Ibrahim B Halidu, Amos X Gafa, Samuel D K Blanney, Benjamin T Barimah, David Akan-Enge, Joseph Boachie, Kate A Kontor, Patrick Adu
{"title":"加纳南部和北部选定实验室的血液学结果缺乏共识。","authors":"Ibrahim B Halidu, Amos X Gafa, Samuel D K Blanney, Benjamin T Barimah, David Akan-Enge, Joseph Boachie, Kate A Kontor, Patrick Adu","doi":"10.4314/gmj.v57i3.8","DOIUrl":null,"url":null,"abstract":"<p><strong>Objective: </strong>To assess the inter-laboratory comparability and intra-assay reproducibility of full blood count (FBC) results.</p><p><strong>Design: </strong>Exploratory cross-sectional study.</p><p><strong>Setting: </strong>Three and two selected medical laboratories in the northern and southern zones, respectively.</p><p><strong>Participants: </strong>Forty-nine individuals per zone; 16 type 2 diabetes mellitus, 16 with HbAS haemoglobin type and 17 normal samples.</p><p><strong>Intervention: </strong>Each sample was run eleven times through the analysers in the participating laboratories to evaluate intra-laboratory reproducibility and comparability of FBC results.</p><p><strong>Main outcome measure: </strong>Intra-laboratory reproducibility was evaluated using %coefficient variation (%CV). Interlaboratory comparisons were assessed through t-test or One-Way ANOVA for two-sample and three-sample tests. All statistical testing was undertaken using the two-tailed assumption.</p><p><strong>Result: </strong>Statistically significantly different haemoglobin levels were estimated in both northern and southern zones (mean difference 0.00 g/dL to 3.75 g/dL vs 0.18 g/dL to 1.92 g/dL respectively). Also, total WBC counts significantly differed across laboratories in both northern and southern zones (mean difference 0.15 ×10<sup>9</sup>/L - 3.86 ×10<sup>9</sup>/L vs 0.02 ×10<sup>9</sup>/L to 1.39 ×10<sup>9</sup>/L respectively). Furthermore, platelet counts significantly differed across the participating laboratories in the northern and southern zones (mean difference 0.40 ×10<sup>9</sup>/L to 299.76 ×10<sup>9</sup>/L vs 5.7 ×10<sup>9</sup>/L to 76.9 ×10<sup>9</sup>/L respectively). Moreover, there was evidence of non-reproducibility of results within the respective laboratories in each zone as the respective %CV were outside the acceptable limits.</p><p><strong>Conclusion: </strong>The intra-laboratory non-reproducibility and inter-laboratory non-comparability of FBC results highlight the need to establish a national quality assessment scheme to harmonise laboratory practices nationwide.</p><p><strong>Funding: </strong>This study was funded by the University of Cape Coast Individual-Led Research Support Grant (RSG-INDI-CoHAS-2019-107).</p>","PeriodicalId":94319,"journal":{"name":"Ghana medical journal","volume":"57 3","pages":"210-217"},"PeriodicalIF":0.0000,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11216730/pdf/","citationCount":"0","resultStr":"{\"title\":\"Lack of consensus in inter-laboratory haematology results in selected laboratories in the southern and northern zones of Ghana.\",\"authors\":\"Ibrahim B Halidu, Amos X Gafa, Samuel D K Blanney, Benjamin T Barimah, David Akan-Enge, Joseph Boachie, Kate A Kontor, Patrick Adu\",\"doi\":\"10.4314/gmj.v57i3.8\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Objective: </strong>To assess the inter-laboratory comparability and intra-assay reproducibility of full blood count (FBC) results.</p><p><strong>Design: </strong>Exploratory cross-sectional study.</p><p><strong>Setting: </strong>Three and two selected medical laboratories in the northern and southern zones, respectively.</p><p><strong>Participants: </strong>Forty-nine individuals per zone; 16 type 2 diabetes mellitus, 16 with HbAS haemoglobin type and 17 normal samples.</p><p><strong>Intervention: </strong>Each sample was run eleven times through the analysers in the participating laboratories to evaluate intra-laboratory reproducibility and comparability of FBC results.</p><p><strong>Main outcome measure: </strong>Intra-laboratory reproducibility was evaluated using %coefficient variation (%CV). Interlaboratory comparisons were assessed through t-test or One-Way ANOVA for two-sample and three-sample tests. All statistical testing was undertaken using the two-tailed assumption.