参与真菌经萜生物合成的跨膜萜环酶的功能分析。

4区 生物学 Q3 Biochemistry, Genetics and Molecular Biology
Methods in enzymology Pub Date : 2024-01-01 Epub Date: 2024-02-24 DOI:10.1016/bs.mie.2024.02.007
Jia Tang, Yudai Matsuda
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引用次数: 0

摘要

Pyr4 家族萜烯环化酶是非典型的跨膜 II 类萜烯环化酶,可催化微生物萜类化合物(如美拉萜类化合物)生物合成过程中的各种环化反应。然而,虽然这些环化酶广泛分布于微生物中,但它们的三维结构尚未确定,这可能是由于这些酶的跨膜位置所致。在本章中,我们介绍了根据使用 AlphaFold2 生成的模型结构对跨膜萜环酶进行功能分析的程序。我们以 Pyr4 家族的萜烯环化酶 AdrI 为例,该酶是生物合成 andrastin A 及其同源物所必需的。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Functional analysis of transmembrane terpene cyclases involved in fungal meroterpenoid biosynthesis.

Pyr4-family terpene cyclases are noncanonical transmembrane class II terpene cyclases that catalyze a variety of cyclization reactions in the biosynthesis of microbial terpenoids, such as meroterpenoids. However, although these cyclases are widely distributed in microorganisms, their three-dimensional structures have not been determined, possibly due to the transmembrane locations of these enzymes. In this chapter, we describe procedures for the functional analysis of transmembrane terpene cyclases based on their model structures generated using AlphaFold2. We used AdrI, the Pyr4-family terpene cyclase required for the biosynthesis of andrastin A and its homologs, as an example.

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来源期刊
Methods in enzymology
Methods in enzymology 生物-生化研究方法
CiteScore
2.90
自引率
0.00%
发文量
308
审稿时长
3-6 weeks
期刊介绍: The critically acclaimed laboratory standard for almost 50 years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Each volume is eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with over 500 volumes the series contains much material still relevant today and is truly an essential publication for researchers in all fields of life sciences, including microbiology, biochemistry, cancer research and genetics-just to name a few. Five of the 2013 Nobel Laureates have edited or contributed to volumes of MIE.
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