手性胺合成的酶法路线:蛋白质工程与工艺优化。

IF 5.3 Q1 MEDICINE, RESEARCH & EXPERIMENTAL
Biologics : Targets & Therapy Pub Date : 2024-06-25 eCollection Date: 2024-01-01 DOI:10.2147/BTT.S446712
Sayali Shantaram Vikhrankar, Seema Satbhai, Priyanka Kulkarni, Ranjit Ranbhor, Vibin Ramakrishnan, Prashant Kodgire
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引用次数: 0

摘要

手性胺是制药、农用化学品和特种化学品中的重要基团。传统的化学方法制备手性胺往往缺乏立体选择性,而且需要苛刻的条件,而使用工程酶的生物催化方法可以在可持续的条件下提供高效率和高选择性。本综述讨论了为提高催化性能而对转氨酶、氧化酶和其他酶进行蛋白质工程改造的最新进展。定向进化、固定化和计算重新设计等策略扩大了底物范围并提高了效率。此外,以技术经济评估为指导的工艺优化对于建立可行的生物制造路线至关重要。将最先进的酶工程技术与多方面的工艺开发相结合,将实现对各种手性胺目标的可扩展、经济的酶法合成。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Enzymatic Routes for Chiral Amine Synthesis: Protein Engineering and Process Optimization.

Chiral amines are essential motifs in pharmaceuticals, agrochemicals, and specialty chemicals. While traditional chemical routes to chiral amines often lack stereoselectivity and require harsh conditions, biocatalytic methods using engineered enzymes can offer high efficiency and selectivity under sustainable conditions. This review discusses recent advances in protein engineering of transaminases, oxidases, and other enzymes to improve catalytic performance. Strategies such as directed evolution, immobilization, and computational redesign have expanded substrate scope and enhanced efficiency. Furthermore, process optimization guided by techno-economic assessments has been crucial for establishing viable biomanufacturing routes. Combining state-of-the-art enzyme engineering with multifaceted process development will enable scalable, economical enzymatic synthesis of diverse chiral amine targets.

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来源期刊
Biologics : Targets & Therapy
Biologics : Targets & Therapy MEDICINE, RESEARCH & EXPERIMENTAL-
CiteScore
8.30
自引率
0.00%
发文量
22
审稿时长
16 weeks
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