</p><p><strong>Result: </strong>Statistically significantly different haemoglobin levels were estimated in both northern and southern zones (mean difference 0.00 g/dL to 3.75 g/dL vs 0.18 g/dL to 1.92 g/dL respectively). Also, total WBC counts significantly differed across laboratories in both northern and southern zones (mean difference 0.15 ×10<sup>9</sup>/L - 3.86 ×10<sup>9</sup>/L vs 0.02 ×10<sup>9</sup>/L to 1.39 ×10<sup>9</sup>/L respectively). Furthermore, platelet counts significantly differed across the participating laboratories in the northern and southern zones (mean difference 0.40 ×10<sup>9</sup>/L to 299.76 ×10<sup>9</sup>/L vs 5.7 ×10<sup>9</sup>/L to 76.9 ×10<sup>9</sup>/L respectively). Moreover, there was evidence of non-reproducibility of results within the respective laboratories in each zone as the respective %CV were outside the acceptable limits.</p><p><strong>Conclusion: </strong>The intra-laboratory non-reproducibility and inter-laboratory non-comparability of FBC results highlight the need to establish a national quality assessment scheme to harmonise laboratory practices nationwide.</p><p><strong>Funding: </strong>This study was funded by the University of Cape Coast Individual-Led Research Support Grant (RSG-INDI-CoHAS-2019-107).</p>\",\"PeriodicalId\":94319,\"journal\":{\"name\":\"Ghana medical journal\",\"volume\":\"57 3\",\"pages\":\"210-217\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2023-09-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11216730/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Ghana medical journal\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.4314/gmj.v57i3.8\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Ghana medical journal","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.4314/gmj.v57i3.8","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Lack of consensus in inter-laboratory haematology results in selected laboratories in the southern and northern zones of Ghana.
Objective: To assess the inter-laboratory comparability and intra-assay reproducibility of full blood count (FBC) results.
Design: Exploratory cross-sectional study.
Setting: Three and two selected medical laboratories in the northern and southern zones, respectively.
Participants: Forty-nine individuals per zone; 16 type 2 diabetes mellitus, 16 with HbAS haemoglobin type and 17 normal samples.
Intervention: Each sample was run eleven times through the analysers in the participating laboratories to evaluate intra-laboratory reproducibility and comparability of FBC results.
Main outcome measure: Intra-laboratory reproducibility was evaluated using %coefficient variation (%CV). Interlaboratory comparisons were assessed through t-test or One-Way ANOVA for two-sample and three-sample tests. All statistical testing was undertaken using the two-tailed assumption.
Result: Statistically significantly different haemoglobin levels were estimated in both northern and southern zones (mean difference 0.00 g/dL to 3.75 g/dL vs 0.18 g/dL to 1.92 g/dL respectively). Also, total WBC counts significantly differed across laboratories in both northern and southern zones (mean difference 0.15 ×109/L - 3.86 ×109/L vs 0.02 ×109/L to 1.39 ×109/L respectively). Furthermore, platelet counts significantly differed across the participating laboratories in the northern and southern zones (mean difference 0.40 ×109/L to 299.76 ×109/L vs 5.7 ×109/L to 76.9 ×109/L respectively). Moreover, there was evidence of non-reproducibility of results within the respective laboratories in each zone as the respective %CV were outside the acceptable limits.
Conclusion: The intra-laboratory non-reproducibility and inter-laboratory non-comparability of FBC results highlight the need to establish a national quality assessment scheme to harmonise laboratory practices nationwide.
Funding: This study was funded by the University of Cape Coast Individual-Led Research Support Grant (RSG-INDI-CoHAS-2019-107).
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