大型萜烯合成酶的鉴定和功能/结构分析。

4区生物学 Q3 Biochemistry, Genetics and Molecular Biology

Methods in enzymology Pub Date : 2024-01-01 Epub Date: 2024-04-09 DOI:10.1016/bs.mie.2024.03.017

Daijiro Ueda, Tohru Abe, Masahiro Fujihashi, Tsutomu Sato

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引用次数: 0

摘要

大萜烯合成酶（large-TSs）是一个新的 TS 家族。第一个被发现的大型萜合成酶来自枯草芽孢杆菌（BsuTS），它参与 C35 倍半萜的生物合成。大型 TS 是唯一能进行倍半萜生物合成的酶，与典型的 I 类和 II 类 TS 没有任何序列同源性。因此，对大型 TS 的研究有望拓展萜烯领域的化学空间。在本章中，我们将介绍用于鉴定大 TSs 的实验方法及其功能和结构分析。此外，还介绍了与大-TS 底物生物合成相关的几种酶。

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Identification and functional/structural analyses of large terpene synthases.

Large terpene synthases (large-TSs) are a new family of TSs. The first large-TS discovered was from Bacillus subtilis (BsuTS), which is involved in the biosynthesis of a C₃₅ sesquarterpene. Large-TSs are the only enzymes that enable the biosynthesis of sesquarterpenes and do not share any sequence homology with canonical Class I and II TSs. Thus, the investigation of large-TSs is promising for expanding the chemical space in the terpene field. In this chapter, we describe the experimental methods used for identifying large-TSs, as well as their functional and structural analyses. Additionally, several enzymes related to the biosynthesis of large-TS substrates have been described.

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来源期刊

Methods in enzymology 生物-生化研究方法

CiteScore

2.90

自引率

0.00%

发文量

308

审稿时长

3-6 weeks

期刊介绍： The critically acclaimed laboratory standard for almost 50 years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Each volume is eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with over 500 volumes the series contains much material still relevant today and is truly an essential publication for researchers in all fields of life sciences, including microbiology, biochemistry, cancer research and genetics-just to name a few. Five of the 2013 Nobel Laureates have edited or contributed to volumes of MIE.