernanda da Silva Rodrigues, Jeferson Jantsch, Victor Silva Dias, Vitória Milczarski, Fernanda Wickert, Camila Pereira Medeiros, Sarah Eller, Alethéa Gatto Barschak, Marcia Giovenardi, Renata Padilha Guedes
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引用次数: 0

摘要

奥米加-3(n3)是一种多不饱和脂肪酸,以其抗炎和保护神经的特性而闻名。肥胖与慢性炎症有关,慢性炎症会破坏新陈代谢、肠道生理机能和中枢神经系统功能。本研究旨在确定补充 n3 是否能干扰肥胖对接受食堂饮食(CAF)的 Wistar 大鼠脑内线粒体活性、肠道屏障和神经递质水平的影响。我们检测了四组大鼠的脂肪组织、骨骼肌、血浆、肠道和大脑皮层:CT组(控制饮食)、CTn3组(控制饮食并补充n3)、CAF组和CAFn3组(CAF和n3)。饮食提供 13 周,最后 5 周补充 n3。在 CAF 组中,脂肪组织电子传递链复合物 I、II 和 III 的活性较高,骨骼肌中复合物 III 和 IV 的活性也较高。CAF组血浆中的醋酸盐水平降低,CAF组的脂多糖(LPS)水平升高,而CAFn3组则降低。CAF组肠道中的Claudin-5含量较低,补充n3无影响。在大脑皮层中,CAF组的多巴胺水平降低,而n3则可逆转。多巴胺代谢物 DOPAC 也显示出补充效应,而 HVA 则显示出饮食效应。接受补充剂的 CAF 组血清素水平升高。因此,我们证明了接受 CAF 的大鼠线粒体、血浆、肠道和大脑的紊乱,以及补充 n3 对内毒素血症和神经递质水平的潜在益处。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Omega-3 Attenuates Disrupted Neurotransmission and Partially Protects Metabolic Dysfunction Caused by Obesity in Wistar Rats

Omega-3 Attenuates Disrupted Neurotransmission and Partially Protects Metabolic Dysfunction Caused by Obesity in Wistar Rats

Omega-3 (n3) is a polyunsaturated fatty acid well known for its anti-inflammatory and neuroprotective properties. Obesity is linked to chronic inflammation that disrupts metabolism, the intestine physiology and the central nervous system functioning. This study aims to determine if n3 supplementation can interfere with the effects of obesity on the mitochondrial activity, intestinal barrier, and neurotransmitter levels in the brain of Wistar rats that received cafeteria diet (CAF). We examined adipose tissue, skeletal muscle, plasma, intestine, and the cerebral cortex of four groups: CT (control diet), CTn3 (control diet with n3 supplementation), CAF, and CAFn3 (CAF and n3). Diets were offered for 13 weeks, with n3 supplementation in the final 5 weeks. Adipose tissue Electron Transport Chain complexes I, II, and III showed higher activity in CAF groups, as did complexes III and IV in skeletal muscle. Acetate levels in plasma were reduced in CAF groups, and Lipopolysaccharide (LPS) was higher in the CAF group but reduced in CAFn3 group. Claudin-5 in the intestine was lower in CAF groups, with no n3 supplementation effect. In the cerebral cortex, dopamine levels were decreased with CAF, which was reversed by n3. DOPAC, a dopamine metabolite, also showed a supplementation effect, and HVA, a diet effect. Serotonin levels increased in the CAF group that received supplementation. Therefore, we demonstrate disturbances in mitochondria, plasma, intestine and brain of rats submitted to CAF and the potential benefit of n3 supplementation in endotoxemia and neurotransmitter levels.

Graphical Abstract

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来源期刊
Neurochemical Research
Neurochemical Research 医学-神经科学
CiteScore
7.70
自引率
2.30%
发文量
320
审稿时长
6 months
期刊介绍: Neurochemical Research is devoted to the rapid publication of studies that use neurochemical methodology in research on nervous system structure and function. The journal publishes original reports of experimental and clinical research results, perceptive reviews of significant problem areas in the neurosciences, brief comments of a methodological or interpretive nature, and research summaries conducted by leading scientists whose works are not readily available in English.
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